STAT3 antibody

Principal name

STAT3 antibody

Alternative names for STAT3 antibody

STAT-3, Acute-phase response factor, APRF, Signal transducer and activator of transcription 3

SwissProt ID

P40763 (Human), P42227 (Mouse), P52631 (Rat), P61635 (Bovin), Q19S50 (Pig), Q6DV79 (Chick)

Gene ID

6774 (STAT3), 20848 (Stat3), 25125

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Hst (Hamster), Mky (Monkey), Rb (Rabbit), Sh (Sheep), Prim (Primate)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Dot blot (Dot)

Background of STAT3 antibody

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.
Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
STAT1 and STAT2 are induced by IFN alpha and forn 1, a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.

General readings

1. Akira, S., Nishio, Y., Inoue, M., Wang, X.-J., Wei, S., Matsusaka, T., Yoshida, K., Sudo, T., Naruto,
M., Kishimoto, T. Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related
transcription factor involved in the gp130-mediated signaling pathway. Cell 77: 63-71, 1994.
2. Bhattacharya, S., Schindler, C. Regulation of Stat3 nuclear export. J. Clin. Invest. 111: 553-559,
2003.
3. Bong, Y.-S., Lee, H.-S., Carim-Todd, L., Mood, K., Nishanian, T. G., Tessarollo, L., Daar, I.
O. ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3. Proc. Nat. Acad.
Sci. 104: 17305-17310, 2007.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

STAT3 pTyr705 antibody

Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody Cat.-No AP02344PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

STAT3 pTyr705 antibody

Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody Cat.-No AP02344PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

STAT3 antibody

The phospho-STAT3-Ser727 Pab (Cat. #TA302174) is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA). Rabbit Purified Hu, Ms ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

The Phospho-STAT3-S727 Pab (Cat. #3260a) is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA). Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-STAT3 (TA500177) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min). Mouse IgG1 OTI21E7 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

STAT3 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between NFKB1 and STAT3 HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-STAT3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 4D6 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

STAT3 antibody

Human kidney; Immunohistochemistry with Human kidney lysate tissue; STAT3 antibody - N-terminal region (ARP38253_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

STAT3 (C-term) (Isoform 1+2) antibody

TA302716 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

TA303135 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of STAT3 in human small intestine tissue lysate with STAT3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

STAT3 Antibody (pS727) (Cat. #TA324629) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow). Rabbit IgG Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of STAT3 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of STAT3 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of STAT3 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of extracts from HeLa cells, using STAT3 (phospho-Tyr705) antibodyThe lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of extracts from HeLa/3T3 cells, using STAT3 (Ab-705) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STAT3 antibody

Western blot analysis of extracts of Untreated and treted by IFN, using Phospho-STAT3-Y705 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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