SPDEF antibody

Principal name

SPDEF antibody

Alternative names for SPDEF antibody

PDEF, PSE, SAM pointed domain-containing Ets transcription factor, Prostate-derived Ets factor, Prostate epithelium-specific Ets transcription factor

SwissProt ID

O95238 (Human), Q9WTP3 (Mouse)

Gene ID

25803 (SPDEF), 30051 (Spdef)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of SPDEF antibody

Kit Component:
- KN205688G1, SPDEF gRNA vector 1 in pCas-Guide vector
- KN205688G2, SPDEF gRNA vector 2 in pCas-Guide vector
- KN205688D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Fowler,M., (2006) J. Cell. Biochem. 97 (1), 1-17

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPDEF antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human tonsil and colon cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using SPDEF antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPDEF antibody

WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPDEF antibody

WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPDEF antibody

WB Suggested Anti-SPDEF Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPDEF antibody

Human MCF7; WB Suggested Anti-SPDEF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate. SPDEF is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SPDEF antibody - N-terminal region (AP42057PU-N) in Human MCF7 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  Acris Antibodies GmbH

SPDEF antibody

SPDEF Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SPDEF antibody

Western Blot analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.

Lane 1: SPDEF transfected lysate(37.518 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 4A5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SPDEF antibody

Human MCF7; WB Suggested Anti-SPDEF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate. SPDEF is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SPDEF antibody - N-terminal region (ARP31841_T100) in Human MCF7 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

SPDEF antibody

Human Placenta; WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta; SPDEF antibody - middle region (ARP32533_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SPDEF antibody

Human HeLa; WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; SPDEF antibody - N-terminal region (ARP32534_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Por WB
50 µg / €350.00
  AVIVA Systems Biology

SPDEF (C-term) antibody

Human Fetal Muscle; WB Suggested Anti-SPDEF Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Muscle; SPDEF antibody - C-terminal region (ARP38992_P050) in Human Fetal Muscle cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

SPDEF - human gene knockout kit via CRISPR

SPDEF - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SPDEF - human gene knockout kit via CRISPR

SPDEF - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SPDEF - human gene knockout kit via CRISPR

SPDEF - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SPDEF - human gene knockout kit via CRISPR

SPDEF - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPDEF

SPDEF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SPDEF

SPDEF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SPDEF

SPDEF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

SPDEF

SPDEF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SPDEF

SPDEF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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