SPI1 / PU.1 antibody

Principal name

SPI1 / PU.1 antibody

Alternative names for SPI1 / PU.1 antibody

Transcription factor PU.1, SPI-1 proto-oncogene, SFPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1, 31 kDa transforming protein

SwissProt ID

P17433 (Mouse), P17947 (Human), P28748 (Schpo), Q6BDS1 (Rat), Q6PKU1 (Pig)

Gene ID

6688 (SPI1), 20375 (Sfpi1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F)

Background of SPI1 / PU.1 antibody

The Ets transcription factor family (Ets-1, Ets-2, Erg-1?3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T lineage commitment and then is dramatically absent following the beta selection checkpoint.

General readings

Rimmele, P., et al. Cancer Res. 70(17): 6757-6766 (2010)
Bonadies, N., et al. Oncogene 29(7): 1062-1072 (2010)
Bonadies, N., et al. Blood 115(2): 331-334 (2010)
Desai, S., et al. J. Immunol. 183(9): 5778-5787 (2009)
Burda, P., et al. Mol. Cancer Res. 7(10): 1693-1703 (2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPI1 / PU.1 antibody

TA303347 (0.03µg/ml) staining of DAUDI lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SPI1 / PU.1 (C-term) antibody

SPI1 Antibody (C-term) (Cat. #TA324571) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SPI1 antibody detected the SPI1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SPI1 / PU.1 antibody

Western blot analysis of extracts from COS-7 cells, using SPI1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPI1 / PU.1 antibody

Immunohistochemistry staining of Paraffin Tonsil tissue by PU.1 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear) Rabbit IgG EPR3158Y Purified Hu P
1 ml / €530.00
  OriGene Technologies, Inc.

SPI1 / PU.1 antibody

WB Suggested Anti-SPI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPI1 / PU.1 antibody

Host: Rabbit; Target Name: Sfpi1; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SPI1 / PU.1 antibody

WB was performed using and the ab against mouse SPI1 at 1:1,000 in TBS-Tween containing 5% milk. Image shows the results for mouse fibroblasts (3T3, lane 1), macrophages (RAW, lane 2) and pre-B cells (HAFTL, lane 3). The position of the protein of interest (expected size: 31 kDa) is indicated on the left. Fibroblasts do not express SPI1, whereas macrophages show a high and pre-B cells a low expression of SPI1. WB performed by Maribel Parra, Center for Genomic Regulation, Barcelona , Spain . Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

SPI1 / PU.1 antibody

Western blot (WB) analysis of PU.1 antibody (Cat.-No.: AP01231PU-N) in extracts from COS-7 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SPI1 / PU.1 (27-37) antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Can, Eq, Hu, Mky, Op, Platypus, Por, Prim, Rb, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

SPI1 / PU.1 (1-122) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Por, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

SPI1 / PU.1 antibody

Western Blot analysis of SPI1 expression in transfected 293T cell line (H00006688-T02) by SPI1 MaxPab polyclonal antibody.

Lane 1: SPI1 transfected lysate(30.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SPI1 / PU.1 antibody

Western Blot analysis of SPI1 expression in transfected 293T cell line by SPI1 monoclonal antibody (M01), clone 1A3.

Lane 1: SPI1 transfected lysate(31.083 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SPI1 / PU.1 antibody

Immunofluorescence of monoclonal antibody to SPI1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2G1 Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SPI1 / PU.1 antibody

SPI1 / PU.1 Rabbit Ms E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SPI1 / PU.1 antibody

Western blot analysis of extracts from COS7 cells, using SPI1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

SPI1 / PU.1 antibody

Western blot analysis of extracts from COS7 cells, using SPI1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

SPI1 / PU.1 antibody

Human Thymus; WB Suggested Anti-SPI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Thymus; SPI1 antibody - middle region (ARP31421_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SPI1 / PU.1 antibody

Human Lymphnode; WB Suggested Anti-SFPI1 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Human Lymphnode; SFPI1 antibody - N-terminal region (ARP37890_T100) in Human Lymphnode cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

SPI1 / PU.1 antibody

Human Muscle; WB Suggested Anti-SPI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; SPI1 antibody - middle region (ARP38241_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SPI1 / PU.1 antibody

Western blot analysis of PU.1 expression in HepG2 (A); TF1 (B) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt, Ze ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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