STUB1 / CHIP antibody

Principal name

STUB1 / CHIP antibody

Alternative names for STUB1 / CHIP antibody

NY-CO-7, STIP1 homology and U box-containing protein 1, Carboxy terminus of Hsp70-interacting protein, E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8

SwissProt ID

Q5ZHY5 (Chick), Q9UNE7 (Human), Q9WUD1 (Mouse)

Gene ID

10273 (STUB1), 4710273 (IPR002582)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Dros (Drosophila)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Dot blot (Dot), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of STUB1 / CHIP antibody

The carboxy terminus of Hsc70-interacting protein (CHIP, STUB1) is a co-chaperone protein and functiol E3 ubiquitin ligase that links the polypeptide binding activity of Hsp70 to the ubiquitin proteasome system. Cytoplasmic CHIP protein contains three 34-amino acid TPR (tetratricopeptide repeat) domains at its amino terminus and a carboxy-termil U-box domain. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, while E3 ubiquitin ligase activity is confined to the U-box domain. The binding of CHIP to Hsp70 can stall the folding of Hsp70 client proteins and concomitantly facilitate the U-box dependent ubiquitition of Hsp70-bound substrates. CHIP appears to play a central role in cell stress protection and is responsible for the degradation of disease-related proteins that include cystic fibrosis transmembrane conductance regulator, p53, huntingtin and Ataxin-3, Tau protein, and a-synuclein.

General readings

Jiang J, Ballinger CA, Wu Y, Dai Q, Cyr DM, Hohfeld J, Patterson C.
CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation.
J Biol Chem. 2001 Nov 16;276(46):42938-44.
PMID: 11557750

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

STUB1 / CHIP antibody

CHIP Antibody (C-term) (Cat.# TA324315) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHIP antibody detected the CHIP protein (arrow). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

STUB1 / CHIP antibody

Immunohistochemistry-Paraffin: STUB1 Antibody [NB100-77316] - Mouse teratoma  Affinity_x000d_
purified rabbit anti-STUB1/CHIP used at a dilution of 1:100. Rabbit IgG Aff - Purified Hu ICC/IF, IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

STUB1 / CHIP (C-term) antibody

TA302767 staining (0.3µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

STUB1 / CHIP (C-term) antibody

TA302767 staining (0.3µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

STUB1 / CHIP antibody

Predicted band size: 35 kDa . Positive control: HeLa, PC12, COS1, COS7 and OVCAR3 cell, muscle and liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HeLa cell lysate Lane2: PC12 cell lysate Lane3: COS1 cell lysate Lane4: COS7 cell lysate Lane5: OVCAR3 cell lysate Lane6: Muscle tissue lysate Lane7: Liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

STUB1 / CHIP (C-term) antibody

STUB1 Antibody (C-term) (Cat.# TA324478) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the STUB1 antibody detected the STUB1 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

STUB1 / CHIP antibody

STUB1 / CHIP Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

STUB1 / CHIP antibody

STUB1 / CHIP Mouse Purified Hu, Ms, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

STUB1 / CHIP antibody

STUB1 / CHIP Mouse IgG2a 2E12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

STUB1 / CHIP antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with STUB1 polyclonal antibody ( Cat # PAB1693 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

STUB1 / CHIP antibody

STUB1 polyclonal antibody ( Cat # PAB6119 ) staining ( 0.3 µg/ml ) of human muscle lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

STUB1 / CHIP antibody

recombinant protein; . Lanes: 1:1ug insoluble STUB1 protein, 2:1ug soluble STUB1 protein, 3:1ug EPM2A protein, 4:1ug insoluble PPP1R3C protein, 5:1ug soluble PPP1R3C protein. Primary Antibody Dilution: 1:2500. Secondary Antibody: Anti-rabbit-AP. Secondary Antibody Dilution: 1:20,000. Gene Name: STUB1. Submitted by: Pedro Castanheira, Biocant.; STUB1 antibody - N-terminal region (ARP43058_P050) in recombinant protein cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

STUB1 / CHIP antibody

canine retina; . Lanes: Lane 1: 40ug canine retina lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: STUB1. Submitted by: Dr. Stefania Marsili and Dr. William Beltran, University of Pennsylvania.; STUB1 antibody - C-terminal region (ARP43059_P050) in canine retina cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

STUB1 / CHIP antibody

Western Blot and Immunoprecipitation: STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316. Rabbit IgG Aff - Purified Hu, Ms IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

STUB1 / CHIP antibody

NB300-982 staining (0.3ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

STUB1 / CHIP antibody

Western Blot: STUB1 Antibody [NBP1-30936] - Western blot analysis of STUB1 antibody on 293T (lane A) and Hep G2 (lane B), diluted at 1:1000 with 10% SDS PAGE gel. Rabbit IgG Hu Dot, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

STUB1 / CHIP

STUB1 / CHIP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

STUB1 / CHIP

STUB1 / CHIP Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

STUB1 / CHIP (1-303)

STUB1 / CHIP Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

STUB1 / CHIP (1-303)

STUB1 / CHIP Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

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