Sprouty homolog 4 / SPRY4 antibody

Principal name

Sprouty homolog 4 / SPRY4 antibody

Alternative names for Sprouty homolog 4 / SPRY4 antibody

Spry-4, Sprouty 4

SwissProt ID

A2VDU1 (Bovin), Q9C004 (Human), Q9WTP2 (Mouse)

Gene ID

81848 (SPRY4)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C)

Background of Sprouty homolog 4 / SPRY4 antibody

Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.

General readings

Sasaki A et al. Identification of a dominant negative mutant of Sprouty that potentiates fibroblast growth factor- but not epidermal growth factor-induced ERK activation. J Biol Chem 276:36804-8 (2001). PubMed PMID: 11495895

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Sprouty homolog 4 / SPRY4 (306-322) antibody

Western Blot of Rabbit anti-Sprouty-4 antibody. Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 µg per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: a doublet band ~35 kDa corresponding to human Sprouty-4. Other band(s): none. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Sprouty homolog 4 / SPRY4 antibody

Western blot analysis of Phospho-SPRY4-Y75.ctrl (Cat. #TA324934) in K562 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Sprouty homolog 4 / SPRY4 antibody

WB Suggested Anti-SPRY4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Sprouty homolog 4 / SPRY4 (306-322) antibody

Figure 1. Staining SPRY4 in Heart by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Sprouty homolog 4 / SPRY4 pTyr75 antibody

Dot blot analysis of anti-Phospho-SPRY4 pTyr75 Antibody (Cat.#AP12995PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

Sprouty homolog 4 / SPRY4 (C-term) antibody

Western blot analysis of anti-SPRY4 Antibody (C-term) in Sk-Br-3 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Sprouty homolog 4 / SPRY4 antibody

Sprouty homolog 4 / SPRY4 Rabbit Purified Hu C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Sprouty homolog 4 / SPRY4 antibody

Immunohistochemistry of SPRY4 polyclonal antibody ( Cat # PAB11269 ) shows strong cytoplasmic and membranous staining of tumor cells in human liver tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden. Rabbit Bov, Chimp, Hu, Ms, Rt E, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

Sprouty homolog 4 / SPRY4 (Center) antibody

Human HeLa; WB Suggested Anti-SPRY4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; SPRY4 antibody - middle region (ARP50697_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Sprouty homolog 4 / SPRY4 antibody

Immunohistochemistry-Paraffin: SPRY4 Antibody [NB100-2032] - Immunohistochemistry. anti-Sprouty-4 antibody shows strong cytoplasmic and membranous staining of tumor cells in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Sprouty homolog 4 / SPRY4 (1-299, His-tag)

Sprouty homolog 4 / SPRY4 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Sprouty homolog 4 / SPRY4 (1-299, His-tag)

Sprouty homolog 4 / SPRY4 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Sprouty homolog 4 / SPRY4

Sprouty homolog 4 / SPRY4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Sprouty homolog 4 / SPRY4

Sprouty homolog 4 / SPRY4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

14 Item(s)

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