STRAD alpha / LYK5 antibody

Principal name

STRAD alpha / LYK5 antibody

Alternative names for STRAD alpha / LYK5 antibody

STRADA, STRAD, STE20-related adapter protein, NY-BR-96, STE20-related kinase adapter protein alpha

SwissProt ID

Q3UUJ4 (Mouse), Q5E9J9 (Bovin), Q5ZK47 (Chick), Q7RTN6 (Human), Q7TNZ6 (Rat)

Gene ID

92335 (STRADA)

Available reactivities

Hu (Human), Mky (Monkey), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of STRAD alpha / LYK5 antibody

STE20-related kinase adapter protein alpha (STRADA) is a catalytically inactive pseudokinase protein located on human chromosome 17q23.3 (1). In its association with calcium binding protein 39 (CAB39), it regulates the activity of Serine/Threonine Kinase 11 (STK11/LKB1) tumor suppressors (2). LKB1 was discovered in 1988 and ever since then extensive research has been conducted in understanding how this protein might be regulated. It was reported that LKB1 mediates its cellular functions through interactions with a number of proteins including CAB39 and STRADalpha (3). CAB39 and STRADalpha are identified as key proteins required for the growth suppressive function of LKB1. Studies reported that association of STRADA with ATP or CAB39 is essential for LKB1 activation and, STRADA is also required for LKB1-induced G1 cell cycle arrest (4).
It has been deduced that STRADA interacts with CAB39 via its C-terminal Trp-Glu-Phe residues (WEF motif) and any mutations in these residues abolishes this interaction. Although such STRADA mutants are unable to interact directly with CAB39, they can still form a heterotrimeric complex with LKB1 and CAB39 (5). This demonstrates that STRADA possesses multiple interaction sites for CAB39 and LKB1 that are separate from the WEF motif. Other studies have identified that deletion of 180 C-terminal residues of STRADA causes severe human developmental and epileptic syndrome termed polyhydraminos, megalencephaly, and symptomatic epilepsy (PMSE) syndrome (6).
STRADA is approximately a 58kDa protein (431 amino acids).

General readings

Avizienyte E., et al. (1998) Cancer Res. 58: 2087-2090.Baas A F., et al. (2003) EMBO J. 22: 3062-3072.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

STRAD alpha / LYK5 antibody

Western blot analysis of extracts from HepG2 cells, using STRAD antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STRAD alpha / LYK5 antibody

WB Suggested Anti-LYK5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:500; Positive Control: Human Spleen Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

STRAD alpha / LYK5 antibody

WB Suggested Anti-LYK5 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate.STRADA is strongly supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

STRAD alpha / LYK5 antibody

Western blot analysis on HepG2 cell lysate using STRAD Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STRAD alpha / LYK5 Control Peptide

STRAD alpha / LYK5
0.25 mg / €250.00
  Acris Antibodies GmbH

STRAD alpha / LYK5 (N-term) antibody

Western Blot of STRADA antibody (AP09873PU-N at 1/500 dilution) Rabbit Aff - Purified Hu, Mky E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

STRAD alpha / LYK5 (N-term) antibody

The anti-LYK5 Pab is used in Western blot to detect LYK5 in mouse brain tissue lysate Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

STRAD alpha / LYK5 (C-term) antibody

The anti-LYK5 Pab is used in Western blot to detect LYK5 in Jurkat tissue lysate Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

STRAD alpha / LYK5 (C-term) antibody

STRAD alpha / LYK5 Rabbit Aff - Purified Hu, Mky E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

STRAD alpha / LYK5 antibody

Western Blot analysis of STRADA expression in transfected 293T cell line (H00092335-T01) by STRADA MaxPab polyclonal antibody.

Lane 1: LYK5 transfected lysate(38.28 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

STRAD alpha / LYK5 antibody

STRAD alpha / LYK5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

STRAD alpha / LYK5 antibody

Immunoprecipitation of STRADA transfected lysate using anti-STRADA MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with STRADA MaxPab mouse polyclonal antibody (B01) (H00092335-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

STRAD alpha / LYK5 antibody

STRAD alpha / LYK5 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

STRAD alpha / LYK5 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between STK11 and STRADA HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-STRADA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 4E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

STRAD alpha / LYK5 antibody

Western blot analysis of LYK5 in A-549 cell lysate in the ( A ) absence and ( B ) presence of immunizing peptide using LYK5 polyclonal antibody ( Cat # PAB0376 ) at 1 µg/ml. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

STRAD alpha / LYK5 antibody

The STRADA polyclonal antibody ( Cat # PAB2696 ) is used in Western blot to detect STRADA in mouse brain tissue lysate. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

STRAD alpha / LYK5 antibody

Western blot analysis of extracts from HepG2 cells, using STRAD Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

STRAD alpha / LYK5 antibody

Western blot analysis of extracts from HepG2 cells, using STRAD Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

STRAD alpha / LYK5 antibody

Human Spleen; WB Suggested Anti-LYK5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:500. Positive Control: Human Spleen; LYK5 antibody - N-terminal region (ARP49076_P050) in Human Spleen cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

STRAD alpha / LYK5 antibody

Human 721_B; Host:Rabbit. Target Name:STRADA. Sample Tissue:721_B. Antibody Dilution: 1.0ug/ml. STRADA is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; STRADA antibody - C-terminal region (ARP49077_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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