Sphingosine kinase 1 (SPHK1) antibody

Principal name

Sphingosine kinase 1 (SPHK1) antibody

Alternative names for Sphingosine kinase 1 (SPHK1) antibody

SPHK, SPK, SPK 1, SPK-1

SwissProt ID

Q8CI15 (Mouse), Q91V26 (Rat), Q9NYA1 (Human)

Gene ID

8877 (SPHK1), 170897 (Sphk1), 20698

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Prim (Primate), Can (Canine), Mky (Monkey), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of Sphingosine kinase 1 (SPHK1) antibody

Spingosine Kinase 1 (Sphk1) is a key lipid enzyme of the sphingolipid metabolic pathway and it catalyzes the phosphorylation of sphingosine, converting sphingosine into pro-growth and anti-apoptotic sphingosine 1-phosphate (S1P) (1). S1P plays a major regulatory role in tumor cell growth, survival, invasion, and angiogenesis (2). Sphk1, while producing pro-growth S1P, also decrease intracellular levels of pro-apoptotic ceramide. By regulating intracellular balance between S1P and its metabolic precursors, ceramide and sphingosine, Sphk1 plays a key role in determining whether a cell proliferates or undergoes apoptosis or growth arrest (3). Sphk1 also acts as a key regulator of acute and delayed monocyte activation. Its levels were significantly upregulated in CXC chemokine ligand 4 (CXCL4)-treated monocytes (4). Furthermore, inhibition of Sphk1 resulted in the reduction in monocyte cell survival, cytokine expression, and release of oxygen radicals.
The upregulation of Sphk1 mRNA and its expression was reported to be significantly higher in various tumors. Therefore, Sphk1 may also act as an oncogene (5). Sphk1 was inhibited in prostrate cancer cells and animal models undergoing anti-cancer treatments (6). Sphk1 levels were also up-regulated in stimulated phagocytes of human and mice with severe sepsis. Inhibition of this protein inhibited phagocyte production of endotoxin-induced pro-inflammatory cytokines that contribute to sepsis and also protected mice against sepsis (7). Sphk1 is located on human chromosome 17q25.2. It is highly expressed in adult liver, kidney, heart and skeletal muscle. In rat and mouse, Sphk1 is approximately a 45.9kDa protein (383 amino acids). In humans, it is a 56.4kDa protein (470 amino acids).

General readings

Kase H, Hattori Y, Jojima T, Okayasu T, Tomizawa A, Suzuki K, Banba N, Monden T, Satoh H, Akimoto K, Kasai K. Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells. Life Sci. 2007 Aug 23;81(11):939-43. Epub 2007 Aug 17. PMID: 17822721

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Sphingosine kinase 1 (SPHK1) antibody

Sphingosine kinase 1 (SPHK1) Rabbit IgG Aff - Purified Bov, Hu, Ms, Rt E, WB
10 Blots / €500.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) (Center) antibody

The anti-SPHK1 Pab (Cat. #TA324909) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate. Rabbit Ig Purified Hu, Ms, Prim, Rt P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507215) Mouse IgG2a OTI1A6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Sphingosine kinase 1 (SPHK1) (C-term) antibody

AP15946PU-N SPHK1 antibody staining of Human Lung lysate at 1µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
20 µg / €200.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) antibody

TA305846 (2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) antibody

SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody, which recognizes an upstream epitope Rabbit IgG Purified Hu IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) antibody

Western blot analysis of Sphingosine Kinase 1 in Jurkat cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) antibody

Western blot analysis of extracts of various cell lines, using SPHK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) (N-term) antibody

Anti-SPHK1 antibody IHC of human adrenal, pheochromocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bt, Can, Elephant, Eq, Hu, Mky, Prim, Rb P
50 µg / €379.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) (N-term) antibody

Anti-SPHK1 antibody IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bt, Hu, Mky, Por, Prim P
50 µg / €379.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) antibody

Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Can, Hu, Rb
50 µg / €379.00
  OriGene Technologies, Inc.

Sphingosine kinase 1 (SPHK1) Control Peptide

Sphingosine kinase 1 (SPHK1) Purified
50 µg / €210.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) control peptide

Sphingosine kinase 1 (SPHK1) Purified
50 µg / €210.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) (Long Form) antibody

Western blot of rat kidney lysate (10 µg/lane), detection of endogenous Sphingosine kinase 1, long form using AP05254PU-N (1 µg/ml), HRP anti-rabbit was used at 1:75k, and developed with Pierce's Super Signal West Femto. Rabbit Aff - Purified Rt WB
10 Blots / €480.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) Control Peptide

Sphingosine kinase 1 (SPHK1) Purified
50 µg / €210.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) pSer225 antibody

Western blot using anti-Mouse SphK1 (pSer225) (Cat.-No AP05255PU-N) on Mouse B-cell lysate. Antibody used at 1 µg/ml with phosphorylated blocking peptide (Lane A) and without (Lane B). Rabbit IgG Aff - Purified Ms WB
10 Blots / €530.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) Control Peptide

Sphingosine kinase 1 (SPHK1) Purified
50 µg / €210.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) antibody

Sphingosine kinase 1 (SPHK1) Rabbit IgG Aff - Purified Ms WB
50 µg / €480.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) (Long Form) antibody

Western blot of rat kidney lysate (10 µg/lane), detection of endogenous Sphingosine kinase 1, long form using AP05277PU-N (3 µg/ml), HRP anti rabbit was used at 1:75k, and developed with Pierce's Super Signal West Femto. Rabbit IgG Purified Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Sphingosine kinase 1 (SPHK1) (N-term) antibody

The anti-SphK1 Pab (AP13921PU-N) is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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