SORD antibody

Principal name

SORD antibody

Alternative names for SORD antibody

Sorbitol dehydrogenase, L-iditol 2-dehydrogenase

SwissProt ID

P07846 (Sheep), P27867 (Rat), P36624 (Schpo), Q00796 (Human), Q29318 (Pig), Q58D31 (Bovin), Q64442 (Mouse)

Gene ID

6652 (SORD)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Sh (Sheep)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Frozen Sections (C), Flow Cytometry (F)

Background of SORD antibody

Sorbitol dehydrogenase (SDH), a member of the medium-chain dehydrogenase/reductase protein family and the second enzyme of the polyol pathway of glucose metabolism, converts sorbitol to fructose strictly using NAD(+) as coenzyme. Sorbitol dehydrogenase catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor. It has been noted that the polyol pathway is particularly active in hyperglycemic states. Although SORD is closely related to the class I long-chain alcohol dehydrogenases, it differs in substrate specificity, catalyzing polyols such as sorbitol and xylitol but having no activity towards primary alcohols.

General readings

Karacaoglan V & Ozer I Steady-state kinetic properties of sorbitol dehydrogenase from chicken liver. Comp Biochem Physiol B Biochem Mol Biol 140:309-12 (2005). PubMed: 15649778

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SORD antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-SORDmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500677, Dilution 1:50) Mouse IgG1 OTI12A7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SORDmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500683, Dilution 1:50) Mouse IgG2b OTI2A10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-SORDmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500701, Dilution 1:50) Mouse IgG2a OTI9E7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

SORD Mouse IgG2a OTI1D5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-SORDmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500747, Dilution 1:50) Mouse IgG1 OTI15H2 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

Immunofluorescent staining of HepG2 cells using anti-SORD mouse monoclonal antibody (TA500748). Mouse IgG1 OTI17E7 Purified Hu F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

Anti-SORD mouse monoclonal antibody (TA500785) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SORD(RC200415). Mouse IgG1 OTI9G9 Purified Hu F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

Immunoperoxidase of purified MaxPab antibody to SORD on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SORD antibody

Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml] Mouse IgG1 4D3 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SORD (C-term) antibody

TA302618 (0.1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Chk, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SORD antibody

Predicted band size: 38 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SORD antibody

Western blot analysis of extracts of various cell lines, using SORD antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SORD antibody

Anti-SORD mouse monoclonal antibody (TA500749) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SORD(RC200415). Mouse IgG3 OTI4E5 Purified Hu F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-SORDmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500784, Dilution 1:50) Mouse IgG1 OTI13G5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SORD antibody

The cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SORD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT10F4 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

SORD antibody

The cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SORD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT10F4 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

SORD antibody

SORD Rabbit IgG Aff - Purified Sh Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

SORD (Center) antibody

Western blot analysis of SORD Antibody (Center) Cat.-No AP53985PU-N in Hela cell line lysates (35ug/lane).This demonstrates the SORD antibody detected the SORD protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SORD antibody

SORD polyclonal antibody (A01), Lot # 051123JC01 Western Blot analysis of SORD expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SORD antibody

Western Blot analysis of SORD expression in transfected 293T cell line (H00006652-T01) by SORD MaxPab polyclonal antibody.

Lane 1: SORD transfected lysate(38.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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