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Superoxide Dismutase 1 / SOD1 antibody

Principal name

Superoxide Dismutase 1 / SOD1 antibody

Alternative names for Superoxide Dismutase 1 / SOD1 antibody

SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1, Superoxide dismutase [Cu-Zn]

SwissProt ID

P00441 (Human), P00442 (Bovin), P07632 (Rat), P08228 (Mouse), P09212 (Rabit), P09670 (Sheep)

Gene ID

6647 (SOD1), 281495, 20655 (Sod1), 24786

Ncbi ID

NP_000445.1, NP_000445, NP_777040, 9606, 10090, 9913, 10116, NP_035564.1, NM_011434, NM_017050

Available reactivities

Eq (Equine), GP (Guinea Pig), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Gt (Goat), Ye (Yeast), Chimp (Chimpanzee), Salmon, Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), Flow Cytometry (F)

Background of Superoxide Dismutase 1 / SOD1 antibody

Cu/Zn-superoxide dismutases (Cu/ Zn-SOD, SOD1) are metalloen zymes involved in the mechanisms of cellular defense against oxidative damage. They have been found in the cytoplasm of all the eukaryotic cells and in the periplasm of several bacterial species. Human SOD1 (approx. MW=19 kDa) is homodimers that contain one atom of zinc and one atom of copper per subunit and catalyze the dismutation of the superoxide anion (O 2 - •) quite efficiently at a rate close to the diffusion limit, over the entire pH range from 5 to 10.:
2O 2 - •+2H + → O 2 +H 2 O 2
(SOD1)
SOD1 possess a very compact and stable structure that is highly resistant to urea, SDS and proteolytic enzymes. SOD1 homodimer consist of two pi eces of eight-stranded β -barrel structure monomer close pack aged on each hydrophobic interface stabilized by intras ubunit disulfid e bond. SOD1 catalyzes the disproportion of superoxide anions via the alternate reduction and reoxidation of the copper ion at the active site of the enzyme. Recent studies in yeast have identified a protein termed the copper chaperone for superoxide dismutase (CCS) as the factor responsible for copper incorporation into SOD1 through direct protein-protein interaction. SO D1's endogenous role remains poorly understood. Mice lacking this enzyme were apparently healthy and displayed no increased sensitivity to hyperoxia. However, they exhibited a pronounced susceptibility to paraquat which induce acute oxidative stress in mice. As a relation to diseas e, elevated level of SOD1 is reported in Down syndrome patients as a result of increase of genedosage (trisomy). Moreover, linkage studies have revealed that mutations in SOD1 are responsible for 10-15% of case of the fetal motor neuron disease familial amyotrophic lateral sclerosis (FALS). Evidence from transgenic mice expressing FALS associated SOD1 mutations, as well as mice homozygous for a deletion of the SOD1 gene, indicates that this neuronal degeneration arises from a gain-of-function associated with the SOD1 mutations rather than loss-of-function. In addition to the legitimate dismutation reaction, SOD1 is able to catalyze surrogate reactions such as the production of •OH using anionic scavengers and H 2 O 2 and nitration of the tyrosine residues of proteins by peroxynitrate. These reactions might be the causes of cytotoxicity.

General readings

Hays,A.P., J. Neurol. Sci. 242 (1-2), 67-69 (2006)

AP00192PU-N - Superoxide Dismutase 1 / SOD1

Western blot analysis of SOD expression with Jurkat cell lysates (Lane 1, 2).

AP00192PU-N

AM01230PU-N - Superoxide Dismutase 1 / SOD1

Immunohistochemistry: AM01230PU-N SOD1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Liver at 15 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AM01230PU-N

Immunohistochemistry: AM01230PU-N SOD1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney at 15 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AM01230PU-N

AP03021PU-S - Superoxide Dismutase 1 / SOD1

Western blot analysis of Cu/Zn SOD, rat, in a human cell line mix using a 1:1000 dilution of the antibody

AP03022PU-S - Superoxide Dismutase 1 / SOD1

Western blot analysis of Cu/Zn SOD in a human cell line mix, using a 1:1000 dilution of the antibody

ICC localization of human Cu/Zn SOD in the syncytiotrophoblast of human placenta. Courtesy of Joan Telfer, University of Glasgow, UK.

AR03033PU-N - Superoxide Dismutase 1 / SOD1

Western blot detection of human SOD1 using rabbit SOD1 polyclonal antibody ( Cat # PAB0724 ) at a dilution of 1 in 500. This antibody detects a band of 16 KDa in human Jurkat T cell extracts ( lane 2 ) . Its specificity for human SOD1 is shown by the lack of reactivity with mouse brain tissue extracts ( lane 1 ) .

PAB0724

Western blot detection of mouse Sod1 in mouse brain tissue extracts ( lane 1 ) when using rabbit Sod1 polyclonal antibody ( Cat # PAB0725 ) at a dilution of 1 : 500 . The immunoreactivity of this antibody is completely abolished when it is pre - incubated with the immunising Sod1 peptide ( lane 2 ) .

PAB0725

Immunoblot analysis of cell and tissue lysates. Lane 1 : Mouse brain. Lane 2 : Mouse liver. Lane 3 : HeLa cell lysate. Lane 4 : 293T cell lysate. Lane 5 : Jurkat cell lysate.

PAB1193

SOD1 polyclonal antibody ( Cat # PAB7007 ) ( 0.02 µg/ml ) staining of mouse brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7007

Immunoprecipitation analysis of Hela cell lysates. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with SOD1 monoclonal antibody, clone 72B1 ( Cat # MAB0684 ).

MAB0684

Immunoprecipitation analysis of Hela cell lysates. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with SOD1 monoclonal antibody, clone 8A1 ( Cat # MAB0688 ).

MAB0688

H00006647-B01P - Superoxide Dismutase 1 / SOD1

SOD1 MaxPab polyclonal antibody. Western Blot analysis of SOD1 expression in human liver.

H00006647-B01P

Western Blot analysis of SOD1 expression in transfected 293T cell line (H00006647-T02) by SOD1 MaxPab polyclonal antibody.Lane 1: SOD1 transfected lysate(16.94 KDa).Lane 2: Non-transfected lysate.

H00006647-B01P

H00006647-D01P - Superoxide Dismutase 1 / SOD1

Western Blot analysis of SOD1 expression in transfected 293T cell line (H00006647-T02) by SOD1 MaxPab polyclonal antibody.Lane 1: SOD1 transfected lysate(15.90 KDa).Lane 2: Non-transfected lysate.

H00006647-D01P

ARP45752_T100 - Superoxide Dismutase 1 / SOD1

Human kidney; SOD1 antibody - N-terminal region (ARP45752_T100) in Human kidney cells using Immunohistochemistry

ARP45752_T100

Human; . Lanes: Lane 1: 50ug HeLa lysate. Lane 2: 50ug 293T lysate. Lane 3: 50ug K562 lysate. Lane 4: 50ug MDA-MB-231 lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:1000. Gene Name: SOD1. Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences .; SOD1 antibody - N-terminal region (ARP45752_T100) in Human cells using Western Blot

ARP45752_T100

Human Jurkat; WB Suggested Antibody Titration: 2.5 ug/ml. Positive Control: Jurkat. SOD1 is supported by BioGPS gene expression data to be expressed in Jurkat.; SOD1 antibody - N-terminal region (ARP45752_T100) in Human Jurkat cells using Western Blot

ARP45752_T100

Western blot analysis of SOD1 expression in Jurkat cell lysates ( Lane 1 and lane 2 ).

PAB9030

AP18205PU-N - Superoxide Dismutase 1 / SOD1

Western blot analysis of SOD1 Antibody (Center) (AP18205PU-N) in Jurkat cell line lysates (35ug/lane). SOD1 (arrow) was detected using the purified Pab.

AP18205PU-N

AP22845PU-N - Superoxide Dismutase 1 / SOD1

Human Liver (formalin-fixed, paraffin-embedded) stained with SOD1 antibody AP22845PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22845PU-N

AM06340SU-N - Superoxide Dismutase 1 / SOD1

Confocal immunofluorescence analysis of 3T3-L1 cells using SOD1 antibody Cat.-No AM06340SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

AM06340SU-N

Confocal Immunofluorescence analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 antibody Cat.-No AM06340SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

AM06340SU-N

Western blot analysis using SOD1 antibody Cat.-No AM06340SU-N against Hela (LAne 1), NIH/3T3 (LAne 2), A549 (Lane 3) and A431 (Lane 4) cell lysate.

AM06340SU-N

AM26611AF-N - Superoxide Dismutase 1 / SOD1

Western blot analysis of SOD1 expression in Jurkat (1) Raji (2), HeLa (3), WR19L (4) and PC12 (5) using AM26611AF-N

AM26611AF-N

R1111HRPS - Superoxide Dismutase 1 / SOD1

Western blot of HRP conjugated Superoxide Dismutase antibody Cat.-No. R1111HRP). Lane 1: Superoxide Dismutase load: 50 ng per lane. Primary antibody: HRP conjugated Superoxide Dismutase antibody at 1/1,000 overnight at 4°C. Secondary antibody: none; HRP substrate. Predicted/observed size: 15.6 kDa, 15.6 kDa.

R1111HRPS

CPA2092-100ul - Superoxide Dismutase 1 / SOD1

Western blot analysis of SOD1 expression in HeLa (A); MCF7 (B); Jurkat (C); HepG2 (D); DU145 (E) whole cell lysates.

TA500495 - Superoxide Dismutase 1 / SOD1

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500495)

TA500495

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500495)

TA500495

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500495)

TA500495

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOD1 (RC200725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD1.

TA500495

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SOD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

TA500495

HEK293T cells transfected with either RC200725 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SOD1 antibody(TA500495), and then analyzed by flow cytometry.

TA500495

TP300725 - Superoxide Dismutase 1 / SOD1

TP300725

TP720521 - Superoxide Dismutase 1 / SOD1

TP720521

TA302692 - Superoxide Dismutase 1 / SOD1

TA302692 (0.02µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA302692

TA302692 (0.01µg/ml) staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA302692

TA321133 - Superoxide Dismutase 1 / SOD1

Predicted band size: 16 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1160 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 45 seconds)

TA321133

TA326813 - Superoxide Dismutase 1 / SOD1

Western blot analysis of extracts of Hela and MCF7 cells, using SOD1 antibody.

TA326813

Immunohistochemistry of paraffin-embedded rat brain using SOD1 antibody at dilution of 1:200 (400x lens).

TA326813

Immunohistochemistry of paraffin-embedded human stomach cancer using SOD1 antibody at dilution of 1:200 (400x lens).

TA326813

TA338376 - Superoxide Dismutase 1 / SOD1

Lanes: Lane 1: 50ug HeLa lysate; Lane 2: 50ug 293T lysate; Lane 3: 50ug K562 lysate; Lane 4: 50ug MDA-MB-231 lysate; Primary Antibody Dilution: 1: 500; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 1000; Gene Name: SOD1; Submitted b

TA338376

WB Suggested Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat.SOD1 is supported by BioGPS gene expression data to be expressed in Jurkat

TA338376

Human kidney

TA338376

TA343111 - Superoxide Dismutase 1 / SOD1

Host: Rabbit; Target Name: SOD1; Sample Tissue: Placenta lysates; Antibody Dilution: 1.0 ug/ml

TA343111

TA347910 - Superoxide Dismutase 1 / SOD1

Western blot analysis of SOD1 expression in Jurkat cells

TA347910

CPA9446-100ul - Superoxide Dismutase 1 / SOD1

Western blot analysis of SOD1 expression in Hela (A); MCF7 (B); mouse brain (C); rat brain (D) whole cell lysates.

Immunohistochemical analysis of SOD1 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA326595	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit Polyclonal Anti-SOD (Cu/Zn) Antibody
	Rabbit	IgG		Aff - Purified	Bov, Hu, Ms, Rt	C, E, IP, WB	0.1 mg / €426.00
							OriGene Technologies, Inc.
	Published recently!
TA500495	Superoxide Dismutase 1 / SOD1 antibody
	SOD1 (Superoxide Dismutase 1) mouse monoclonal antibody, clone OTI8B10 (formerly 8B10)
	Mouse	IgG2b	OTI8B10	Purified	Hu	F, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+5 additional images	Highly validated antibody!
TA302692	Superoxide Dismutase 1 / SOD1 antibody
	Goat Polyclonal Antibody against SOD1
	Goat			Aff - Purified	Can, Hu, Ms, Rt	E, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
+1 additional image
TA321133	Superoxide Dismutase 1 / SOD1 antibody
	Anti-SOD1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA326384	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit polyclonal SOD (Cu/Zn) Antibody
	Rabbit	IgG		Aff - Purified	Bov, Can, Coral, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen	C, E, ICC/IF, IP, WB	0.1 mg / €426.00
							OriGene Technologies, Inc.
+1 additional image
TA326813	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit anti-SOD1 Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA338376	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit Polyclonal Anti-SOD1 Antibody
	Rabbit	IgG		Purified	Hu, Ye	P, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
+2 additional images
TA343111	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit Polyclonal Anti-SOD1 Antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze	WB	50 µg / €360.00
							OriGene Technologies, Inc.
TA347910	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit Polyclonal Anti-SOD1 Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 mg / €335.00
							OriGene Technologies, Inc.
AM01230PU-N	Superoxide Dismutase 1 / SOD1 antibody
	Mouse	IgG1	2F5	Purified	Bov, Hu, Ms	C, E, P	0.1 mg / €420.00
							Acris Antibodies GmbH
+1 additional image
AM06340SU-N	Superoxide Dismutase 1 / SOD1 antibody
	Mouse	IgG1	6F5	Ascites	Hu, Ms	E, ICC/IF, WB	0.1 ml / €400.00
							Acris Antibodies GmbH
+2 additional images
AM26611AF-N	Superoxide Dismutase 1 / SOD1 antibody
	Mouse	IgG1	1G2	Azide Free	Hu	WB	0.1 mg / €390.00
							Acris Antibodies GmbH
AP00192PU-N	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit			Aff - Purified	Eq, GP, Hu, Ms, Rt	C, IP, P, WB	0.1 mg / €340.00
							Acris Antibodies GmbH
AP18205PU-N	Superoxide Dismutase 1 / SOD1 (Center) antibody
	Rabbit				Hu	WB	0.4 ml / €370.00
							Acris Antibodies GmbH
AP22845PU-N	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit			Purified	Eq, GP, Hu, Ms, Rt	C, IP, P, WB	50 µg / €380.00
							Acris Antibodies GmbH
BP825	Superoxide Dismutase 1 / SOD1 antibody
	Sheep	IgG		Purified	Bov, Hu	C, E	1 ml / €400.00
							Acris Antibodies GmbH
R1111	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit	IgG		Serum	Bov	E, WB	2 ml / €1,180.00
							Acris Antibodies GmbH
R1111A	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit	IgG		Azide Free	Bov	Dot, E, ICC/IF, ID, IP, R, WB	10 mg / €240.00
							Acris Antibodies GmbH
R1111B	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit	IgG		Biotin	Bov	Dot, E, ICC/IF, ID, IP, R, WB	1 ml / €360.00
							Acris Antibodies GmbH
R1111HRPS	Superoxide Dismutase 1 / SOD1 antibody
	Rabbit	IgG		HRP	Bov	E, WB	0.1 ml / €460.00
							Acris Antibodies GmbH

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