SPAG9 / JIP4 antibody

Principal name

SPAG9 / JIP4 antibody

Alternative names for SPAG9 / JIP4 antibody

Sperm-associated antigen 9, Sperm-specific protein, Sperm surface protein, JNK-interacting protein 4, JIP-4, HSS, KIAA0516, MAPK8IP4, SYD1, HLC6, HLC-6, PHET, CT89, Sunday driver 1, C-jun-amino-terminal kinase-interacting protein 4, JNK-associated leucine-zipper protein, Mitogen-activated protein kinase 8-interacting protein 4, Human lung cancer oncogene 6 protein, Proliferation-inducing protein 6, Protein highly expressed in testis, Cancer/testis antigen 89

SwissProt ID

O60271 (Human), Q03361 (Yeast), Q58A65 (Mouse), Q8CJC2 (Mouse), Q96II0 (Human)

Gene ID

9043 (SPAG9)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C)

Background of SPAG9 / JIP4 antibody

JIP-4 is a 1,321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPAG9 / JIP4 antibody

SPAG9 / JIP4 Rabbit Aff - Purified Hu IP, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

SPAG9 / JIP4 (N-term) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (N-term) (AP14021PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SPAG9 / JIP4 (Center) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SPAG9 / JIP4 antibody

SPAG9 / JIP4 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SPAG9 / JIP4 antibody

Western blot analysis of SPAG9 polyclonal antibody ( Cat # PAB2861 ) .293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the SPAG9 ( N-term ) gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SPAG9 / JIP4 antibody

SPAG9 / JIP4 Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SPAG9 / JIP4 antibody

SPAG9 / JIP4 Rabbit Hu, Ms IP, WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

SPAG9 / JIP4 antibody

Western blot analysis of JIP4 expression in HEK293T (A); Raw264.7 (B); SP2/0 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SPAG9 / JIP4 antibody

Western blot analysis of JIP4 expression in HEK293T (A); Raw264.7 (B); SP2/0 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SPAG9 / JIP4 antibody

Western blot analysis of JIP4 expression in HEK293T (A); Raw264.7 (B); SP2/0 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SPAG9 / JIP4 antibody

Detection of Human JLP by Western Blot. Samples: Nuclear  extract (NE, 20 mcg) from HeLa cells. Antibody: Affinity  purified rabbit anti-JLP antibody (Cat. No. NB100-79793) used at  0.3 mcg/ml. Detection: Chemiluminescence with an exposure  time of 30 second Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SPAG9 / JIP4 antibody

Detection of Human JLP by Western Blot. Samples: Nuclear  extract (NE, 20 mcg) from HeLa cells. Antibody: Affinity  purified rabbit anti-JLP antibody (Cat. No. NB100-79794) used at  0.3 mcg/ml. Detection: Chemiluminescence with an exposure  time of 30 seconds.  25043 Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SPAG9 / JIP4 antibody

Western Blot: SPAG9 Antibody [NBP1-31153] - Western blot analysis of SPAG9 Antibody on: A: H1299 7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu Dot, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

SPAG9 / JIP4 antibody

Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPAG9 / JIP4 (transcript variant 3)

SPAG9 / JIP4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SPAG9 / JIP4

SPAG9 / JIP4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SPAG9 / JIP4

SPAG9 / JIP4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

17 Item(s)

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