SP3 / SPR-2 antibody

Principal name

SP3 / SPR-2 antibody

Alternative names for SP3 / SPR-2 antibody

Transcription factor Sp3

SwissProt ID

O70494 (Mouse), Q02447 (Human), Q90WR8 (Chick)

Gene ID

6670 (SP3), 20687 (Sp3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Ze (Zebrafish), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of SP3 / SPR-2 antibody

The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3,but not Sp2 or Sp4, interact with E2, a regulatory element for the β4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene encoding human Sp2 maps to chromosome 17p32.3.

General readings

Whitten,C., Gene 413 (1-2), 58-66 (2008)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SP3/4 antibody

Immunohistochemistry (IHC) analysis of Sp3/4 antibody (Cat.-No.: AP06710PU-N) on paraffin-embedded sections. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SP3/4 antibody

Immunofluorescent analysis of SP3/4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Chk, Hu, Ms, Ze ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SP3/4 antibody

Immunofluorescent analysis of SP3/4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Chk, Hu, Ms, Ze ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SP3/4 antibody

Immunofluorescent analysis of SP3/4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Chk, Hu, Ms, Ze ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SP3 / SPR-2 antibody

Western blot analysis of extracts from Jurkat cells, using SP3/4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SP3 / SPR-2 antibody

Western blot analysis of Sp3 using lysates from Jurkat cells (Lane 1& 2), 3T3 cells (Lane 3) and Rat kidney (Lane 4). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SP3 / SPR-2 antibody

Staining of mouse bone marrow with Anti-Mouse CD202b (TIE2) PE and Anti-Mouse Ly-6A/E (Sca-1) APC + Anti-Mouse CD117 (c-Kit) APC (cat 17-1171).  Total viable lineage negative cells were used for analysis. Rat TEK4 Purified Ms F, P
50 µg / €120.00
  OriGene Technologies, Inc.

SP3 / SPR-2 antibody

WB Suggested Anti-SP3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SP3 / SPR-2 (C-term) antibody

Formalin-Fixed Paraffin-Embedded Human tonsil tissue was stained with SP3 Antibody Cat.-No AP33176PU and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.2 ml / €390.00
  Acris Antibodies GmbH

SP3 / SPR-2 (C-term) antibody

Formalin-Fixed Paraffin-Embedded Human tonsil tissue was stained with SP3 Antibody Cat.-No AP33176PU and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µl / €230.00
  Acris Antibodies GmbH

SP3 / SPR-2 antibody

SP3 / SPR-2 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 / SPR-2 Mouse IgG2a 3D4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 monoclonal antibody (M07), clone 2E5 Western Blot analysis of SP3 expression in A-549 ( Cat # L025V1 ). Mouse IgG2a 2E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 monoclonal antibody (M08), clone 2F6. Western Blot analysis of SP3 expression in human stomach. Mouse IgG2a 2F6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 monoclonal antibody (M09), clone 4E5. Western Blot analysis of SP3 expression in human stomach. Mouse IgG2a 4E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 / SPR-2 Mouse IgG2a 2E8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 / SPR-2 Mouse IgG2a 2D7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 monoclonal antibody (M12), clone 3G5. Western Blot analysis of SP3 expression in human stomach. Mouse IgG2a 3G5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 monoclonal antibody (M13), clone 1D3. Western Blot analysis of SP3 expression in human Intestinal wall. Mouse IgG2a 1D3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SP3 / SPR-2 antibody

SP3 / SPR-2 Mouse IgG2a 4B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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