SOCS3 / CIS3 antibody

Principal name

SOCS3 / CIS3 antibody

Alternative names for SOCS3 / CIS3 antibody

SOCS-3, CIS-3, SSI3, SSI-3, Suppressor of cytokine signaling 3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3

SwissProt ID

O14543 (Human), O35718 (Mouse), O88583 (Rat), Q68AM8 (Canfa), Q90X67 (Chick), Q9BEG9 (Bovin)

Gene ID

9021 (SOCS3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Mky (Monkey), Chk (Chicken), Sh (Sheep)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of SOCS3 / CIS3 antibody

Suppressors of cytokine signaling (SOCS) proteins are intracellular regulators of receptor signal transduction, mainly Janus kinase/signal transducers and activators of transcription (JAK/STAT). The suppressor for cytokine signaling 3 is a key regulator of cytokine signaling in inflammatory diseases and in breast cancer progression. Over expression of SOCS3 in response to prolactin, suppresses STAT3 expression and abrogate STAT5 phosphorylation which resulted in decrease cell proliferation of T47D breast cancer cells. SOCS3 acts as an anti-proliferative agent in breast cancer cells whose mechanism of action and cross talk with other cellular pathways are complex (1). The IL6 signaling on both hepatic and hematopoietic cells is mediated through gp130 receptor and SOCS3 is a critical inhibitor of this pathway (2). SOCS3 knockout mice revealed significant enhancement in DNA replication and liver weight restoration after partial hepatectomy (PH) compared to littermate controls. The absence of SOCS3, STAT3 phosphorylation is prolonged activation of ERK1/2 is enhances after PH. SOCS protein inhibit cytokine signaling by targeting different component of signaling complex. These include either direct binding of SOCS to activated , tyrosine phosphorylated cytokine receptors ot JAK via their Src homology 2 doamin or via targeting receptor complexes for proteosomal degradation via SOC box (3). SOCS3 has been shown to regulate the activity of proline rich tyrosine kinase 2 (PYK2) in A549 cells as a result of epigenetic regulatory mechanism involving methylation of exon 2, and 5. Recent literature suggest that infections (hepatitis C and Leishmania donovani) caused an induction is SOCS3 that interfere with host immune responses.
There are eight SOCS family members known, SOCS1, SOCS2 and SOCS3, SOCS2 can enhance IL2 and IL3 induced STAT phosphorylation followed bt potentiate proliferation in response to cytokine stimulation. Expression of SOCS2 also resulted in marked proteosome-dependent reduction in SOCS3 and SOCS1 proteins. SOCS2 also bind to SOCS3 and elongin B/C to form an E3 ligase complex resulting in degradation of SOCS3 (4). SOCS3 is localized on chromosome 17q25 and was found to be expressed at higher concentration in skin of atopic dermatitis patients than in healthy individuals (5). SOCS3 is a 225 amino acid containing protein with apparent MW 28kDa. SOCS3 protein contains several phospho-tyrosine binding pockets at the N-terminal end, a SH2 superfamily domain and a SOCS-SOCS3 family domain near the C-terminal end called SOCS box.

General readings

1. Barclay JL, Anderson ST, Waters MJ, Curlewis JD. SOCS3 as a tumor suppressor in breast cancer cells, and its regulation by PRL. Int J Cancer. 2009 Apr 15;124:1756-66.
2. Croker, B.A., D.L. Krebs, J.G. Zhang, S. Wormald, T.A. Willson, E.G. Stanley, L. Robb, C.J. Greenhalgh, I. Forster, B.E. Clausen, et al. 2003. SOCS3 negatively regulates IL-6 signaling in vivo. Nat. Immunol. 4:540–545.
3. Alexander, W.S., & D.J. Hilton. 2004. The role of suppressors of cytokine signaling (SOCS) proteins in regulation of the immune response. Annu. Rev. Immunol. 22:503–529.
4. Gillian M. Tannahill, Joanne Elliott, Anna C. Barry, Linda Hibbert, Nicolas A. Cacalano, and James A. Johnston et. al., SOCS2 Can Enhance Interleukin-2 (IL-2) and IL-3
Signaling by Accelerating SOCS3 Degradation. Mol Cell Biol. 2005 October; 25(20): 9115–9126.
5. J. Barker,10 N. J. Reynolds,11 S. J. Meggitt,12 J. Kere,6 C.-F. Wahlgren,3 G. Pershagen,4,8 M. Wickman,4,5,8 M. Nordenskjöld,1 I. Kockum,1,7 and M. Bradley. Elevated Expression and Genetic Association Links the SOCS3 Gene to Atopic Dermatitis. Am J Hum Genet. 2006 June; 78(6): 1060–1065.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SOCS3 / CIS3 antibody

Figure 1. Western Blotting analysis (Reducing Conditions) of whole cell lysate of HeLa Human cervix carcinoma cell line. 
Lane 1: Immunostaining with Isotype Mouse IgG2b Control (PLRV219, AM03110PU-N). 
Lane 2: Immunostaining with anti-SOCS3 antibody (SO1). Mouse IgG2b SO1 Purified Hu E, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

SOCS3 / CIS3 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502921) Mouse IgG2b OTI5D4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Anti-SOCS3 mouse monoclonal antibody (TA502959) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305 ). Mouse IgG1 OTI11C5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502991) Mouse IgG2a OTI3D3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503039) Mouse IgG2a OTI8G3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503054) Mouse IgG2b OTI15F9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Anti-SOCS3 mouse monoclonal antibody (TA503055) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305 ). Mouse IgG2b OTI3B8 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SOCS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503057) Mouse IgG1 OTI7E10 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 (C-term) antibody

Figure 1. AP15781PU-N SOCS3 antibody staining of Human Placenta. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

SOCS3 / CIS3 (C-term) antibody

Figure 1. AP15781PU-N SOCS3 antibody staining of Human Placenta. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

SOCS3 / CIS3 (C-term) antibody

Figure 1. AP15781PU-N SOCS3 antibody staining of Human Placenta. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

SOCS3 / CIS3 (C-term) antibody

TA302617 staining (5µg/ml) of MOLT-4 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SOCS3 / CIS3 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SOCS3 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SOCS3 / CIS3 antibody

Predicted cell location: Cytoplasm. Positive control: Human ovarian and cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SOCS3 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SOCS3 / CIS3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (RC209305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3. Mouse IgG1 OTI6F3 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Anti-SOCS3 mouse monoclonal antibody (TA502990) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305 ). Mouse IgG1 OTI6C12 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Anti-SOCS3 mouse monoclonal antibody (TA503025) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305). Mouse IgG2a OTI6C2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 antibody

Anti-SOCS3 mouse monoclonal antibody (TA503043) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3(RC209305). Mouse IgG2a OTI5C2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SOCS3 / CIS3 (2-44) antibody

Western blot analysis of GST-SOCS-3 (2-44 a.a.) using AM26562AF-N. Mouse IgG1 1B2 Azide Free Ms IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

SOCS3 / CIS3 (2-44) antibody

SOCS3 / CIS3 Mouse IgG1 1B2 Biotin Ms WB
50 Tests / €390.00
  Acris Antibodies GmbH

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