SUB1 / PC4 antibody

Principal name

SUB1 / PC4 antibody

Alternative names for SUB1 / PC4 antibody

SUB1 homolog, Positive cofactor 4, RPO2TC1, Activated RNA polymerase II transcriptional coactivator p15

SwissProt ID

P11031 (Mouse), P53999 (Human), P87294 (Schpo), Q5ZK63 (Chick), Q63396 (Rat), Q94045 (Caeel), Q9VLR5 (Drome)

Gene ID

10923 (SUB1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Fi (Fish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SUB1 / PC4 antibody

SUB1, also known as PC4, is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP)-associated factors (TAFs) to directly bind to double stranded DNA. This protein induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Recombinant human SUB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Tavenet A., et al. (2009) Proc Natl Acad Sci U S A. 106(34):14265-70.
Scott KL., et al. (2009) Nature. 459(7250):1085-90.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUB1 / PC4 antibody

Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775] -FFPE section of human prostate carcinoma.   Affinity purified rabbit anti-PC4 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SUB1 / PC4 antibody

WB Suggested Anti-PC4 Antibody Titration: 2.5ug/ml; Positive Control: Raji cell lysateThere is BioGPS gene expression data showing that SUB1 is expressed in Raji Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUB1 / PC4 antibody

WB Suggested Anti-PC4 Antibody Titration: 0.5-1.0ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that SUB1 is expressed in HepG2 Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUB1 / PC4 antibody

SUB1 / PC4 Mouse Hu ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SUB1 / PC4 antibody

SUB1 / PC4 Mouse IgG2a 3A1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUB1 / PC4 antibody

SUB1 / PC4 Mouse IgG2a 2G3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUB1 / PC4 antibody

SUB1 / PC4 Mouse IgG2a 2D6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUB1 / PC4 antibody

Human Lung; PC4 antibody - N-terminal region (P100959_T100) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SUB1 / PC4 antibody

Human Liver; PC4 antibody - middle region (P100960_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SUB1 / PC4 antibody

SUB1 / PC4 Rabbit IgG Aff - Purified Hu WB
50 µg / €680.00
  Funakoshi Co., Ltd.

SUB1 / PC4 antibody

Western Blot and Immunoprecipitation: PC4 Antibody [NB100-59774] - Detection of Human PC4 on HeLa whole cell lysate using NB100-59774. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SUB1 / PC4 antibody

Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SUB1 / PC4

SUB1 / PC4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SUB1 / PC4 (1-127, His-tag)

SUB1 / PC4 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

SUB1 / PC4 (1-127, His-tag)

SUB1 / PC4 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

SUB1 / PC4

SUB1 / PC4 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

SUB1 / PC4

SUB1 / PC4 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

SUB1 / PC4

SUB1 / PC4 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

SUB1 / PC4

SUB1 / PC4 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

SUB1 / PC4

SUB1 / PC4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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