SPAG5 / MAP126 antibody

Principal name

SPAG5 / MAP126 antibody

Alternative names for SPAG5 / MAP126 antibody

Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126

SwissProt ID

Q7TME2 (Mouse), Q96R06 (Human)

Gene ID

10615 (SPAG5)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rb (Rabbit), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SPAG5 / MAP126 antibody

Sperm-associated antigen 5 is a protein that in humans is encoded by the SPAG5 gene. This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functiol and dymic regulation of mitotic spindles.?Essential component of the mitotic spindle required for normal chromosome segregation and progression into aphase. Required for chromosome alignment; normal timing of sister chromatid segregation; and maintence of spindle pole architecture. The astrin (SPAG5)-kistrin (SKAP) complex promotes stable microtubule-kinetochore attachments.

General readings

Chang M.S. et al. Biochem Biophys Res Commun 2001 Sep 14;287(1):116-21.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPAG5 / MAP126 antibody

Predicted cell location: Cytoplasm . Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SPAG5 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG5 / MAP126 antibody

Predicted cell location: Cytoplasm . Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SPAG5 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG5 / MAP126 antibody

SPAG5 monoclonal antibody (M01), clone 5F9 Western Blot analysis of SPAG5 expression in HeLa ( Cat # L013V1 ). Mouse IgG3 5F9 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SPAG5 / MAP126 antibody

SPAG5 monoclonal antibody (M01), clone 5F9 Western Blot analysis of SPAG5 expression in HeLa ( Cat # L013V1 ). Mouse IgG3 5F9 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

SPAG5 / MAP126 antibody

SPAG5 / MAP126 Rabbit Hu WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

SPAG5 / MAP126 antibody

Western Blot: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3  mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another  rabbit anti-Astrin/MAP126 antibody and NB100-74639,  which recognize downstream epitopes. Detection: Chemiluminescence  with an exposure time of 30 seconds. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

SPAG5 / MAP126 antibody

Western Blot: Astrin Antibody [NB100-74639] - Detection of Human Astrin/MAP126 by Western Blot and  Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50  mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody  NB100-74639 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B)  and used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also  immunoprecipitated by another  rabbit anti-Astrin/MAP126 antibody and NB100-74638 , which recognize upstream epitopes.  Detection: Chemiluminescence with exposure times of 30  seconds (A and B). Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SPAG5 / MAP126 antibody

SPAG5 / MAP126 Rabbit Aff - Purified Hu WB
0.1 mg / €320.00
  Novus Biologicals Inc.

SPAG5 / MAP126 antibody

Immunohistochemistry-Paraffin: Astrin Antibody [NBP1-85261] - Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

SPAG5 / MAP126 antibody

Immunohistochemistry-Paraffin: Astrin Antibody [NBP1-85262] - staining of human small intestine shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPAG5 / MAP126

SPAG5 / MAP126 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SPAG5 / MAP126

SPAG5 / MAP126 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SPAG5 / MAP126

SPAG5 / MAP126 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SPAG5 overexpression lysate

SPAG5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

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