Hu (Human), Xen (Xenopus), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)
Goat, Mouse, Rabbit
Immunocytochemistry/Immunofluorescence (ICC/IF), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of STAG1 / Stromal antigen 1 antibody
This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008].
Western Blot: SA1 Antibody [NB100-298] - ample: Nuclear extract from ~500,000 HeLa cells. Antibody: Affinity purified goat anti-SA1 used at 0.5 mcg/ml. Detection: Chemiluminescence with 15 sec. exposure.
STAG1 antibody [C1C2], Internal detects STAG1 protein at cytoplasm and nucleus on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. STAG1 antibody [C1C2], Internal (GTX115385) diluted at 1:250.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STAG1(GTX115385) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.