SRC1 antibody

Principal name

SRC1 antibody

Alternative names for SRC1 antibody

c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

SwissProt ID

P00523 (Chick), P05480 (Mouse), P12931 (Human), Q9WUD9 (Rat)

Gene ID

6714 (SRC), 20779 (Src)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat), Chk (Chicken), Frog, Bov (Bovine), Bt (Bat), Eq (Equine), Fi (Fish), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Tr (Turkey), L (Lizard), Bear, Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of SRC1 antibody

The SRC gene is similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. Mutations in this gene could be involved in the malignant progression of colon cancer. The protein product is pp60vsrc, a cytoplasmic protein with tyrosine-specific protein kinase activity that associates with the cytoplasmic face of the plasma membrane. The protein consists of three domains, an N-terminal SH3 domain, a central SH2 domain and a tyrosine kinase domain. The SH2 and SH3 domains cooperate in the auto-inhibition of the kinase domain. c-Src is phosphorylated on an inhibitory tyrosine near the c-terminus of the protein. This produces a binding site for the SH2 domain which, when bound, facilitates binding of the SH3 domain to a low affinity polyproline site within the linker between the SH2 domain and the kinase domain. Binding of the SH3 domain results in misalignment of residues within the kinase domain's active site inactivating the enzyme. This allows for multiple mechanism for c-Src activation: dephosphorylation of the C-terminal tyrosine by a protein tyrosine phosphatase, binding of the SH2 domain by a competitive phospho-tyrosine residue, as seen in the case of c-Src binding to focal adhesion kinase, or competitive binding of a polyproline binding site to the SH3 domain, as seen in the case of the HIV NEF protein.

General readings

1. Villacis RA  et al. Gene expression profiling in leiomyosarcomas and undifferentiated pleomorphic sarcomas: SRC as a new diagnostic marker. PLoS One 9:e102281 (2014).IHC-P ; Human .PubMed: 25028927
2. Sun Z  et al. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd. J Exp Med 209:1363-77 (2012).PubMed: 22689825

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SRC1 antibody

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SRC Mouse mAb with DAB staining. Mouse IgG1 1F11 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

SRC1 antibody

  Rabbit polyclonal Src (Ab-529) antibody  
Western blot analysis of extracts from HeLa cells, using Src (Ab-529) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Rabbit polyclonal Src (Tyr529) antibody(Phospho-specific)  
Western blot analysis of extracts from COS7 cells, using Src (phospho-Tyr529) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Anti-SRC Rabbit Polyclonal Antibody  
Predicted band size: 60 kDa . Positive control: PC3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Anti-SRC Rabbit Polyclonal Antibody  
Predicted band size: 60 kDa . Positive control: PC3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Anti-SRC (Phospho-Tyr529) Rabbit Polyclonal Antibody  
Predicted band size: 60 kDa . Positive control: HT29 cells untreated or treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HT29 cells untreated with PMA lysate Lane2: HT29 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Rabbit Polyclonal Src Antibody  
Western blot analysis of Src expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Rabbit Polyclonal Src Antibody  
Western blot analysis of Src expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Rabbit Polyclonal Src Antibody  
Western blot analysis of Src expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Rabbit Polyclonal Src (Ser75) Antibody (Phospho-specific)  
Western blot analysis of Src phosphorylation expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Rabbit Polyclonal Src (Tyr418) Antibody (Phospho-specific)  
Western blot analysis of Src phosphorylation expression in serum treated COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 antibody

  Rabbit Polyclonal Src (Tyr529) Antibody (Phospho-specific)  
Western blot analysis of Src phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SRC1 pTyr416 antibody

SRC1 Mouse IgG3 9A6 Biotin Hu, Ms E, F, ICC/IF, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

SRC1 pTyr416 antibody

Phosphospecificity: Whole cell extracts of control (co) and pervanadate stimulated HEK-293 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab src 9A6 (0.5 μg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: co; lane2: pervanadate Mouse IgG3 9A6 Purified Hu, Ms E, F, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SRC1 (N-term) (incl. pos. control) antibody

Detection of endogenous Src: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. 
The immunoblot was probed with AM00146PU-N Src antibody Clone 11F1 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431
Lane 2: SW480
Lane 3: SW620
Lane 4: HT29
Lane 5: MCF-7
Lane 6: MDA-MB231
Lane 7: T47D Mouse IgG1 11F1 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SRC1 antibody

Western blot analysis using SRC antibody Cat.-No AM06226SU-N against truncated SRC-His recombinant protein (Lane 1) and PMA treated THP-1 cell lysate (Lane 2). Mouse IgG2b 4F1E8 Ascites Hu E, WB
0.1 ml / €460.00
  Acris Antibodies GmbH

SRC1 antibody

Western blot analysis using SRC mouse mAb against truncated SRC-His recombinant protein (1) and PMA treated THP-1 cell lysate (2). Mouse IgG2a 5D10C4 Ascites Hu WB
0.1 ml / €440.00
  Acris Antibodies GmbH

SRC1 antibody

Figure 1. Western blot analysis using anti-Src Mab (Cat. #AM11074PU-N) to detect Src in Jurkat cell lysate. Mouse IgG1 17AT28 Purified Hu, Ms IP, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SRC1 (full length) antibody

AM20333PU-N SRC antibody staining of Formalin-Fixed,  Paraffin-Embedded Human Adrenal at 20 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse 5A18 Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SRC1 pTyr418 antibody

Immunohistochemistry (IHC) analyzes of Src pTyr418 antibody (Cat.-No.: AP01687PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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