Streptavidin antibody

Principal name

Streptavidin antibody

Alternative names for Streptavidin antibody

StAv

SwissProt ID

P22629 (Strav)

Available reactivities

All Species, Bac (Bacteria), Hu (Human), S. avidinii (Staphylococcus avidinii)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of Streptavidin antibody

Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Like its namesake avidin, streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature (Kd~10e-15). Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes (Chaiet and Wolf, 1964).
In ELISA-based diagnostic systems, antibodies directed against a particular antigen may be covalently attached to reporter enzymes. Antigens are then quantitated by enzymatic assay after binding to these conjugated molecules. Unfortunately, the precise conditions for accomplishing such covalent attachments must be determined individually for each antibody/reporter combination, and often result in significant loss of either the enzymatic activity of the reporter enzyme or the binding functions of the antibodies. Streptavidin finds utility in these systems because antibody molecules are easily modified by the covalent attachment of derivatives of biotin with little or no loss in the ability of the antibody molecules to bind their antigens. These biotinylated antibodies may be detected by their interaction with conjugates of streptavidin and the reporter enzymes (Guesdon et al, 1979). The same preparation of conjugated streptavidin-reporter enzyme may be used with any number of different biotinylated antibodies making this system a highly flexible one.
The reporter molecule may be bound to streptavidin covalently, or biotinylated and attached to streptavidin via the streptavidin-biotin interaction. Since streptavidin is multivalent (binding 4 molecules of biotin per tetrameric protein molecule) it may be used in combination with biotinylated antibody and biotinylated reporter enzymes to obtain amplified signals. Such amplification in ELISA's is otherwise difficult to obtain and requires the introduction of additional antibody components. ELISA systems employing streptavidin can readily detect sub-nanogram amounts of antigens.

General readings

General / background references:Titus JA et al. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies. J Immunol Methods 50:193-204 (1982). PubMed PMID: 6806389

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Streptavidin antibody

Western blot analysis: Recombinant Streptavidin protein (17kDa) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Streptavidin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 1C2 Purified E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Streptavidin antibody

Western blot analysis: Recombinant Streptavidin protein (17kDa) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Streptavidin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 1C2 Purified E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Streptavidin antibody

Antibody Sensitivity: Streptavidin (10ng/lane) was separated by non-reducing SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20223PU-N Streptavidin antibody (Clone 36G3) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: 1 µg/ml
Lane 2: 0.5 µg/ml
Lane 3: 0.25µg/ml
Lane 4: 0.1 µg/ml
Lane 5: 50 ng/ml
Lane 6: 10 ng/ml
Lane 7: 5 ng/ml Mouse IgG1 36G3 Purified E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Streptavidin antibody

Streptavidin Mouse IgG1 Strep-10 Ig Fraction C, E, P
1 ml / €420.00
  Acris Antibodies GmbH

Streptavidin antibody

Streptavidin Rabbit IgG Serum Dot, E, IP, WB
2 ml / €440.00
  Acris Antibodies GmbH

Streptavidin antibody

Streptavidin Rabbit IgG Texas Red S. avidinii Dot, E, F
20 mg / €3,300.00
  Acris Antibodies GmbH

Streptavidin antibody

Recombinant Streptavidin protein ( 17 kDa ) was resolved by SDS-PAGE and probed with STAV monoclonal antibody, clone 1C2 ( Cat # MAB2046 ) ( 1:2000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 1C2 E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Streptavidin antibody

Streptavidin Mouse IgG1 S3E11 Purified E
1 mg / €320.00
  HyTest Ltd.

Streptavidin antibody

Streptavidin Mouse IgG1 S8C12 Purified E
1 mg / €320.00
  HyTest Ltd.

Streptavidin antibody

Streptavidin Mouse IgG1 S8E4 Purified E
1 mg / €320.00
  HyTest Ltd.

Streptavidin antibody

Streptavidin Mouse IgG1 S10D4 Purified E
1 mg / €320.00
  HyTest Ltd.

Streptavidin antibody

Streptavidin Rabbit IgG FITC All Species E, ICC/IF
4 mg / €930.00
  Novus Biologicals Inc.

Streptavidin antibody

Western Blot: Streptavidin Antibody [Texas Red] [NB120-7240] Biotin conjugated Rabbit anti-trypsin inhibitor antibody and Rabbit anti streptavidin were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit and imaged on the Doc System Rabbit IgG Texas Red Bac E, ICC/IF, WB
4 mg / €930.00
  Novus Biologicals Inc.

Streptavidin antibody

Western Blot: Streptavidin Antibody [Biotin] [NB120-7241] - Streptavidin Biotin Conjugated Antigen: Lane 1 - Biotin conjugated Streptavidin Antibody  Lane 2 - Streptavidin Antibody Load: 3-fold serial dilution starting at 200 ng Secondary antibody: HRP Streptavidin at 1:40,000 for 60 min at Rabbit IgG Biotin All Species E, WB
2 mg / €620.00
  Novus Biologicals Inc.

Streptavidin antibody

Streptavidin Mouse IgG1 S8C12 Purified E, F, P, WB
0.5 mg / €340.00
  Novus Biologicals Inc.

Streptavidin antibody

Streptavidin Mouse IgG1 S8E4 Purified All Species C, E, F, IP, P, WB
0.5 mg / €340.00
  Novus Biologicals Inc.

Streptavidin antibody

Streptavidin Mouse IgG1 S10D4 Purified All Species C, P, WB
0.5 mg / €340.00
  Novus Biologicals Inc.

Streptavidin antibody

Streptavidin Mouse S3E11 Purified Hu C, P, WB
0.5 mg / €340.00
  Novus Biologicals Inc.

Streptavidin antibody

Streptavidin Mouse IgG2b 1C2 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Streptavidin (25-183, His-tag)

Streptavidin Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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