SREBF2 / SREBP2 antibody

Principal name

SREBF2 / SREBP2 antibody

Alternative names for SREBF2 / SREBP2 antibody

SREBP-2, BHLHD2, Sterol regulatory element-binding transcription factor 2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2

SwissProt ID

A3KNA7 (Danre), Q12772 (Human), Q3T1I5 (Rat), Q3U1N2 (Mouse), Q6GQ26 (Xenla)

Gene ID

6721 (SREBF2), 20788 (Srebf2), 300095

Available reactivities

Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SREBF2 / SREBP2 antibody

Sterol regulatory element binding-protein 1 (SREBP1/SREBF1) belongs to the SREBP family of transcription factors which are members of the basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factor family (1). SREBP family of transcription factors play a vital role in regulating genes involved in biosynthesis and uptake. More specifically, they modulate the expression of enzymes implicated in the synthesis of cholesterol, fatty acids, triacylglycerol and phospholipids (2). These proteins are synthesized in their inactive form in the ER where they form complex with SREBP cleavage activating protein (SCAP). SCAP restricts SREBPs to the ER under high cholesterol concentrations. It is only under low concentrations that SREBP is transported to the golgi apparatus for activation (1). Here, SREBPs undergo two proteolytic cleavage steps to release the active transcription factor known as nuclear SREBP (nSREBP). Once activated, SREBPs can translocate to the nucleus and bind to sterol response elements (SRE) in the promoter/enhancer regions of target genes (1).
Similarly, the activated SREBF2 binds to the sterol regulatory element-1 (SRE1) (3). This is a key protein that regulates genes involved in cholesterol biosynthesis and uptake and it is required for lipid homeostasis (4). SREBF2 is also known to regulate LDL receptor gene (5). Animal studies have reported that mice livers that contained high levels of SREBF2 usually caused an increase in the rate of cholesterol and fatty acid synthesis in that animals when compared to mice whose livers contained normal levels of SREBF2 (6). SREBF2 is universally expressed in fetal and adult tissues. It is approximately a 136.9kDa protein (1141 amino acids).

General readings

Gerin, I., et al. J. Biol. Chem. 285(44): 33652-33661 (2010)
Bailey, S.D., et al. Diabetes Care 33(10): 2250-2253 (2010)
Marquart, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27): 12228-12232 (2010)
Najafi-Shoushtari, S.H., et al. Science 328(5985): 1566-1569 (2010)
Pramfalk, C., et al. J. Lipid Res. 51(6): 1354-1362 (2010)

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SREBF2 / SREBP2 antibody

  Anti-SREBF2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human brain and thyroid cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SREBF2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

  Anti-SREBF2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human brain and breast cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SREBF2 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 (Center) antibody

  Rabbit polyclonal SREBF2 Antibody (Center)  
SREBF2 Antibody (Center) (Cat. #TA324363) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SREBF2 antibody detected the SREBF2 protein (arrow). Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

  Rabbit Polyclonal Anti-SREBF2 Antibody  
WB Suggested Anti-SREBF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:7812500; Positive Control: Transfected 293TSREBF2 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

  Rabbit Polyclonal Anti-SREBF2 Antibody  
WB Suggested Anti-SREBF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Raji cell lysate SREBF2 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells Rabbit IgG Purified Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

  Rabbit Polyclonal Anti-SREBF2 Antibody  
Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

  SREBF2 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SREBF2 (RC208942, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SREBF2. Mouse IgG1 OTI4G1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

SREBF2 - human gene knockout kit via CRISPR

SREBF2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SREBF2 - human gene knockout kit via CRISPR

SREBF2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SREBF2 - human gene knockout kit via CRISPR

SREBF2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SREBF2 - human gene knockout kit via CRISPR

SREBF2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SREBF2 (mGFP-tagged) - Human sterol regulatory element binding transcription factor 2 (SREBF2), 200 uL, >10^7 TU/mL

Lenti ORF particles, SREBF2 (mGFP-tagged) - Human sterol regulatory element binding transcription factor 2 (SREBF2), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, SREBF2 (Myc-DDK tagged) - Human sterol regulatory element binding transcription factor 2 (SREBF2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, SREBF2 (Myc-DDK tagged) - Human sterol regulatory element binding transcription factor 2 (SREBF2) , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Srebf2 (GFP-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Srebf2 (GFP-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2), 200 uL, >10^7 TU/mL  
200 µl / €1,950.00
  OriGene Technologies, Inc.

Lenti ORF particles, Srebf2 (Myc-DDK-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Srebf2 (Myc-DDK-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2), 200 uL, >10^7 TU/mL  
200 µl / €1,760.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Srebf2 (GFP-tagged ORF) - Rat sterol regulatory element binding transcription factor 2 (Srebf2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Srebf2 (GFP-tagged ORF) - Rat sterol regulatory element binding transcription factor 2 (Srebf2), 200 uL, >10^7 TU/mL  
200 µl / €2,330.00
  OriGene Technologies, Inc.

Lenti ORF particles, Srebf2 (Myc-DDK-tagged ORF) - Rat sterol regulatory element binding transcription factor 2 (Srebf2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Srebf2 (Myc-DDK-tagged ORF) - Rat sterol regulatory element binding transcription factor 2 (Srebf2), 200 uL, >10^7 TU/mL  
200 µl / €2,101.00
  OriGene Technologies, Inc.

17 Item(s)

per page
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