STRAD beta / ILPIP antibody
Principal name
STRAD beta / ILPIP antibody
Alternative names for STRAD beta / ILPIP antibody
Pseudokinase ALS2CR2, CALS-21, STRADB, ALS2CR2, PRO1038, STE20-related kinase adapter protein beta
Ncbi ID
9606, NP_001193793.1, NP_061041, NP_061041.2, NP_766244.4, NM_172656, NM_018571
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of STRAD beta / ILPIP antibody
This gene encodes a protein that belongs to the serine/threonine protein kise STE20 subfamily. One of the active site residues in the protein kise domain of this protein is altered, and it is thus a pseudokise. This protein is a component of a complex involved in the activation of serine/threonine kise 11, a master kise that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kise from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kise. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 sigl transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Altertively spliced transcript variants encoding different isoforms have been found for this gene.
ALS2CR2 polyclonal antibody (A01), Lot # 061025JCS1 Western Blot analysis of ALS2CR2 expression in HeLa ( Cat # L013V1 ).
Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
ALS2CR2 polyclonal antibody ( Cat # PAB6344 ) (3.8µg/ml) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
HEK293 overexpressing ALS2CR2 and probed with ALS2CR2 polyclonal antibody ( Cat # PAB6344 ) (mock transfection in first lane), tested by Origene.
ALS2CR2 polyclonal antibody ( Cat # PAB6344 ) staining ( 1ug/ml ) of human heart lysate ( RIPA buffer, 30ug total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of ALS2CR2 (arrow) using ALS2CR2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALS2CR2 gene (Lane 2)
Immunohistochemistry of STRADB in human brain tissue with STRADB polyclonal antibody ( Cat # PAB13114 ) at 10 µg/ml.
Western blot analysis of STRADB in human brain lysate with STRADB polyclonal antibody ( Cat # PAB13114 ) at ( A ) 1 and ( B ) 2 µg/ml.
Immunohistochemistry of STRADB in human heart tissue with STRADB polyclonal antibody ( Cat # PAB13115 ) at 5 µg/ml.
Western blot analysis of STRADB in human heart lysate with STRADB polyclonal antibody ( Cat # PAB13115 ) at 0.5 ( lane A ), 1 ( lane B ), and 2 ( lane C ) µg/ml, respectively.
Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
HEK293 overexpressing ILPIP and probed with the antibody (mock transfection in first lane).
Antibody staining (1µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence
Western blot analysis of ILPIP in human brain lysate with ILPIP antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry of ILPIP in human brain tissue with ILPIP antibody at 10 ug/ml.
Western blot analysis of ILPIP in human heart lysate with ILPIP antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/ml, respectively.
Immunohistochemistry of ILPIP in human heart tissue with ILPIP antibody at 5 ug/ml.
HEK293 overexpressing ILPIP (RC203432) and probed with TA311479 (mock transfection in first Lane).
TA311479 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Predicted band size: kDa. Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 minutes)
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using STRADB antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: kDa. Positive control: Mouse pancreas and spleen tissue, mouse heart tissue and 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1-4: Mouse pancreas and spleen tissue, mouse heart tissue and 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 minutes)
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using STRADB antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306142 | STRAD beta / ILPIP (Center) antibody |
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| Rabbit Polyclonal ILPIP Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€371.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA306143 | STRAD beta / ILPIP (C-term) antibody |
||||||
| Rabbit Polyclonal ILPIP Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, P, WB |
0.1 mg /
€371.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA311479 | STRAD beta / ILPIP (C-term) antibody |
||||||
| Goat Anti-STRADB / ALS2CR2 Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu | P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA324159 | STRAD beta / ILPIP antibody |
||||||
| Anti-STRADB rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA324160 | STRAD beta / ILPIP antibody |
||||||
| Anti-STRADB rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP07502PU-N | STRAD beta / ILPIP antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| AP08962PU-N | STRAD beta / ILPIP 406-418) antibody |
||||||
 |
Goat | Aff - Purified | Hu | E, P, WB |
50 µg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| +2 additional images |
|||||||
| AP13724PU-N | STRAD beta / ILPIP (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203432 | STRADB - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203432BN | STRADB - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203432LP | STRADB - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203432RB | STRADB - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN316908 | Stradb - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN316908BN | Stradb - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN316908LP | Stradb - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN316908RB | Stradb - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC203432L2V | Lenti ORF particles, STRADB (mGFP-tagged) - Human STE20-related kinase adaptor beta (STRADB), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC203432L1V | Lenti ORF particles, STRADB (Myc-DDK tagged) - Human STE20-related kinase adaptor beta (STRADB), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206623L2V | Lenti ORF particles, Stradb (GFP-tagged) - Mouse STE20-related kinase adaptor beta (Stradb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| MR206623L1V | Lenti ORF particles, Stradb (Myc-DDK-tagged) - Mouse STE20-related kinase adaptor beta (Stradb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
