SPAG1 antibody

Principal name

SPAG1 antibody

Alternative names for SPAG1 antibody

Sperm-associated antigen 1, SPAG-1, HSD-3.8

SwissProt ID

Q07617 (Human), Q5U2X2 (Rat), Q80ZX8 (Mouse)

Gene ID

6674 (SPAG1), 26942 (Spag1), 315033

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of SPAG1 antibody

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility; as well as identification of proteins for targeted contraception; are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutiting antibodies from an infertile woman. Furthermore; immunization of female rats with the recombint human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombint polypeptide binds GTP and exhibits GTPase activity. Thus; this protein may regulate GTP sigl transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPAG1 antibody

  Anti-SPAG1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human lung and ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lung and ovarian cancer tissue using SPAG1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG1 antibody

  Anti-SPAG1 Rabbit Polyclonal Antibody  
Predicted band size: 104 kDa . Positive control: Human colon tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG1 antibody

  Rabbit Polyclonal Anti-SPAG1 Antibody  
Host: Rabbit; Target Name: SPAG1; Sample Tissue: MCF7 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPAG1 antibody

  Rabbit Polyclonal Anti-SPAG1 Antibody  
WB Suggested Anti-SPAG1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal kidney Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

SPAG1 - human gene knockout kit via CRISPR

SPAG1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SPAG1 - human gene knockout kit via CRISPR

SPAG1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SPAG1 - human gene knockout kit via CRISPR

SPAG1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SPAG1 - human gene knockout kit via CRISPR

SPAG1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Spag1 - mouse gene knockout kit via CRISPR

Spag1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Spag1 - mouse gene knockout kit via CRISPR

Spag1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Spag1 - mouse gene knockout kit via CRISPR

Spag1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Spag1 - mouse gene knockout kit via CRISPR

Spag1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPAG1 (transcript variant 1)

  Recombinant protein of human sperm associated antigen 1 (SPAG1), transcript variant 1  
SPAG1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SPAG1 overexpression lysate

SPAG1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SPAG1 (mGFP-tagged)-Human sperm associated antigen 1 (SPAG1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SPAG1 (mGFP-tagged)-Human sperm associated antigen 1 (SPAG1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,930.00
  OriGene Technologies, Inc.

Lenti ORF particles, SPAG1 (Myc-DDK-tagged)-Human sperm associated antigen 1 (SPAG1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SPAG1 (Myc-DDK-tagged)-Human sperm associated antigen 1 (SPAG1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,740.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Spag1 (GFP-tagged) - Mouse sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Spag1 (GFP-tagged) - Mouse sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL  
200 µl / €1,650.00
  OriGene Technologies, Inc.

Lenti ORF particles, Spag1 (Myc-DDK-tagged) - Mouse sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Spag1 (Myc-DDK-tagged) - Mouse sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL  
200 µl / €1,480.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Spag1 (GFP-tagged ORF) - Rat sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Spag1 (GFP-tagged ORF) - Rat sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL  
200 µl / €1,640.00
  OriGene Technologies, Inc.

Lenti ORF particles, Spag1 (Myc-DDK-tagged ORF) - Rat sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Spag1 (Myc-DDK-tagged ORF) - Rat sperm associated antigen 1 (Spag1), 200 uL, >10^7 TU/mL  
200 µl / €1,473.00
  OriGene Technologies, Inc.

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