SSRP1 / FACT80 antibody

Principal name

SSRP1 / FACT80 antibody

Alternative names for SSRP1 / FACT80 antibody

FACT complex subunit SSRP1, FACTp80, T160, Facilitates chromatin transcription complex subunit SSRP1, FACT 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit, Structure-specific recognition protein 1, Recombination signal sequence recognition protein 1

SwissProt ID

Q04678 (Chick), Q04931 (Rat), Q08943 (Mouse), Q08945 (Human), Q9W602 (Xenla)

Gene ID

6749 (SSRP1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), African clawed frog, Dros (Drosophila), Ze (Zebrafish), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of SSRP1 / FACT80 antibody

SSRP1 is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. SSRP1 contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. It also functions as a co-activator of the transcriptional activator p63.The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Li,Y., (2007) J. Biol. Chem. 282 (10), 6936-6945

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SSRP1 / FACT80 antibody

Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA308461 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SSRP1 / FACT80 (308-524) antibody

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-SSRP1 antibody. Proteins were detected using a goat anti-mouse Ig secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2b 10D1 Purified Hu ChIP, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SSRP1 / FACT80 antibody

293E cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SSRP1 Antibody (clone 3E4). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 3E4 Purified Hu ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SSRP1 / FACT80 antibody

WB Suggested Anti-SSRP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SSRP1 / FACT80 antibody

SSRP1 / FACT80 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SSRP1 / FACT80 antibody

WB was performed on nuclear extracts from HeLa cells (20 ug) using the antibody against SSRP1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the right. The location of the protein of interest is indicated by an arrow. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

SSRP1 / FACT80 antibody

WB was performed on nuclear extracts from HeLa cells (20 ug) using the antibody against hSSRP1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated by an arrow. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

SSRP1 / FACT80 (308-524) antibody

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-SSRP1 antibody. Proteins were detected using a goat anti-mouse Ig secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2b 10D1 Purified Hu ChIP, ICC/IF, IP, WB
25 µg / €210.00
  Acris Antibodies GmbH

SSRP1 / FACT80 antibody

SSRP1 / FACT80 Rabbit IgG Purified Hu, Ms IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

SSRP1 / FACT80 antibody

SSRP1 / FACT80 Rabbit IgG Purified Hu, Ms IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

SSRP1 / FACT80 (C-term) antibody

Western blot analysis of SSRP1 / FACT80 Antibody (C-term) Cat.-No AP54054PU-N in CEM cell line lysates (35ug/lane). This demonstrates the SSRP1 antibody detected the SSRP1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SSRP1 / FACT80 antibody

SSRP1 / FACT80 Rabbit Hu, Ms IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SSRP1 / FACT80 antibody

SSRP1 / FACT80 Hu, Ms
0.1 mg / €400.00
  Abnova Taiwan Corp.

SSRP1 / FACT80 antibody

Western blot was performed using nuclear extracts from HeLa cells ( HeLa NE, 20 µg ) and the SSRP1 polyclonal antibody ( Cat # PAB14118 ) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. Rabbit Purified Hu WB
50 µg / €390.00
  Abnova Taiwan Corp.

SSRP1 / FACT80 antibody

Human OVCAR3; WB Suggested Anti-SSRP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: OVCAR-3 cell lysate. SSRP1 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells.; SSRP1 antibody - middle region (ARP31141_P050) in Human OVCAR3 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SSRP1 / FACT80 antibody

Transfected 293T; WB Suggested Anti-SSRP1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; SSRP1 antibody - N-terminal region (ARP38249_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SSRP1 / FACT80 antibody

Human Spleen; WB Suggested Anti-SSRP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; SSRP1 antibody - middle region (P100799_P050) in Human Spleen cells using Western Blot Rabbit Aff - Purified Bov, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SSRP1 / FACT80 antibody

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-SSRP1 antibody. Proteins were detected using a goat anti-mouse Ig secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2b 10D7 Purified Hu ChIP, ICC/IF, IP, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SSRP1 / FACT80 antibody

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-SSRP1 antibody (clone 3E4). Proteins were detected using a goat anti-mouse Ig secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 3E4 Purified Hu ICC/IF, IP, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SSRP1 / FACT80 antibody

SSRP1 / FACT80 Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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