SS-A / Ro52 / TRIM21 antibody

Principal name

SS-A / Ro52 / TRIM21 antibody

Alternative names for SS-A / Ro52 / TRIM21 antibody

SSA1, TRIM21, RNF81, Ro(SS-A), Sjoegren syndrome type A antigen, E3 ubiquitin-protein ligase TRIM21, Tripartite motif-containing protein 21, RING finger protein 81

SwissProt ID

P19474 (Human), Q62191 (Mouse), Q7YRV4 (Bovin)

Gene ID

6737 (TRIM21), 359715

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Por (Porcine), Rt (Rat), Mky (Monkey), Prim (Primate), Can (Canine), GP (Guinea Pig), Rb (Rabbit), Eq (Equine), Ze (Zebrafish)

Available hosts

Human, Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunodiffusion (ID), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of SS-A / Ro52 / TRIM21 antibody

TRIM21 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM21 is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus.This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Chan EK, Di Donato F, Hamel JC, Tseng CE, Buyon JP. 52-kD SS-A/Ro: genomic structure and identification of an alternatively spliced transcript encoding a novel leucine zipper-minus autoantigen expressed in fetal and adult heart.
J Exp Med. 1995 Oct 1;182(4):983-92. PMID: 7561701

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SS-A / Ro antibody

SS-A / Ro Human Purified Hu E, ID
2 ml / €320.00
  Acris Antibodies GmbH

SS-A / Ro52 / TRIM21 antibody

  Rabbit Polyclonal antibody to SSA1 (tripartite motif-containing 21)  
Sample (30 ug of whole cell lysate). A: Molt-4 . 10% SDS PAGE. TA308743 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 (C-term) antibody

  Goat Anti-TRIM21 (SSA1) Antibody  
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with TA311258 (1ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311258 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 antibody

  Rabbit anti-TRIM21 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using TRIM21 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 antibody

  Rabbit Polyclonal Anti-TRIM21 Antibody  
WB Suggested Anti-TRIM21 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T Rabbit IgG Purified Can, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 antibody

  Rabbit Polyclonal Anti-TRIM21 Antibody  
WB Suggested Anti-TRIM21 Antibody Titration: 1.25 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 antibody

  Rabbit Polyclonal Anti-TRIM21 Antibody  
WB Suggested Anti-TRIM21 Antibody Titration: 1.25 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Eq, Hu, Por, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 antibody

  Rabbit Polyclonal Anti-TRIM21 Antibody  
Western blot analysis of TRIM21 in human lung tissue lysate with TRIM21 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 antibody

  Rabbit Polyclonal Anti-TRIM21 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using (TRIM21 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 antibody

  Rabbit Polyclonal Anti-TRIM21 rabbit polyclonal antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Lovo cells, A549 cells, hela cells, Primary antibody: (TRIM21 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SS-A / Ro52 / TRIM21 (386-398) antibody

Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Hu, Mky, Prim E, P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

TRIM21 - human gene knockout kit via CRISPR

TRIM21 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TRIM21 - human gene knockout kit via CRISPR

TRIM21 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM21 - human gene knockout kit via CRISPR

TRIM21 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TRIM21 - human gene knockout kit via CRISPR

TRIM21 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SS-A / Ro

SS-A  / Ro Bovine Aff - Purified > 90 % by SDS-PAGE
0.2 mg / €880.00
  Acris Antibodies GmbH

SS-A / Ro

SS-A / Ro Bovine Aff - Purified Immuno-affinity chromatography. Purification is verified by SDS-PAGE. Other antigens are not detected with ELISA assays. Spleen
1 kU / €1,350.00
  Acris Antibodies GmbH

SS-A / Ro52 / TRIM21

  Recombinant protein of human tripartite motif-containing 21 (TRIM21)  
SS-A / Ro52 / TRIM21 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

TRIM21 overexpression lysate

TRIM21 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, TRIM21 (mGFP-tagged) - Human tripartite motif containing 21 (TRIM21), 200 uL, >10^7 TU/mL

Lenti ORF particles, TRIM21 (mGFP-tagged) - Human tripartite motif containing 21 (TRIM21), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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