STAP2 / BKS antibody

Principal name

STAP2 / BKS antibody

Alternative names for STAP2 / BKS antibody

STAP-2, BRK substrate, Signal-transducing adaptor protein 2, Breast tumor kinase substrate

SwissProt ID

Q8R0L1 (Mouse), Q9UGK3 (Human)

Gene ID

55620 (STAP2), 106766 (Stap2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Goat, Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of STAP2 / BKS antibody

This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).

General readings

1. Ikeda O, Miyasaka Y, Sekine Y, Mizushima A, Muromoto R, Nanbo A, Yoshimura A, Matsuda T. STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation. Biochem Biophys Res Commun. 2009 Jun 19;384(1):71-5. PMID: 19393627
2. Ikeda O, Mizushima A, Sekine Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T. Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells. Cancer Sci. 2011 Apr;102(4):756-61. PMID: 21205088
3. Ikeda O, Sekine Y, Mizushima A, Nakasuji M, Miyasaka Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T. Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells.
J Biol Chem. 2010 Dec 3;285(49):38093-103. PMID: 20929863
4. Sekine Y, Ikeda O, Tsuji S, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T. Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells.
J Immunol. 2009 Dec 15;183(12):7966-74. PMID: 19933863

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

STAP2 / BKS (436-449) antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Hu, Prim E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

STAP2 / BKS (C-term) antibody

AP23706PU-N STAP2 antibody staining of Human Breast lysate at 0.3 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

STAP2 / BKS (C-term) antibody

TA302720 (0.03µg/ml) staining of human heart lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAP2 / BKS antibody

Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. TA308170 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAP2 / BKS (C-term) antibody

TA329096 (1µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAP2 / BKS (Center) antibody

Formalin fixed and paraffin embedded human brain tissue stained with STAP2 Antibody (Center) Cat.-No AP54064PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of STAP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

STAP2 / BKS antibody

STAP2 / BKS Goat Purified Hu WB
0.1 mg / €550.00
  Acris Antibodies GmbH

STAP2 / BKS antibody

STAP2 / BKS Mouse IgG2a 6C7 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

STAP2 / BKS antibody

STAP2 polyclonal antibody ( Cat # PAB6042 ) staining ( 0.03 µg/ml ) of human heart lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

STAP2 / BKS antibody

Western blot analysis of STAP2 expression in HCT116 (A); SP2/0 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

STAP2 / BKS antibody

Western blot analysis of STAP2 expression in HCT116 (A); SP2/0 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

STAP2 / BKS antibody

Western blot analysis of STAP2 expression in HCT116 (A); SP2/0 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

STAP2 / BKS antibody

Immunohistochemistry-Paraffin: STAP2 Antibody [NB300-987] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

STAP2 / BKS antibody

Western  Blot:  STAP2 Antibody [NBP1-33643] – Western blot analysis of STAP2 on HCT116 lysates using NBP1-33643 at a dilution of 1:1000. 30ug whole cell lysate loaded on 10% SDS PAGE gel. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

STAP2 / BKS antibody

STAP2 / BKS Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

STAP2 / BKS antibody

Immunohistochemistry-Paraffin: STAP2 Antibody [NBP1-88585] -  staining of human bone marrow shows strong cytoplasmic positivity in subsets of cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

STAP2 / BKS antibody

Immunohistochemistry-Paraffin: STAP2 Antibody [NBP1-88586] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

STAP2 / BKS (transcript variant 2)

STAP2 / BKS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

STAP2 / BKS

STAP2 / BKS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

STAP2 / BKS

STAP2 / BKS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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