SPAG4 / SUN4 antibody

Principal name

SPAG4 / SUN4 antibody

Alternative names for SPAG4 / SUN4 antibody

Sperm-associated antigen 4 protein, Outer dense fiber-associated protein SPAG4, SUN domain-containing protein 4

SwissProt ID

O55034 (Rat), Q9JJF2 (Mouse), Q9NPE6 (Human)

Gene ID

6676 (SPAG4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SPAG4 / SUN4 antibody

The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology; reduced sperm motility; and infertility. In the rat; the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPAG4 / SUN4 antibody

Predicted band size: 48 kDa . Positive control: Hela and K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane1: K562 cell lysate Lysate: 40 ug per lane Primary antibody: 1/1100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG4 / SUN4 antibody

Predicted band size: 48 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG4 / SUN4 antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and lung cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SPAG4 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG4 / SUN4 antibody

Predicted band size: 48 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/100-500. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG4 / SUN4 antibody

SPAG4 / SUN4 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

SPAG4 / SUN4 antibody

SPAG4 / SUN4 Mouse IgG1 3C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPAG4 / SUN4

SPAG4 / SUN4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SPAG4 / SUN4

SPAG4 / SUN4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SPAG4 overexpression lysate

SPAG4 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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