SOCS4 antibody

Principal name

SOCS4 antibody

Alternative names for SOCS4 antibody

Suppressor of cytokine signaling 4, SOCS-4, SOCS7, Suppressor of cytokine signaling 7, SOCS-7

SwissProt ID

Q0VC91 (Bovin), Q8WXH5 (Human), Q91ZA6 (Mouse)

Gene ID

122809 (SOCS4)

Available reactivities

Hu (Human), Bov (Bovine), Por (Porcine), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Dot blot (Dot), Flow Cytometry (F)

Background of SOCS4 antibody

Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes. SOCS6 and SOCS7 are expressed ubiquitously in murine tissues and SOCS6 knockout mice are growth retarded.

General readings

1. Gregorieff A. et al. J. Biol. Chem. 275(28), 21596(2000).
2. Krebs DL. et al. J. Cell Sci. 113, 2813(2000).
3. Ram PA. et al. J. Biol. Chem. 274(50), 35553(1999).
4. Hilton DJ. et al. Proc. Natl. Acad. Sci USA 95, 114(1998).
5. Yoshimura A. el al. Cytokine Growth Factor Rev 9, 197(1998).
6. Krebs DL. et al. Stem Cells 19, 378(2001)
7. Masuhara M. et al. Biochem Biophys Res Commun
239(2), 439(1997).
8. Minamoto S. et al. Biochem Biophys Res Commun 237, 79(1997).
9. Cohney SJ. et al. Mol. Cell Biol. 19, 4980(1999).
10. Davey HW. et al. Mol. Cwll Endocrinol 158, 111(1999).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SOCS4 antibody

Sample(30 ug of whole cell lysate). A:HeLa S3. 12% SDS PAGE. TA308763 diluted at 1:200 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SOCS4 antibody

Western blot analysis of lysate from MCF-7 cell line,using SOCS4 Antibody (Center)(Cat. #TA324438).TA324438 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SOCS4 (C-term) antibody

SOCS4 antibody Cat.-No AP07230PU-N staining of Formalin-Fixed Paraffin-Embedded Human Adrenal at 10 µg/ml after heat-induced antigen retrieval. Rabbit Aff - Purified Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SOCS4 (C-term) antibody

SOCS4 antibody Cat.-No AP15982PU-N staining of paraffin embedded Human Pancreas at3.8 μg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Hu, Por E, P
0.1 mg / €340.00
  Acris Antibodies GmbH

SOCS4 antibody

Immunofluorescence of purified MaxPab antibody to SOCS4 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SOCS4 antibody

Western Blot analysis of SOCS4 expression in transfected 293T cell line (H00122809-T02) by SOCS4 MaxPab polyclonal antibody.

Lane 1: SOCS4 transfected lysate(50.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SOCS4 antibody

Western Blot analysis of SOCS4 expression in transfected 293T cell line by SOCS4 monoclonal antibody (M01), clone 2G8.

Lane 1: SOCS4 transfected lysate(50.6 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 2G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SOCS4 antibody

SOCS4 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

SOCS4 antibody

The tissue/cell lysates derived from mouse brain ( lane 1 ), HeLa ( lane 2 ) were immuno-probed by SOCS4 polyclonal antibody ( Cat # PAB12781 ) at 1 : 500. An immune-reactive band is observed around ~50 kDa. Rabbit Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SOCS4 antibody

SOCS4 Goat IgG Aff - Purified Hu E, P
0.1 mg / €400.00
  Novus Biologicals Inc.

SOCS4 antibody

SOCS4 Rabbit Aff - Purified Hu IP, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

SOCS4 antibody

Western Blot: SOCS4 Antibody [NBP1-31007] - Western blot analysis of SOCS4 Antibody on: A:HeLa S3(GTX14654) 12% SDS PAGE Diluted at 1:200 Rabbit IgG Hu Dot, ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SOCS4 (full length, N-term HIS tag, transcript variant 1)

SOCS4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

SOCS4 (full length, N-term HIS tag, transcript variant 2)

SOCS4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

SOCS4

SOCS4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SOCS4

SOCS4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SOCS4 293T Cell Transient Overexpression Lysate(Denatured)

SOCS4 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

17 Item(s)

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