SPAG6 / PF16 antibody

Principal name

SPAG6 / PF16 antibody

Alternative names for SPAG6 / PF16 antibody

Sperm-associated antigen 6, Sperm flagellar protein, Repro-SA-1

SwissProt ID

O75602 (Human), Q9JLI7 (Mouse)

Gene ID

9576 (SPAG6), 50525 (Spag6)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of SPAG6 / PF16 antibody

SPAG6 is important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintence of the structural integrity of mature sperm. Altertively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPAG6 / PF16 antibody

WB Suggested Anti-SPAG6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPAG6 / PF16 antibody

WB Suggested Anti-SPAG6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPAG6 / PF16 antibody

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human testis tissue, Primary antibody: (SPAG6 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG6 / PF16 antibody

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (SPAG6 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG6 / PF16 antibody

Western Blot analysis of SPAG6 expression in transfected 293T cell line (H00009576-T01) by SPAG6 MaxPab polyclonal antibody.

Lane 1: SPAG6 transfected lysate(55.99 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SPAG6 / PF16 antibody

Western Blot analysis of SPAG6 expression in transfected 293T cell line (H00009576-T01) by SPAG6 MaxPab polyclonal antibody.

Lane 1: SPAG6 transfected lysate(55.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SPAG6 / PF16 antibody

SPAG6 monoclonal antibody (M01), clone 3E3 Western Blot analysis of SPAG6 expression in A-431 ( Cat # L015V1 ). Mouse IgG3 3E3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SPAG6 / PF16 antibody

Detection limit for recombinant GST tagged SPAG6 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 2F3 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SPAG6 / PF16 antibody

Human HT1080; WB Suggested Anti-SPAG6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HT1080 cell lysate; SPAG6 antibody - middle region (ARP53681_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SPAG6 / PF16 antibody

SPAG6 / PF16 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Spag6 - mouse gene knockout kit via CRISPR

Spag6 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Spag6 - mouse gene knockout kit via CRISPR

Spag6 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Spag6 - mouse gene knockout kit via CRISPR

Spag6 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Spag6 - mouse gene knockout kit via CRISPR

Spag6 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPAG6 / PF16 (transcript variant 2)

SPAG6 / PF16 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

SPAG6 / PF16

SPAG6 / PF16 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SPAG6 293T Cell Transient Overexpression Lysate(Denatured)

SPAG6 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

SPAG6 overexpression lysate

SPAG6 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

SPAG6 overexpression lysate

SPAG6 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SPAG6 (mGFP-tagged)-Human sperm associated antigen 6 (SPAG6), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, SPAG6 (mGFP-tagged)-Human sperm associated antigen 6 (SPAG6), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,140.00
  OriGene Technologies, Inc.

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