Sonic hedgehog (SHH) antibody

Principal name

Sonic hedgehog (SHH) antibody

Alternative names for Sonic hedgehog (SHH) antibody

Sonic hedgehog protein, HHG-1

SwissProt ID

Q15465 (Human), Q62226 (Mouse), Q63673 (Rat), Q91035 (Chick), Q92000 (Xenla), Q92008 (Danre)

Gene ID

20423 (Shh), 6469 (SHH)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Prim (Primate)

Available hosts

Goat, Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT)

Background of Sonic hedgehog (SHH) antibody

SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

General readings

Richieri-Costa A., et al. (2006) Am. J. Med. Genet. A 140:2587-2593.El-Jaick K.B., et al (2005) Am. J. Med. Genet. A 136:345-345.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Sonic hedgehog (SHH) antibody

Western Blot: Sonic Hedgehog Antibody (5H4) [TA336923] - Western blot analysis using Sonic Hedgehog mAb against HEK293 (1) and Sonic Hedgehog (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 5H4 Purified Hu, Ms, Prim E, F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Sonic hedgehog (SHH) antibody

Anti-SHH mouse monoclonal antibody (TA500041) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHH(RC222175). Mouse IgG1 OTI10H6 Purified Can, Hu, Mky, Rt ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Sonic hedgehog (SHH) antibody

Immunohistochemical staining of human kidney shows cytoplasmic and membranous positivity in cells of renal tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Sonic hedgehog (SHH) antibody

Human lung stained with Anti-SHH antibody. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

Sonic hedgehog (SHH) antibody

Human lung stained with Anti-SHH antibody. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

Sonic hedgehog (SHH) antibody

Human lung stained with Anti-SHH antibody. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

Sonic hedgehog (SHH) antibody

Predicted cell location: Cytoplasm . Positive control: Human breast and colon cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SHH antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Sonic hedgehog (SHH) antibody

Predicted cell location: Cytoplasm . Positive control: Human breast and ovarian cancer tissue. Recommended dilution: 1/50-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SHH antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Sonic hedgehog (SHH) antibody

WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Sonic hedgehog (SHH) antibody

Western Blot: Sonic Hedgehog Antibody [TA336926] - WB detection of Sonic Hedgehog in A. human stomach, B. human fetal liver membrane, C. PC12 and D. Cos7 Rabbit Purified Hu, Ms, Prim, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Sonic hedgehog (SHH) antibody

Anti-SHH mouse monoclonal antibody (TA500040) immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH(RC222175). Mouse IgG2a OTI3A2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Sonic hedgehog (SHH) antibody

Western blot analysis using SHH mAb against SHH (AA: 26-161)-hIgGFc transfected HEK293 cell lysate. Mouse IgG1 8G3 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Sonic hedgehog (SHH) (N-term) antibody

Sonic hedgehog (SHH) Rat IgG2 6K12 Purified Ms C, NEUT, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Sonic hedgehog (SHH) (N-term) antibody

Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining SHH in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Sonic hedgehog (SHH) (C-term) antibody

Sonic hedgehog (SHH) Rat IgG2 4A37 Purified Ms C, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Sonic hedgehog (SHH) antibody

Sonic hedgehog (SHH) Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Sonic hedgehog (SHH) antibody

Sonic hedgehog (SHH) Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Sonic hedgehog (SHH) antibody

Detection limit for recombinant GST tagged SHH is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 1B11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Sonic hedgehog (SHH) antibody

Immunohistochemical staining of SHH on formalin fixed, paraffin embedded human lung with SHH polyclonal antibody. Rabbit Hu P
0.1 ml / €390.00
  Abnova Taiwan Corp.

Sonic hedgehog (SHH) antibody

Human glioma; . Sample Type :. Human glioma cells. Primary Antibody Dilution :. 1:200. Secondary Antibody :. Anti-rabbit-GFP. Secondary Antibody Dilution :. 1:500. Color/Signal Descriptions :. 1. SHH: Green 2. DAPI: Blue 3. Merge. Gene Name :. SHH. Submitted by :. ELIAS EL HABR, PhD, Post-Doctoral fellow, INSERM U894 Glial Plasticity Team, Psychiatry& Neurosciences Center, St Anne Hospital, 2 Ter, Rue d'Alesia, 75014 Paris; SHH antibody - N-terminal region (ARP44235_P050) in Human glioma cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

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