STAT1 antibody

Principal name

STAT1 antibody

Alternative names for STAT1 antibody

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

SwissProt ID

P42224 (Human), P42224-1, P42224-2, P42225 (Mouse), Q764M5 (Pig)

Gene ID

6772 (STAT1), 20846 (Stat1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Rb (Rabbit), Mky (Monkey), Sh (Sheep)

Available hosts

Rabbit, Mouse, Hamster

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), Dot blot (Dot), EPair for Elisa (EPair)

Background of STAT1 antibody

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.
Specific functions of the STAT proteins are poorly defined at this time. They are thought to be involved in a variety of systems including antiviral responses and cell transformation. STAT1 deficient knock-out mice are unable to induce IFN regulated genes and are extremely susceptible to viral diseases. After phosphorylation, STAT1 and STAT2 form heterodimers that function as more potent inducers of transcription than the STAT1 homodimer.
STAT1 is known to associate with the transcription factors p48, Sp1, and p300. STAT1 is activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family. STAT1 alpha and STAT1 beta isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1 beta lacks 38 carboxy terminal amino acids. Contained within these 38 terminal amino acids of STAT1 alpha is a critical serine residue (Ser 727) whose phosphorylation is required for maximal transcription activity induced by IFN gamma.

General readings

Mikhak,Z., et al., (2006) J. Immunol. 176 (8), 4959-4967

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

STAT1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-STAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500542) Mouse IgG1 OTI4H9 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

STAT1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-STAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500565, Dilution 1:50) Mouse IgG1 OTI13B10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

STAT1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-STAT1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500566, Dilution 1:50) Mouse IgG1 OTI15H3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

STAT1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PDGFRB mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

STAT1 antibody

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STAT1 mouse monoclonal antibody. (Dilution 1:50) Mouse 4H9 Aff - Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

STAT1 antibody

STAT1 Antibody (15H3) [NBP1-48013] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-STAT1mouse monoclonal antibody. (Dilution 1:50) Mouse 15H3 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

STAT1 (alpha) (C-term) antibody

Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1a antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Western blot analysis of extracts from 293/3T3/Jurkat, using STAT1 (Ab-727) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Western blot analysis of extracts from COLO205 cells, using STAT1 (Ab-701) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Western blot analysis of extracts from Jurkat cells, COLO205 cells and HUVEC cells, using STAT1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Western blot analysis of STAT1 in human small intestine tissue lysate with STAT1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Immunoblot of human peripheral blood mononuclear cell (lane 1) and mouse splenocyte (lane 2) lysates with 2 ug/ml of Anti-Human STAT1 Purified.  Bands were visualized using Anti-Armenian Hamster IgG HRP. Hamster ATO-2F5 Purified Hu WB
25 µg / €120.00
  OriGene Technologies, Inc.

STAT1 antibody

Predicted band size: 87 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/250-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/250 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Predicted band size: 87 kDa . Positive control: Hela, A431 and Jurkat cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1-3: Hela, A431 and Jurkat cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

STAT1 Antibody (C-term) (Cat. #TA324682) western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).This demonstrates the STAT1 antibody detected the STAT1 protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

STAT1 Antibody(Cat. #TA324835) western blot analysis in CEM,Hela cell line lysates (35ug/lane).This demonstrates the STAT1 antibody detected the STAT1 protein (arrow). Mouse IgG1 1141CT26.2.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Western blot analysis of STAT1 expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against STAT1. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 SAT-84 Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

Western blot detection of STAT1 in Hela,MCF7,COS7,SW480 and A549 cell lysates using STAT1 mouse mAb (1:1000 diluted).Predicted band size:91KDa.Observed band size:91KDa. Mouse IgG2a 6C2-F7-D12 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (RC201075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1. Mouse IgG1 OTI9B12 Purified Can, Hu, Mky WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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