SREBF1 / SREBP1 antibody

Principal name

SREBF1 / SREBP1 antibody

Alternative names for SREBF1 / SREBP1 antibody

SREBP-1, BHLHD1, Sterol regulatory element-binding transcription factor 1, Sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1

SwissProt ID

O97676 (Pig), P36956 (Human), P56720 (Rat), Q9WTN3 (Mouse)

Gene ID

6720 (SREBF1), 20787 (Srebf1)

Available reactivities

Bov (Bovine), Gt (Goat), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep), Hst (Hamster), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of SREBF1 / SREBP1 antibody

Sterol regulatory element binding-protein 1 (SREBP1/SREBF1) belongs to the SREBP family of transcription factors which are members of the basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factor family (1). SREBP family of transcription factors play a vital role in regulating genes involved in biosynthesis and uptake. More specifically, they modulate the expression of enzymes implicated in the synthesis of cholesterol, fatty acids, triacylglycerol and phospholipids (2). These proteins are synthesized in their inactive form in the ER where they form complex with SREBP cleavage activating protein (SCAP). SCAP restricts SREBPs to the ER under high cholesterol concentrations. It is only under low concentrations that SREBP is transported to the golgi apparatus for activation (1). Here, SREBPs undergo two proteolytic cleavage steps to release the active transcription factor known as nuclear SREBP (nSREBP). Once activated, SREBPs can translocate to the nucleus and bind to sterol response elements (SRE) in the promoter/enhancer regions of target genes (1).
Similarly, the activated SBREBF1 binds to the sterol regulatory element-1 (SRE1) (3). This protein plays a key role in regulating genes for hepatic lipid synthesis (4). It has been reported that cells that are under stressful conditions, such as serum starvation, have an elevated level of SREBF1. Therefore, this protein is also implicated in insulin resistance, diabetes and other diet-related diseases (5). Elevated levels of SREBF1 were also seen in senescent cells and in liver tissues of aging rats (6). Recently it was reported that cells over-expressing SREBF1 are involved in the regulation of myogenesis. SREBF1 is located on human chromosome 17p11.2. It is expressed in a wide variety of tissues, but abundantly expressed in only liver, adrenal gland, and fetal lung tissues. SREBF1 is approximately a 137.6kDa protein (1147 amino acids).

General readings

Wang X et al. Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis. EMBO J 15:1012-20 (1996). PubMed PMID: 8605870
Wang X et al. Purification of an interleukin-1 beta converting enzyme-related cysteine protease that cleaves sterol regulatory element-binding proteins between the leucine zipper and transmembrane domains. J Biol Chem 270:18044-50 (1995). PubMed PMID: 7629113
Sato R et al. Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory element-binding protein-1 (SREBP-1). J Biol Chem 269:17267-73 (1994). PubMed PMID: 8006035

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SREBF1 / SREBP1 antibody

Western Blot: SREBP1 Antibody (2A4) - Western blot analysis of SREBP1 expression in HeLa lysate using anti-SREBP1. Mouse IgG1 2A4 Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SREBF1 / SREBP1 antibody

Western Blot: SREBP1 Antibody - Detection of SREBP1 in human liver (40ug) using anti-SREBP1. Rabbit Purified Bov, Hst, Hu, Ms, Por, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SREBF1 / SREBP1 antibody

Western blot analysis of SREBP-1 expression in TNF treated JK whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SREBF1 / SREBP1 antibody

WB Suggested Anti-SREBF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SREBF1 / SREBP1 antibody

WB Suggested Anti-SREBF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: SP2/0 cell lysate Rabbit IgG Purified Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SREBF1 / SREBP1 antibody

WB Suggested Antibody Titration: 1: 1, 000; Positive Control: HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SREBF1 / SREBP1 antibody

Immunohistochemistry of SREBF1 in human spleen tissue with SREBF1 antibody at 5 ug/mL. Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SREBF1 / SREBP1 (48-403) antibody

Western blot analysis of SREBP-2 expression in HEp-2 (1), MCF-7 (2), LNCap (3), HT1080 (4) and ZR-75-1 (5) using AM26563AF-N Mouse IgG1 1D2 Azide Free Hu WB
0.1 mg / €310.00
  Acris Antibodies GmbH

SREBF1 / SREBP1 (Center) antibody

Western blot analysis of SREBF1 Antibody (Center) (AP18209PU-N) in MDA-MB435 cell line lysates (35ug/lane). SREBF1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SREBF1 / SREBP1 (C-term) antibody

SREBF1 / SREBP1 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SREBF1 / SREBP1 pSer439 antibody

Western blot analysis of extracts from Jurkat cells treated with TNF using Phospho-SREBP-1 (Ser439) antibody AP55903PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

SREBF1 / SREBP1 pSer439 antibody

Western blot analysis of extracts from Jurkat cells treated with TNF using Phospho-SREBP-1 (Ser439) antibody AP55903PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

SREBF1 / SREBP1 antibody

SREBF1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of SREBF1 expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 4B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SREBF1 / SREBP1 antibody

SREBF1 monoclonal antibody (M02), clone 4C11 Western Blot analysis of SREBF1 expression in COLO 320 HSR ( Cat # L020V1 ). Mouse IgG2a 4C11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SREBF1 / SREBP1 antibody

SREBF1 monoclonal antibody (M03), clone 4G4 Western Blot analysis of SREBF1 expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 4G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SREBF1 / SREBP1 antibody

Western blot analysis of SREBF1 in human liver ( Lane 1 ) and mouse liver ( Lane 2 ) with SREBF1 polyclonal antibody ( Cat# PAB12020 ). Rabbit Bov, Gt, Hu, Ms, Rt, Sh WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

SREBF1 / SREBP1 antibody

Western blot analysis of SREBF1 in human liver ( 40 µg ) with SREBF1 polyclonal antibody ( Cat# PAB12021 ). Rabbit Bov, Hst, Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

SREBF1 / SREBP1 pSer439 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using SREBP-1 (Phospho-Ser439) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

SREBF1 / SREBP1 pSer439 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using SREBP-1 (Phospho-Ser439) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

SREBF1 / SREBP1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SREBP-1 (Ab-439) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

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