Spindly / CCDC99 antibody

Principal name

Spindly / CCDC99 antibody

Alternative names for Spindly / CCDC99 antibody

MU-RMS-40.4A, Arsenite-related gene 1 protein, FLJ20364, Coiled-coil domain-containing protein 99, Rrhabdomyosarcoma antigen protein MU-RMS-40.4A

SwissProt ID

Q08DR9 (Bovin), Q3KR99 (Rat), Q923A2 (Mouse), Q96EA4 (Human)

Gene ID

54908 (CCDC99)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Spindly / CCDC99 antibody

Required for the localization of dynein and dyctin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Spindly / CCDC99 antibody

Predicted band size: kDa . Positive control: Hela cell, mouse testis tissue and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: Mouse testis tissue lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Spindly / CCDC99 antibody

Predicted band size: 70 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Spindly / CCDC99 antibody

Host: Rabbit; Target Name: CCDC99; Sample Tissue: Hela Whole cell lysates; Antibody Dilution: 1.0ug/mlSPDL1 is supported by BioGPS gene expression data to be expressed in HeLa Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Spindly / CCDC99 antibody

Western blot analysis on NIH-3T3 cell lysate using CCDC99 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Spindly / CCDC99 antibody

Spindly / CCDC99 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Spindly / CCDC99 antibody

Spindly / CCDC99 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Spindly / CCDC99 antibody

Spindly / CCDC99 Mouse IgG2b 2F4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Spindly / CCDC99 antibody

WB and IP: SPINDLY/CCDC99 Antibody [NB100-61594] - Detection of Human Spindly/CCDC99 on HeLa whole cell lysate using NB100-61594. Spindly/CCDC99 was also immunoprecipitated by rabbit anti-Spindly/CCDC99 antibody NB100-61595. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Spindly / CCDC99 antibody

Western Blot: SPINDLY/CCDC99 Antibody [NB100-61595] - Detection of Human Spindly/CCDC99 by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-Spindly/CCDC99 antibody NB100-61595 used for IP at  3 mcg/mg lysate. Spindly/CCDC99 was also immunoprecipitated  by rabbit anti-Spindly/CCDC99 antibody NB100-61594, which  recognizes an upstream epitope. Detection: Chemiluminescence with  an exposure time of 10 seconds. Rabbit IgG Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Spindly / CCDC99

Spindly / CCDC99 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Spindly / CCDC99

Spindly / CCDC99 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Spindly / CCDC99

Spindly / CCDC99 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SPDL1 overexpression lysate

SPDL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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