Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish)
Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Spectrin beta II / SPTBN1 antibody
Spectrin is an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha I and spectrin beta I are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta II (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Defects in the SPTB gene encoding spectrin beta I are the cause of spherocytosis type I (SPH1), a disorder characterized by severe hemolytic anemia. Another disorder due to defects of SPTB is called elliptocytosis or elliptocytosis 3 (EL3). The human SPTB gene maps to chromosome 14q22-q23.2 and encodes the 2,137 amino acid, 246 kDa erythroid form of beta-spectrin.
Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NB100-40829] - FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-SPTBN1 used at a dilution of 1:250.
Western Blot and Immunoprecipitation: SPTBN1 Antibody [NB100-40829] - Detection of Human SPTBN1 on HeLa whole cell lysate using NB100-40829. SPTBN1 was also immunoprecipitated by rabbit anti-SPTBN1 antibody NB100-40828.