SREBF2 / SREBP2 antibody

Principal name

SREBF2 / SREBP2 antibody

Alternative names for SREBF2 / SREBP2 antibody

SREBP-2, BHLHD2, Sterol regulatory element-binding transcription factor 2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2

SwissProt ID

A3KNA7 (Danre), Q12772 (Human), Q3T1I5 (Rat), Q3U1N2 (Mouse), Q6GQ26 (Xenla)

Gene ID

6721 (SREBF2), 67211 (Armc10), 20788 (Srebf2)

Available reactivities

Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Por (Porcine), African clawed frog, Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SREBF2 / SREBP2 antibody

Sterol regulatory element binding-protein 1 (SREBP1/SREBF1) belongs to the SREBP family of transcription factors which are members of the basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factor family (1). SREBP family of transcription factors play a vital role in regulating genes involved in biosynthesis and uptake. More specifically, they modulate the expression of enzymes implicated in the synthesis of cholesterol, fatty acids, triacylglycerol and phospholipids (2). These proteins are synthesized in their inactive form in the ER where they form complex with SREBP cleavage activating protein (SCAP). SCAP restricts SREBPs to the ER under high cholesterol concentrations. It is only under low concentrations that SREBP is transported to the golgi apparatus for activation (1). Here, SREBPs undergo two proteolytic cleavage steps to release the active transcription factor known as nuclear SREBP (nSREBP). Once activated, SREBPs can translocate to the nucleus and bind to sterol response elements (SRE) in the promoter/enhancer regions of target genes (1).
Similarly, the activated SREBF2 binds to the sterol regulatory element-1 (SRE1) (3). This is a key protein that regulates genes involved in cholesterol biosynthesis and uptake and it is required for lipid homeostasis (4). SREBF2 is also known to regulate LDL receptor gene (5). Animal studies have reported that mice livers that contained high levels of SREBF2 usually caused an increase in the rate of cholesterol and fatty acid synthesis in that animals when compared to mice whose livers contained normal levels of SREBF2 (6). SREBF2 is universally expressed in fetal and adult tissues. It is approximately a 136.9kDa protein (1141 amino acids).

General readings

Gerin, I., et al. J. Biol. Chem. 285(44): 33652-33661 (2010)
Bailey, S.D., et al. Diabetes Care 33(10): 2250-2253 (2010)
Marquart, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27): 12228-12232 (2010)
Najafi-Shoushtari, S.H., et al. Science 328(5985): 1566-1569 (2010)
Pramfalk, C., et al. J. Lipid Res. 51(6): 1354-1362 (2010)

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SREBF2 / SREBP2 antibody

Predicted cell location: Cytoplasm . Positive control: Human brain and thyroid cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SREBF2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

Predicted cell location: Cytoplasm . Positive control: Human brain and breast cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SREBF2 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 (Center) antibody

SREBF2 Antibody (Center) (Cat. #TA324363) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SREBF2 antibody detected the SREBF2 protein (arrow). Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

WB Suggested Anti-SREBF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:7812500; Positive Control: Transfected 293TSREBF2 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

WB Suggested Anti-SREBF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Raji cell lysate SREBF2 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells Rabbit IgG Purified Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SREBF2 / SREBP2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SREBF2 (RC208942, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SREBF2. Mouse IgG1 OTI4G1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SREBF2 / SREBP2 (580-640) antibody

SREBF2 / SREBP2 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SREBF2 / SREBP2 antibody

SREBF2 polyclonal antibody (A01), Lot # 051213JC01 Western Blot analysis of SREBF2 expression in IMR-32 ( Cat # L008V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SREBF2 / SREBP2 antibody

mouse glomerular endothelial; WB Suggested Anti-SREBF2 Antibody . . Positive Control: Lane 1: 50ug mouse glomerular endothelial lysate . Primary Antibody Dilution : 1:1000 . Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000 . Submitted by: Xiaoxin Wang, UC Denver; SREBF2 antibody - middle region (AVARP09037_P050) in mouse glomerular endothelial cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

SREBF2 / SREBP2 (Center) antibody

Human 293T; WB Suggested Anti-SREBF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:7812500. Positive Control: Transfected 293T. SREBF2 is supported by BioGPS gene expression data to be expressed in HEK293T.; SREBF2 antibody - middle region (P100696_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms, Por, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SREBF2 / SREBP2 antibody

SREBF2 / SREBP2 Rabbit IgG Aff - Purified Ms, Rt WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SREBF2 / SREBP2 antibody

Western  Blot:  SREBP2 Antibody [NBP1-33547] – Western blot analysis of SREBP2 on Molt-4 lysates using NBP1-33547 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel. Rabbit IgG Hu ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

SREBF2 / SREBP2 antibody

SREBF2 / SREBP2 Mouse IgG1 1D2 Purified Hu WB
50 µg / €320.00
  Novus Biologicals Inc.

SREBF2 / SREBP2 antibody

Western Blot: SREBP2 Antibody [NBP1-71880] - Detection of Human SREBP2 by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-SREBP2 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate.  SREBP2 was also immunoprecipitated by rabbit anti-SREBP2 antibody which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SREBF2 / SREBP2

SREBF2 / SREBP2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SREBF2 / SREBP2

SREBF2 / SREBP2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

ELISA Kits

Catalog No.    

SREBF2 Cell-Based Translocation Assay Kit

SREBF2 Cell-Based Translocation Assay Kit  
1 kit / €420.00
  Abnova Taiwan Corp.

SREBF2 Transcription Factor Assay Kit

SREBF2 Transcription Factor Assay Kit  
1 kit / €510.00
  Abnova Taiwan Corp.

19 Item(s)

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