SNAP23 antibody

Principal name

SNAP23 antibody

Alternative names for SNAP23 antibody

SNAP-23, Synaptosomal-associated protein 23, Vesicle-membrane fusion protein SNAP-23

SwissProt ID

O00161 (Human), O09044 (Mouse), O70377 (Rat)

Gene ID

8773 (SNAP23), 20619 (Snap23), 64630

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of SNAP23 antibody

SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

General readings

1. Guo, Z., Turner, C., Castle, D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell
surface projections regulates compound exocytosis in mast cells. Cell 94: 537-548, 1998.
2. Lazo, P. A., Nadal, M., Ferrer, M., Area, E., Hernandez-Torres, J., Nabokina, S. M., Mollinedo, F.,
Estivill, X. Genomic organization, chromosomal localization, alternative splicing, and isoforms of the
human synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion
processes. Hum. Genet. 108: 211-215, 2001.
3. Mollinedo, F., Lazo, P. A. Identification of two isoforms of the vesicle-membrane fusion protein
SNAP-23 in human neutrophils and HL-60 cells. Biochem. Biophys. Res. Commun. 231: 808-812,
1997.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SNAP23 (1-132) antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

SNAP23 antibody

AP21296PU-N SNAP23 antibody staining of Human Placenta lysate at 0.1µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SNAP23 (C-term) antibody

(0.2ug/ml) staining of PBMC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rb, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SNAP23 antibody

WB Suggested Anti-SNAP23 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SNAP23 antibody

Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-6: Human placenta tissue, hela cells, hepg2 cells, human fetal liver tissue, A549 cells, human normal kidney tissue, Primary antibody: (SNAP23 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SNAP23 antibody

Tonsil stained in IHC-P with SNAP23 antibody Rabbit Purified Hu E, ICC/IF, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SNAP23 antibody

SNAP23 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SNAP23 antibody

Western Blot analysis of SNAP23 expression in transfected 293T cell line (H00008773-T01) by SNAP23 MaxPab polyclonal antibody.

Lane 1: SNAP23 transfected lysate(23.21 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SNAP23 antibody

Western Blot analysis of SNAP23 expression in transfected 293T cell line (H00008773-T02) by SNAP23 MaxPab polyclonal antibody.

Lane 1: SNAP23 transfected lysate(23.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SNAP23 antibody

Immunoperoxidase of monoclonal antibody to SNAP23 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml] Mouse IgG1 2F5-3D4 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SNAP23 antibody

Human Jurkat; WB Suggested Anti-SNAP23 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; SNAP23 antibody - N-terminal region (ARP48615_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SNAP23 antibody

SNAP23 Rabbit IgG Aff - Purified Hu, Ms, Rt C, P, WB
0.1 mg / €290.00
  Boster Immunoleader

CRISPR

Catalog No. Species Donor Vector  

Snap23 - mouse gene knockout kit via CRISPR

Snap23 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Snap23 - mouse gene knockout kit via CRISPR

Snap23 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Snap23 - mouse gene knockout kit via CRISPR

Snap23 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SNAP23 (transcript variant 1)

SNAP23 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

SNAP23 (1-211)

SNAP23 Human Purified > 90 % by SDS PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

SNAP23 (1-211)

SNAP23 Human Purified > 90 % by SDS PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

SNAP23

SNAP23 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SNAP23

SNAP23 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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