SUMO1 antibody

Principal name

SUMO1 antibody

Alternative names for SUMO1 antibody

SMT3C, SMT3H3, UBL1, GMP1, SMT3 homolog 3, Sentrin, Small ubiquitin-related modifier 1, GAP-modifying protein 1, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1

SwissProt ID

A7WLH8 (Pig), P63165 (Human), P63166 (Mouse), Q5E9D1 (Bovin), Q5I0H3 (Rat), Q6DEP7 (Xentr)

Gene ID

7341 (SUMO1)

Available reactivities

Ye (Yeast), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Sheep, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection))

Background of SUMO1 antibody

Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein: DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (Yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IκB-α, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12kDa and human SUMO-1 (a 101 amino acid polypeptide) shares 50% sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18% homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with beta-sheets wrapped around an alpha-helix.

General readings

Evdokimov E., et al. (2008), J Cell Sci, 121:4106-13.
Hecker CM., et al. (2006). J Biol Chem. 281(23):16117-27.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUMO1 antibody

SUMO1 Antibody (N-term) (Cat. #TA324522) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SUMO1 antibody detected the SUMO1 protein (arrow). Rabbit IgG Purified Bov, Hu, Ms, Por, Rt ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with SUMO1 Antibody Cat.-No AM50183PU (Clone SPM571). Mouse IgG1 SPM571 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

SUMO1 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with SUMO1 Antibody Cat.-No AM50183PU (Clone SPM571). Mouse IgG1 SPM571 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

SUMO1 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with SUMO1 Antibody Cat.-No AM50183PU (Clone SPM571). Mouse IgG1 SPM571 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

SUMO1 antibody

Western blot analysis of sumo in HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

HeLa cells were arrested in S phase using thymidine, or synchronized in mitosis by a thymidine and nocodazole block followed by release from nocodazole for the indicated time (hr). Cell lysates were spearated by SDS-PAGE and analyzed by immunoblotting with anti-SUMO1 (Image courtesy of Zhang X. D. et al. 2008. Mol Cell 29, 729-741.) Mouse IgG1 Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Western blot analysis of extracts from 293 cells, using Sumo1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

WB of hSUMO fusion protein. Anti-SUMO antibody, generated by immunization with recombinant human SUMO, was tested by WB against a SUMO-GFP fusion protein after cleavage by proteases. Dilution of the antibody between 1:1,000 and 1:5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead). In this blot the antibody was used at a 1:2000 dilution. Rabbit Purified Hu E, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung and ovarian cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human lung and ovarian cancer tissue using SUMO1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Predicted band size: 12 kDa . Positive control: Jukart and A549 cell lysate. Recommended dilution: 1/100-500. (Gel: %SDS-PAGE Lane1: Jukart cell lysate Lane2: A549 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Predicted cell location: Nucleus,Cytoplasm. Positive control: Human ovarian and lung cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SUMO1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Western blot analysis of extracts of HeLa cell line, using SUMO1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Western blot analysis on 293 cell lysate using Sumo1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUMO1 antibody

Figure 1. Western blot using SUMO1 monoclonal antibody (Cat. #AM11043PU) to detect SUMO1 in HL60 cell lysate. Mouse IgG1 66AT1273.94 Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SUMO1 antibody

Immunocytochemical detection of SUMO-1 on 4% PFA fixed HA-tagged SUMO-1 transfected 293T cells with AM26638AF-N. Mouse IgG1 5B12 Azide Free Hu, Ms, Rt ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SUMO1 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with SUMO1 Antibody Cat.-No AM50308PU (Clone SM1/495). Mouse IgG1 SM1/495 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

SUMO1 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with SUMO1 Antibody Cat.-No AM50308PU (Clone SM1/495). Mouse IgG1 SM1/495 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

SUMO1 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with SUMO1 Antibody Cat.-No AM50308PU (Clone SM1/495). Mouse IgG1 SM1/495 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

SUMO1 (N-term) antibody

Immunocytochemical staining of HL-60 cells with Rabbit anti Human SUMO-1 (AP05599PU-N) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

SUMO1 (N-term) antibody

Immunocytochemical staining of HL-60 cells with Rabbit anti Human SUMO-1 (AP05599PU-N) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €280.00
  Acris Antibodies GmbH

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