SULF2 antibody

Principal name

SULF2 antibody

Alternative names for SULF2 antibody

KIAA1247, Extracellular sulfatase Sulf-2

SwissProt ID

Q32KJ3, Q8CFG0 (Mouse), Q8IWU5 (Human)

Gene ID

55959 (SULF2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), African clawed frog, Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SULF2 antibody

Sulf-2 exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Heparan sulfate proteoglycans act as co-receptors for numerous heparin-binding growth factors and cytokines and are key regulators of cell signaling. Growth factor binding to heparan sulfate results in mitogenic activity only when specific structural features are present within the heparan sulfate chains. These features include sulfation at specific positions within a disaccharide (N, 2-O, 3-O, 6-O) by the enzymes that regulate heparan sulfate synthesis inside the Golgi. Following synthesis and expression, heparan sulfate can be structurally and functionally modified within the extracellular compartment. Enzymes known to have these effects are heparanase, which cleaves heparan sulfate chains into smaller biologically active fragments, and the heparan sulfate 6-O-endosulfatases (Sulfs). The Sulfs represent a new and novel family of enzymes that are secreted via the Golgi and subsequently localized to the cell surface or may be released into the extracellular matrix. Sulf-1 enzyme is required for Wnt-mediated signaling in developing quail muscle. Sulf1 was shown (in quial) to restore bone morphogenetic protein signaling in cells by releasing the functional inhibitor, Noggin, from cell surfaces. Quail Sulf1 can also inhibit growth factor signaling, the removal of the 6-O-sulfation is required for the formation of the FGF HS FR1c ternary complex blocking FGF2 signaling. Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma. Tumors formed by cells expressing Sulf demonstrate enhanced extracellular matrix deposition. The 6-O-sulfation of heparan sulfate of myeloma tumor cells may be a critical factor in determiningand regulating the in vivo growth of this cancer.

General readings

1. Dai Y, Yang Y, MacLeod V, Yue X, Rapraeger AC, Shriver Z, Venkataraman G, Sasisekharan R, Sanderson RD.
HSulf-1 and HSulf-2 are potent inhibitors of myeloma tumor growth in vivo. J Biol Chem. 2005 Dec 2;280(48):40066-73.
PMID: 16192265

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SULF2 antibody

TA305738 (0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SULF2 antibody

WB Suggested Anti-SULF2 Antibody Titration: 1.25ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SULF2 Control Peptide

SULF2 Purified
50 µg / €210.00
  Acris Antibodies GmbH

SULF2 antibody

Western blot of endogenous Sulf2 in human brain lysate (10 µg/lane) using AP05259PU-N (0.05 µg/ml). Developed with antirabbit HRP (1:5k) and Pierce's Super Signal West Femto. Rabbit IgG Aff - Purified Hu WB
50 µg / €480.00
  Acris Antibodies GmbH

SULF2 antibody

Western blot using antigen immunoaffinity purified anti-human Sulf2 antibody (0.15 µg/ml) on human brain lysate (20 µg/lane) and developed using goat anti rabbit HRP (1:30,000)Pierce's Super Signal West Femto. Rabbit IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SULF2 (C-term) antibody

SULF2 Antibody (C-term) (Cat. #AP54099PU-N) western blot analysis in mouse heart tissue lysates (35µg/lane).This demonstrates the SULF2 antibody detected the SULF2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SULF2 antibody

SULF2 Mouse IgG2a 2H5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SULF2 antibody

Human kidney; SULF2 antibody - C-terminal region (ARP49338_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SULF2 (N-term) antibody

SULF2 Rabbit Purified Rt ICC/IF, WB
25 µg / €630.00
  Funakoshi Co., Ltd.

SULF2 (C-term) antibody

SULF2 Rabbit Purified Rt ICC/IF, WB
25 µg / €630.00
  Funakoshi Co., Ltd.

SULF2 antibody

Western Blot: Sulfatase 2 Antibody (2B4) - Western blot analysis showing Sulfatase 2 expression in MCF7 conditioned media (CM). In CM, the 75 kDa band is dominant (Tang R, Rosen SD.  J Biol Chem. 2009 Aug 7;284(32):21505-14). Image courtesy of Mark Singer at UCSF. Mouse IgG1 2B4 Hu, Ms C, E, IP, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SULF2 antibody

SULF2 Mouse IgG1 2B4 Purified Hu, Ms IP, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

SULF2 antibody

SULF2 Mouse IgG1 2B4 Purified Hu, Ms E, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SULF2 antibody

SULF2 Mouse IgG1 2B4 Purified Hu, Ms E, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SULF2 antibody

SULF2 Mouse IgG1 2B4 Purified Hu, Ms IP, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

SULF2 antibody

SULF2 Mouse IgG1 2B4 Purified Hu, Ms E, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SULF2 antibody

SULF2 Mouse IgG1 2B4 Hu, Ms E, IP, P, WB
25 µl / €200.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SULF2

SULF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SULF2

SULF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SULF2

SULF2 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

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