SUMO3 / SMT3A antibody

Principal name

SUMO3 / SMT3A antibody

Alternative names for SUMO3 / SMT3A antibody

SUMO-3, SMT3 homolog 1, SMT3H1, Small ubiquitin-related modifier 3, Ubiquitin-like protein SMT3A

SwissProt ID

P55854 (Human), Q17QV3 (Bovin), Q5XIF4 (Rat), Q5ZHQ1 (Chick), Q6DI05 (Danre), Q7SZ22 (Xenla), Q9Z172 (Mouse)

Gene ID

6612 (SUMO3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish), Eq (Equine), Prim (Primate), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SUMO3 / SMT3A antibody

Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to human. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c- Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IκB-α, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12kDa and human SUMO-1 (a 101 amino acid polypeptide) shares 50% sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18% homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with β-sheets wrapped around an α-helix.

General readings

1. Muller, S. , Hoege, C. , Pyrowolakis, G. and Jentsch, S. (2001) SUMO, ubiquitin's mysterious cousin. Nat Rev Mol Cell Biol, 2(3): 202-10.
2. Hochstrasser, M. (2001) SP-RING for SUMO: new functions bloom for a ubiquitin-like protein. Cell, 107(1): 5-8.
Kahyo, T.,Nishida, T. and Yasuda, H. (2001) Involvement of PIAS1 in the sumoylation of tumor suppressor p53. Mol Cell, 8(3) 713-8.
3. Takahashi, Y., Kahyo, T., Toh-E, A.,Yasuda, H. and Kikuchi, Y. (2001) Yeast Ull1/Siz1 is a novel SUMO1/Smt3 ligase for septin components and
functions as an adaptor between conjugating enzyme and substrates. J Biol Chem 276(52): 48973-7.
4. Yeh, E.T., Gong, L. and Kamitani, T. (2000) Ubiquitin-like proteins: new wines in new bottles. Gene, 248(1-2):1-14.
5. Keane, M.M., Ettenberg, S.A., Nau, M.M., Banerjee, P., Cuello, M., Penninger, J., and Lipkowitz, S. (1999) cbl-3: a new mammalian cbl family protein.
Oncogene, 18(22):3365-75.
6. Liakopoulos D et al. (1998). A novel protein modification pathway related to the ubiquitin system. EMBO J. 15;17(8):2208-14.
7. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
8. Subramanian,L., Benson,M.D. and Iniguez-Lluhi,J.A. (2003) A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein
alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3. J. Biol. Chem. 278 (11), 9134-9141.
9. Su,H.L. and Li,S.S. (2002) Molecular features of human ubiquitin-like SUMO genes and their encoded proteins. Gene 296 (1-2), 65-73.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUMO2/3 (Center) antibody

(LEFT) Western Blot analysis using anti-SUMO2/3 Pab (Cat.# AP11222PU-N) to detect SUMO2/3 in Saos-2 cell lysate. (RIGHT) Western blot analysis of SUMO3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Center) (Cat.#AP11222PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SUMO3 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SUMO2/3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SUMO3 / SMT3A antibody

WB analysis using Anti-Human SUMO-3 antibody to detect GFP-SUMO fusion proteins (arrowheads). Panel A. Increasing concentrations of human GFP-SUMO-3 with anti-hSUMO-3 antibody at 1:1000. Panel B. Specificity of the antibody was confirmed by SDS-PAGE of various GFP-SUMO constructs followed by transfer onto nitrocellulose. Lanes: 1.Marker, 2. GFP-human SUMO-3, 3. GFP-human SUMO-1, 4. GFP-yeast SUMO, 5. GFP-Arabidopsis thaliana, SUMO-1, 6. GFP- Arabidopsis thaliana SUMO-2, 7. GFP-tomato SUMO. Rabbit Purified Hu E, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

SUMO3 / SMT3A (C-term) antibody

Western blot analysis of SUMO3 in mouse liver tissue lysate with SUMO3 antibody at (A) and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUMO3 / SMT3A antibody

Western blot analysis of extracts of MCF7 cell line, using SUMO3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUMO3 / SMT3A antibody

Western Blot: Sumo 3 Antibody [TA337022] - Analysis of SUMO3 (SMT3) in human testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse testis lysate using SUMO3 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and Pico Rabbit Purified Eq, Hu, Ms, Prim WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUMO3 / SMT3A antibody

SUMO3 / SMT3A Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SUMO3 / SMT3A antibody

WB Suggested Anti-SUMO3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysateSUMO3 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SUMO3 / SMT3A antibody

Figure 1. Western blot using SUMO3 monoclonal antibody (Cat. #AM11045PU) to detect GST-SUMO3 fusion protein (left) and SUMO3 in Jurkat cell lysate (right). Mouse IgG1 76AT630.91.31 Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SUMO3 / SMT3A antibody

SUMO3 / SMT3A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SUMO3 / SMT3A antibody

Immunofluorescence of purified MaxPab antibody to SUMO3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

SUMO3 / SMT3A antibody

Immunofluorescence of purified MaxPab antibody to SUMO3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SUMO3 / SMT3A antibody

SUMO3 / SMT3A Mouse IgG2b 4B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUMO3 / SMT3A antibody

Western Blot analysis of SUMO3 expression in transfected 293T cell line by SUMO3 monoclonal antibody (M08), clone 4G11.

Lane 1: SUMO3 transfected lysate(11.6 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUMO3 / SMT3A antibody

Formalin-fixed and paraffin-embedded human hepatocellular cancer tissue reacted with SUMO3 polyclonal antibody ( Cat # PAB1859 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SUMO3 / SMT3A antibody

Western blot analysis is shown using SUMO3 polyclonal antibody ( Cat # PAB10320 ) to detect GFP-SUMO fusion proteins ( arrowheads ).
Panel A. Increasing concentrations of human GFP-SUMO3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1 : 1000 dilution of anti-SUMO3 antibody in 5% non-fat dry milk in TTBS.
Detection occurred using a 1 : 1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection.
Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 µg of various GFP-SUMO constructs followed by transfer onto nitrocellulose.
Lanes :  1, MW marker. 2, GFP-human SUMO3. 3, GFP-human SUMO1. 4, GFP-yeast SUMO. 5, GFP-Arabidopsis thaliana SUMO1. 6, GFP- Arabidopsis thaliana SUMO2. 7, GFP-tomato SUMO.
After blocking for 1 hour with 5% non-fat dry milk in TTBS,the blot was probed overnight at 4°C with SUMO3 polyclonal antibody diluted and detected as above.
Only the human GFP-SUMO3 band was visualized by chemiluminescence, and no crossreactivity with other SUMO family members was observed. Hu
0.5 mg / €560.00
  Abnova Taiwan Corp.

SUMO3 / SMT3A antibody

Human MCF7; WB Suggested Anti-SUMO3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate. SUMO3 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SUMO3 antibody - middle region (ARP58247_P050) in Human MCF7 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

SUMO3 / SMT3A antibody

Western blot analysis of SUMO3 expression in HeLa (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SUMO3 / SMT3A antibody

Western blot analysis of SUMO3 expression in HeLa (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SUMO3 / SMT3A antibody

Western blot analysis of SUMO3 expression in HeLa (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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