SNAP25 antibody

Principal name

SNAP25 antibody

Alternative names for SNAP25 antibody

SNAP-25, SUP, SNAP, Super Protein, RIC4, ric-4, Synaptosomal-associated protein 25, Synaptosomal-associated 25 kDa protein

SwissProt ID

O62414 (Caeel), P36975 (Drome), P55820 (Rabit), P60878 (Chick), P60879 (Mouse), P60880 (Human), P60881 (Rat), Q17QQ3 (Bovin), Q8IXK3 (Human), Q96FM2 (Mouse)

Gene ID

6616 (SNAP25), 25012 (Snap25), 179427, 20614

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey), Por (Porcine), Can (Canine), GP (Guinea Pig), Ze (Zebrafish), Hst (Hamster), Bac (Bacteria), Ce (C. elegans), Prim (Primate), Fe (Feline)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of SNAP25 antibody

SP-25 (or syptosomal-associated protein of 25kD) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SRE) protein superfamily. The family includes 36 members in humans and is characterized by the SRE motif, an evolutiorily conserved stretch of 60–70 amino acids that are arranged in heptad repeats. SRE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion. SREs can be roughly divided into vesicular (v-SREs) and target (t-SREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cogte v-/t-SREs between two opposing membranes generates trans-SRE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion. SP-25, unlike most SREs, lacks a membrane-spanning region, but is attached to the cytoplasmic side of the plasma membrane by posttranslatiol modifications such as palmitoylation or farnesylation. SP-25 has been extensively studied for its role on neurol and neuroendocrine cell exocytosis where it functions as one of the plasma membrane protein t-SRE, which together with the vesicular v-SRE protein VAMP and another t-SRE such as Syntaxin 1, forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers. SP-25 is the target of several botulinum neurotoxin (BoNT) types: type A, C and E. The neurotoxins cause specific proteolytic degradation of the SP-25 protein, which in turn causes SRE complex disruption and inhibition of neurotransmitter release.

General readings

General / background references:Honer WG et al. Hippocampal synaptic pathology in patients with temporal lobe epilepsy. Acta Neuropathol (Berl) 87:202-10 (1994). PubMed PMID: 8171971
Honer WG et al. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res 609:9-20 (1993). PubMed PMID: 7685234
Honer WG et al. Regional synaptic pathology in Alzheimer's disease. Neurobiol Aging 13:375-82 (1992). PubMed PMID: 1625766
Honer WG et al. Characterization of a synaptic antigen of interest in neuropsychiatric illness. Biol Psychiatry 31:147-58 (1992). PubMed PMID: 1737076

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SNAP25 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502838) Mouse IgG2a OTI 1D5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502884) Mouse IgG2a OTI1D3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502963) Mouse IgG2a OTI4G5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502965) Mouse IgG2b OTI 1E6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502967) Mouse IgG1 OTI1G2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502968) Mouse IgG2a OTI4B2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502970) Mouse IgG2a OTI4B5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 (C-term) antibody

TA303020 (0.03µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SNAP25 (C-term) antibody

Western blot of rat cortex lysate showing specific labeling of the ~25k SNAP25 protein. Rabbit IgG Aff - Purified Bov, Can, Chk, GP, Hu, Ms, non-Human Primates, Rt, Ze WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SNAP25 antibody

Predicted band size: 23 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SNAP25 antibody

Predicted band size: 23 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SNAP25 antibody

Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using SNAP25 antibody (#TA326256).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SNAP25 antibody

Western blot analysis of SNAP25 in a human cell line mix using a 1:1000 dilution of the antibody Mouse IgG1 SP-12 Purified Fe, Hu, Ms, Por, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SNAP25 antibody

Western blot analysis of extracts of various cell lines, using SNAP25 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SNAP25 antibody

Western blot analysis of rat brain membranes: 1. Anti-SNAP-25 antibody, (1:1000). 2. Anti-SNAP-25 antibody, preincubated with the control fusion protein antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SNAP25 antibody

Western blot analysis on RAW264.7 cell lysate using SNAP25 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SNAP25 antibody

Anti-SNAP25 mouse monoclonal antibody (TA502964) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25(RC202068). Mouse IgG2a OTI4C6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

Anti-SNAP25 mouse monoclonal antibody (TA502966) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25(RC202068). Mouse IgG2a OTI4F3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SNAP25 antibody

SNAP25 Mouse IgG1 S-7B8 Purified Bov, Chk, Hu, Mky, Ms, Rt P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

SNAP25 antibody

SNAP25 Mouse IgG1 B-29-F9 Purified Bov, Chk, Hu, Mky, Ms, Rt P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

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