SP1 / TSFP1 antibody

Principal name

SP1 / TSFP1 antibody

Alternative names for SP1 / TSFP1 antibody

Transcription factor Sp1

SwissProt ID

O89090 (Mouse), P08047 (Human), P81121 (Horse), Q01714 (Rat)

Gene ID

6667 (SP1), 20683 (Sp1), 2666723 (accA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Chk (Chicken), African clawed frog, Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ChIP assay (ChIP)

Background of SP1 / TSFP1 antibody

The sequence-specific transcription factor Sp1 binds GC box sequences in the promoters of a variety of genes. The 97 kD Sp1 transcription factor contains two zinc finger domains that determine sequence specificity of binding to the 5'-GGGGCGGGGC-3'. This transcription factor regulates nucleoside, nucleotide and nucleic acid metabolism and many other "housekeeping" genes. Sp1 can be modified by phosphorylation by the upstream kinase ERK1 and has been shown to interact with a large number of proteins. The transcriptional activity of Sp1 has been shown to be disrupted in early Huntington's disease. 
Structure: Transcription factor interacting with promoters containing GC-box elements, contains 2 zinc finger domains and requires zinc for sequence-specific DNA binding, approximately 97 kD.
Distribution: Ubiquitous nuclear expressionFunction:Transcription factor regulating nucleoside, nucleotide and nucleic acid metabolism as well as many other "housekeeping" genes.
Modification: Phosphorylation (Ser 59, Thr 350, Thr736).
Interaction: ELF1 transcription factor, myogenic factor 4, Shc, TAFII130, E2F, histone deacetylase 1, Sp3, c-Jun, SMAD4, SMAD2, BRCA1, c-Myc, and many others.

General readings

Sakuntabhai A, Turbpaiboon C, Casademont I, Chuansumrit A, Lowhnoo T, Kajaste-Rudnitski A, Kalayanarooj SM, Tangnararatchakit K, Tangthawornchaikul N, Vasanawathana S, Chaiyaratana W, Yenchitsomanus PT, Suriyaphol P, Avirutnan P, Chokephaibulkit K, Matsuda F, Yoksan S, Jacob Y, Lathrop GM, Malasit P, Despres P, Julier C. A variant in the CD209 promoter is associated with severity of dengue disease. Nat Genet. 2005 May;37(5):507-13. Epub 2005 Apr 17.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SP1 / TSFP1 (C-term) antibody

Immunohistochemistry analysis in human colon carcinoma (Formalin-fixed, Paraffin-embedded) using SP1 / TSFP1 antibody Cat.-No. AP53987PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SP1 / TSFP1 antibody for IHC; Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SP1 / TSFP1 antibody

Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml] Mouse IgG1 1A5 Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP1 / TSFP1 antibody

Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG1 4C8 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP1 / TSFP1 antibody

Western Blot analysis of SP1 expression in transfected 293T cell line by SP1 monoclonal antibody (M05), clone 4B11.

Lane 1: SP1 transfected lysate(80.693 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4B11 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP1 / TSFP1 (609-627) antibody

AP15792PU SP1 antibody staining of Human Tonsil. Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

SP1 / TSFP1 (609-627) antibody

AP15792PU SP1 antibody staining of Human Tonsil. Rabbit Purified Hu, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

SP1 / TSFP1 (609-627) antibody

AP15792PU SP1 antibody staining of Human Tonsil. Rabbit Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

SP1 / TSFP1 antibody

TA303043 staining (0.3µg/ml) of Hela lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

SP1 / TSFP1 antibody

TA303043 staining (0.3µg/ml) of Hela lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SP1 / TSFP1 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and ovarian cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using SP1 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SP1 / TSFP1 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and ovarian cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using SP1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SP1 / TSFP1 antibody

Western blot analysis of SP1 expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SP1 / TSFP1 antibody

Western blot analysis of SP1 expression in HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SP1 / TSFP1 antibody

Western blot (WB) analysis of SP1 antibody (Cat.-No.: AP06584PU-N) in K-562 (A) and A-431 (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SP1 / TSFP1 pThr739 antibody

Western blot (WB) analysis of p-Sp1 antibody (Cat.-No.: AP20842PU-N) in extracts from COS serum 15' cells Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SP1 / TSFP1 antibody

Formalin-Fixed Paraffin-Embedded Human breast tissue stained with SP1 Antibody Cat.-No AP33175PU and developed with an Alkaline Phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.5 ml / €390.00
  Acris Antibodies GmbH

SP1 / TSFP1 antibody

Formalin-Fixed Paraffin-Embedded Human breast tissue stained with SP1 Antibody Cat.-No AP33175PU and developed with an Alkaline Phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.125 ml / €210.00
  Acris Antibodies GmbH

SP1 / TSFP1 antibody

Western blot analysis of HeLa nuclear extract probed with RABBIT ANTI HUMAN SP1
(SP1154P) followed by SHEEP ANTI RABBIT IgG:HRP (SP2041HRP). Rabbit Purified Hu IP, WB
0.1 mg / €520.00
  Acris Antibodies GmbH

SP1 / TSFP1 antibody

SP1 / TSFP1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SP1 / TSFP1 antibody

SP1 monoclonal antibody (M08), clone 2G5. Western Blot analysis of SP1 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 2G5 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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