SPARC / Osteonectin antibody

Principal name

SPARC / Osteonectin antibody

Alternative names for SPARC / Osteonectin antibody

ON, Basement-membrane protein 40, BM-40, Secreted protein acidic and rich in cysteine

SwissProt ID

P07214 (Mouse), P09486 (Human), P13213 (Bovin), P16975 (Rat), P20112 (Pig), P36233 (Rabit), P36377 (Chick)

Gene ID

6678 (SPARC), 20692 (Sparc)

Available reactivities

Bov (Bovine), Hu (Human), Rt (Rat), Por (Porcine), Rb (Rabbit), Ms (Mouse), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of SPARC / Osteonectin antibody

SPARC, secreted protein acidic and rich in cysteine , also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I) , 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

General readings

1. Shi Q, et al. "Targeting SPARC expression decreases glioma cellular survival and invasion associated with reduced activities of FAK and ILK kinases." Oncogene. 2007 Jan 8; [Epub ahead of print] PMID: 17213807.
2. Aouacheria A, et al. "In silico whole-genome screening for cancer-related single-nucleotide polymorphisms located in human mRNA untranslated regions."BMC Genomics. 2007 Jan 3;8(1):2 [Epub ahead of print] PMID: 17201911.
3. Xue LY, et al. "Tissue microarray analysis reveals a tight correlation between protein expression pattern and progression of esophageal squamous cell carcinoma."
BMC Cancer. 2006 Dec 22;6(1):296 [Epub ahead of print] PMID: 17187659.
4. Arp HP, et al. "Predicting the partitioning behavior of various highly fluorinated compounds." Environ Sci Technol. 2006 Dec 1;40(23):7298-304. PMID: 17180981.
5. Kelly KA, et al. "SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration." J Leukoc Biol. 2006 Dec 18; [Epub ahead of print].
6. Markovic, S., et al. "Tumor SPARC microenvironment signature (SMS) and plasma levels in a phase II trial of unresectable stage IV melanoma treated with nab-paclitaxel and carboplatin: A translational study of NCCTG trial N057E." J. Clin. Oncol. 2010, Vol. 28, No. 15, 8578
7. Weiss, G.J., et al. "Molecular characterization of interdigitating dendritic cell sarcoma." Rare Tumors, 2010, 2:e50

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (1)
  4. Overnight Delivery (1)

Items 1 to 20 of 42 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPARC / Osteonectin antibody

Immunohistochemistry: Detection of endogenous SPARC in human hepatocellular carcinoma (HCC). 2 µg/ml AP05261PU-N, developed with Vector ImmPress anti-rabbit HRP and DAB substrate. Rabbit Aff - Purified Hu, Ms C, E, WB
70 µg / €480.00
  Acris Antibodies GmbH

SPARC / Osteonectin antibody

SPARC monoclonal antibody (M02), clone 1B2. Western Blot analysis of SPARC expression in human kidney. Mouse IgG1 1B2 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SPARC / Osteonectin antibody

Western blot analysis of extracts of various cell lines , using SPARC antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SPARC / Osteonectin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPARC (RC209964, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPARC. Mouse IgG1 OTI1D6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SPARC / Osteonectin antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SPARC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507359) Mouse IgG1 OTI2B1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SPARC / Osteonectin antibody

Western Blot analysis of Osteonectin isolated from Human bone. Osteonectin was treated with N-glucosidase F or endoglycosidase H, separated by SDS-PAGE and immunobotted with AP02133SU at 1/2000 dilution. This antibody detects glycosylated Osteonectin. Kaufmann B et al. (2004) Glycobiology 7: 609-619. Rabbit Serum Hu E, P, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

SPARC / Osteonectin antibody

Western Blot analysis of Osteonectin isolated from Human bone. Osteonectin was treated with N-glucosidase F or endoglycosidase H, separated by SDS-PAGE and immunobotted with AP02133SU at 1/2000 dilution. This antibody detects glycosylated Osteonectin. Kaufmann B et al. (2004) Glycobiology 7: 609-619. Rabbit Serum Hu E, P, WB
20 µl / €150.00
  Acris Antibodies GmbH

SPARC / Osteonectin (272-286) antibody

SPARC / Osteonectin Rabbit Serum Hu E
0.1 ml / €380.00
  Acris Antibodies GmbH

SPARC / Osteonectin (272-286) antibody

SPARC / Osteonectin Rabbit Serum Hu E
20 µl / €150.00
  Acris Antibodies GmbH

SPARC / Osteonectin Control Peptide

SPARC / Osteonectin Purified
50 µg / €210.00
  Acris Antibodies GmbH

SPARC / Osteonectin antibody

Detection of endogenous SPARC in mouse heart lysate using AP05261SU-N (5 µg/ml) and anti-rabbit HRP (1:75k) and using Pierce's Super Signal West Femto. Rabbit IgG Purified Hu, Ms C, E, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SPARC / Osteonectin (C-term) antibody

Western blot analysis of SPARC Antibody (C-term) (AP17759PU-N) in Y79 cell line lysates (35ug/lane). SPARC (arrow) was detected using the purified Pab. Rabbit Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

SPARC / Osteonectin antibody

SPARC / Osteonectin Mouse IgG1 N50 Azide Free Bov, Hu, Rt C, E, F, ICC/IF, P, R, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

SPARC / Osteonectin antibody

SPARC / Osteonectin Rat IgG2 5F41 Purified Ms C, E
0.1 mg / €440.00
  Acris Antibodies GmbH

SPARC / Osteonectin antibody

Western Blot analysis of SPARC expression in transfected 293T cell line (H00006678-T02) by SPARC MaxPab polyclonal antibody.

Lane 1: SPARC transfected lysate(34.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SPARC / Osteonectin antibody

SPARC monoclonal antibody (M02), clone 1B2. Western Blot analysis of SPARC expression in human kidney. Mouse IgG1 1B2 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

SPARC / Osteonectin antibody

SPARC monoclonal antibody (M04), clone 1A2. Western Blot analysis of SPARC expression in human kidney. Mouse IgG1 1A2 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

SPARC / Osteonectin antibody

SPARC / Osteonectin Mouse IgG1 Hu C, E, R, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SPARC / Osteonectin antibody

SPARC / Osteonectin Mouse IgG1 15G12 Hu P, WB
1 ml / €1,080.00
  Abnova Taiwan Corp.

SPARC / Osteonectin antibody

SPARC / Osteonectin Mouse IgG1 A3A8 Bov, Hu, Rt C, E, R, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

Items 1 to 20 of 42 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn