SOCS1 antibody

Principal name

SOCS1 antibody

Alternative names for SOCS1 antibody

Suppressor of cytokine signaling 1, SOCS-1, SSI1, SSI-1, TIP3, TIP-3, JAB, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3

SwissProt ID

O15524 (Human), O35716 (Mouse), Q9QX78 (Rat)

Gene ID

8651 (SOCS1), 252971 (Socs1), 12703

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Chk (Chicken), Rt (Rat), Rb (Rabbit), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SOCS1 antibody

Suppressor of cytokine signaling 1 (SOCS1) is a member of the SOCS family of proteins, consisting of eight members (SOCS1 to SOCS7 and CIS) all sharing a central SH2 domain and a c-terminal SOCS box (1). This family is involved in inducible feedback inhibition of janus kinases (JAKs) and signal transducers and activators of transcription (STATs) signaling pathway, which plays multiple roles in cell growth, differentiation, and apoptosis (2). SOCS1 is located on chromosome 16 (3). It plays a key role in the negative regulation of IFN-gamma signaling and in T-cell differentiation. SOCS1 inhibits macrophage differentiation of M1 cells in response to IL-6, protects protein tyrosine phosphatases by thioredoxin upregulation and attenuates JAKs to suppress ROS-mediated apoptosis (2, 4). It translocates into the nucleus due to the presence of a functional nuclear localization signal (NLS). It also regulates IFN-gamma mediated sensory neuronal survival.
SOCS1 is implicated in various cancers and diseases. Mutations of SOCS1 are found in classic Hodgkin lymphoma and primary mediastinal B-cell lymphoma (5). It is induced in response to TNF-alpha or H2O2 and by a subset of cytokines including those belonging to interferon, interleukin and colony stimulating factor colonies. It attenuates their signaling by its ability to bind and inhibit all four of the JAKs. It is found to colocalize and biochemically copurify with the microtubule organizing complex (MTOC) and its associated 20S proteasome (6). It is expressed in all tissues with high expression in spleen, small intestine and peripheral blood lymphocytes. It is approximately a 25kDa protein (211 amino acids).

General readings

1. Larsen L, Ropke C. Suppressors of Cytokine Signaling: SOCS. APMIS. December 2002; 110(12):833-844.
2. Oh J, Hur MW, Lee CE. SOCS1 protects protein tyrosine phosphatases by thioredoxin upregulation and attenuates JAK to suppress ROS mediated apoptosis. Oncogene. 3 September 2009; 28(35):3145-3156.
3. Yandava CN, Pillari A, Drazen JM. Radiation hybrid and cytogenetic mapping of SOCS1 and SOCS2 to chromosomes 16p13 and 12q, respectively. Genomics. 1 October 1999; 61(1):108-111.
4. Starr R, Wilson TA, Viney EM, Murray LJ, Rayner JR, Jenkins BJ, Gonda TJ, Alexander WS, Metcalf D, Nicola NA, Hilton DJ. A family of cytokine-inducible inhibitors of signaling. Nature. 26 June 1997; 6636:917-921.
5. Weniger MA, Melzner I, Menz CK, Wegener S, Bucur AJ, Dorsch K, Mattfeldt T, Barth TF, Muller P. Mutations of the tumor suppressor gene SOCS-1 in classical Hodgkin lymphoma are frequent and associated with delayed degradation of JAK2 protein. Oncogene. 2006; 25(18):2679-2684.
6. Vuong BQ, Arenzana TL, Showalter BM, Losman J, Chen XP, Mostecki J, Banks AS, Limnander A, Fernandez N, Rothman PB. SOCS-1 localizes to the microtubule organizing complexassociated
20S proteasome. Mol Cell Biol. 2004; 24(20):9092-9101.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SOCS1 (C-term) antibody

  Goat Polyclonal Antibody against SOCS1  
TA302691 staining (2µg/ml) of mouse spleen lysate (RIPA buffer,  35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SOCS1 (C-term) antibody

  Rabbit Polyclonal SOCS1 Antibody  
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SOCS1 (N-term) antibody

  Rabbit Polyclonal SOCS1 Antibody  
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SOCS1 antibody

  Anti-SOCS1 rabbit polyclonal antibody  
Predicted band size: 24 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SOCS1 antibody

  Anti-SOCS1 rabbit polyclonal antibody  
Predicted band size: 24kDa. Positive control: HT-29 and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HT-29 cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SOCS1 antibody

  Rabbit Polyclonal SOCS-1 Antibody  
Western Blot: SOCS1 Antibody [TA336433] - Western blot analysis of SOCS1 using TA336433 at 1:1000 dilution against 15 ug/lane of Ramos (A) and Raw (B) cell lysate. Rabbit Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

SOCS1 (2-78) antibody

Western blot analysis of GST-SOCS-1 (2-78 a.a.) using AM26561AF-N. Mouse IgG1 4H1 Azide Free Ms IP, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

SOCS1 (2-78) antibody

Immunoprecipitation of SOCS-1 from mouse splenocytes with mouse IgG1 (1), AM26561AF-N (2), and from mouse hepatocytes with mouse IgG1 (3), AM26561AF-N (4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26561BT-N. Mouse IgG1 4H1 Biotin Ms WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SOCS1 antibody

Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SOCS1 antibody

Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SOCS1 Control Peptide

SOCS1
0.1 mg / €250.00
  Acris Antibodies GmbH

SOCS1 antibody

SOCS1 Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SOCS1 (N-term) antibody

Western blot analysis of SOCS1 Antibody (N-term) (AP18204PU-N) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SOCS1 antibody

Western blot analysis of SOCS-1 in extracts from Jurkat cells using SOCS1 / JAB antibody (Cat.-No. AP20630PU-N). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SOCS1 (C-term) antibody

Immunohistochemical analysis of paraffin-embedded rat tissue sections (lung), staining SOCS1 in nucleus, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SOCS1 - human gene knockout kit via CRISPR

SOCS1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SOCS1 - human gene knockout kit via CRISPR

SOCS1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SOCS1 - human gene knockout kit via CRISPR

SOCS1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SOCS1 - human gene knockout kit via CRISPR

SOCS1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SOCS1

  Recombinant protein of human suppressor of cytokine signaling 1 (SOCS1)  
SOCS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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