SPAG8 / SMP1 antibody

Principal name

SPAG8 / SMP1 antibody

Alternative names for SPAG8 / SMP1 antibody

Sperm-associated antigen 8, SMP-1, Sperm membrane protein 1, BS-84, HSD-1

SwissProt ID

Q99932 (Human), Q99932-2

Gene ID

26206 (SPAG8)

Available reactivities

Hu (Human), Ms (Mouse), Dros (Drosophila), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of SPAG8 / SMP1 antibody

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. SPAG8 is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

General readings

Cheng,G.Y., (2007) Asian J. Androl. 9 (1), 23-29

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPAG8 / SMP1 antibody

Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using SPAG8 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG8 / SMP1 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using SPAG8 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPAG8 / SMP1 antibody

WB Suggested Anti-SPAG8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Hu, Rb, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPAG8 / SMP1 antibody

WB Suggested Anti-SPAG8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPAG8 / SMP1 (C-term) antibody

Western blot analysis of SPAG8 / SMP1 Antibody Cat.-No  AP53999PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the SPAG8 antibody detected the SPAG8 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SPAG8 / SMP1 antibody

SPAG8 MaxPab polyclonal antibody. Western Blot analysis of SPAG8 expression in human kidney. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SPAG8 / SMP1 antibody

SPAG8 MaxPab polyclonal antibody. Western Blot analysis of SPAG8 expression in human kidney. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

SPAG8 / SMP1 antibody

Western Blot analysis of SPAG8 expression in transfected 293T cell line by SPAG8 monoclonal antibody (M01), clone 2F12.

Lane 1: SPAG8 transfected lysate(53.5 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2F12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SPAG8 / SMP1 antibody

Human Liver; WB Suggested Anti-SPAG8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; SPAG8 antibody - N-terminal region (ARP55621_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Dros, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

SPAG8 / SMP1 antibody

Human HeLa; WB Suggested Anti-SPAG8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; SPAG8 antibody - middle region (ARP55622_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SPAG8 / SMP1 antibody

Western Blot: SPAG8 Antibody [NBP1-54628] - This Anti-SPAG8 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPAG8 / SMP1

SPAG8 / SMP1 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

SPAG8 / SMP1

SPAG8 / SMP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SPAG8 / SMP1

SPAG8 / SMP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SPAG8 293T Cell Transient Overexpression Lysate(Denatured)

SPAG8 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

SPAG8 overexpression lysate

SPAG8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

per page
  • LinkedIn