Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of Spindlin 1 / SPIN1 antibody
Spindlin 1 (Spindlin-like protein 2; SPIN-2 ) protein May play a role in cell-cycle regulation during the transition from gamete to embryo. Subcellular location in the Nucleus. Belongs to the SPIN/STSY family.
Zhang,P.et al. (2008) J. Cell. Physiol. 217 (2), 400-408Yuan,H., et al. (2008) Gene 410 (1), 67-74
Western Blot: SPIN1 Lysate [NBL1-16401] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for SPIN1
Human Brain; WB Suggested Anti-SPIN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; SPIN1 antibody - middle region (ARP52248_P050) in Human Brain cells using Western Blot
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Spindlin 1 Polyclonal Antibody, Unconjugated (bs-0724R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Immunohistochemical analysis of SPIN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SPIN1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.