SNAP91 antibody

Principal name

SNAP91 antibody

Alternative names for SNAP91 antibody

SNAP-91, Protein AP180, KIAA0656, Clathrin coat assembly protein AP180, Clathrin coat-associated protein AP180, 91 kDa synaptosomal-associated protein, Phosphoprotein F1-20

SwissProt ID

O60641 (Human), Q05140 (Rat), Q61548 (Mouse)

Gene ID

9892 (SNAP91)

Available reactivities

Hu (Human), Bov (Bovine), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C)

Background of SNAP91 antibody

Clathrin-coated membranes of eukaryotic cells participate in various intracellular transport processes, such as receptor-mediated endocytosis, the retrieval of membrane after exocytosis, the routing of lysosomal enzymes from the trans-Golgi network to a prelysosomal compartment, and the maturation of prohormone-filled exocytotic storage vesicles. Vesicle transport is preceded by polymerization of clathrin protomers on the membrane leading to extended regular coats. These generate coated vesicles by a process of budding and severing. The ability to polymerize into regular coat structures is an inherent property of clathrin triskelia. However, it is strictly dependent on protein cofactors which are released from the coated vesicle membrane with clathrin in 0.5 M Tris. Four clathrin-binding proteins have been identified that support clathrin assembly in vitro. These are the AP1 and AP2 adaptor protein complexes, also known as HAI (HA1) adaptor and HAII (HA2) adaptor or Assembly Protein 1 (AP1) and Assembly Protein 2 (AP2), respectively, AP180 (AP3) and auxilin. Based on results obtained by molecular cloning and sequencing, the molecular weight of AP180 is 92 kD. Its sequence is unrelated to that of any other known protein. Its expression is probably restricted to cells of 7 neuronal origin and the two neuronal proteins AP-3 and NP185 were shown to be identical with AP180. AP180 migrates in standard Laemmli-type SDS PAGE as a 170-190 kD polypeptide and is therefore difficult to resolve from the clathrin heavy chain. In polyacrylamide gels with a low proportion of crosslinker, AP180 separates well from the clathrin heavy chain, which behaves in this electrophoretic system like an approx. 150 kD polypeptide. AP180 binds to clathrin triskelia with a stoichiometry of one per clathrin triskelion (trimer of heavy chains) and thereby induces assembly of clathrin into polyhedral cages. Monoclonal antibodies which react specifically with coated vesicle proteins, are useful for studies on the intracellular distribution and structural relationship of clathrin-binding proteins.

General readings

1. Krauss, M., et al., PNAS 103, 11934-11939 (2006)
2. Kalthoff, C., et al., J. Biol. Chem. 277, 8209-8216 (2002)
3. Paolo, G. D., et al., Neuron 33, 789-804 (2002)
4. Hao, W., et al., J. Biol. Chem. 274, 22785-22794 (1999)
Clone LP2D11 is used in these references.

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SNAP91 antibody

SNAP91 polyclonal antibody (A01), Lot # 060707JCS1. Western Blot analysis of SNAP91 expression in Daoy. (Isoform : 63.128 kDa) Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SNAP91 antibody

Western Blot analysis of SNAP91 expression in transfected 293T cell line (H00009892-T01) by SNAP91 MaxPab polyclonal antibody.

Lane 1: SNAP91 transfected lysate(99.77 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SNAP91 antibody

Western Blot analysis of SNAP91 expression in transfected 293T cell line (H00009892-T01) by SNAP91 MaxPab polyclonal antibody.

Lane 1: SNAP91 transfected lysate(99.77 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

SNAP91 antibody

SNAP91 Rabbit Purified Hu, Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SNAP91 antibody

SNAP91 Mouse IgG LP2D11 Purified Bov, Hu, Ms, Rt C, ICC/IF, IP, WB
50 µg / €320.00
  Novus Biologicals Inc.

SNAP91 antibody

Immunohistochemistry-Paraffin: AP180 Antibody [NBP1-90146] Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells and strong staining in neuropil. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

SNAP91 antibody

Immunohistochemistry-Paraffin: AP180 Antibody [NBP1-90147] Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity neuronal cells and strong staining in neuropil. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SNAP91 (transcript variant 1)

SNAP91 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SNAP91

SNAP91 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SNAP91

SNAP91 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SNAP91

SNAP91 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SNAP91

SNAP91 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SNAP91 293T Cell Transient Overexpression Lysate(Denatured)

SNAP91 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

SNAP91 overexpression lysate

SNAP91 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

14 Item(s)

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