Superoxide Dismutase 1 / SOD1 antibody

Principal name

Superoxide Dismutase 1 / SOD1 antibody

Alternative names for Superoxide Dismutase 1 / SOD1 antibody

SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1, Superoxide dismutase [Cu-Zn]

SwissProt ID

P00441 (Human), P00442 (Bovin), P07632 (Rat), P08228 (Mouse), P09212 (Rabit), P09670 (Sheep)

Gene ID

6647 (SOD1), 281495, 20655 (Sod1), 24786

Available reactivities

Eq (Equine), GP (Guinea Pig), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Hst (Hamster), Gt (Goat), Ye (Yeast), Chimp (Chimpanzee), Salmon, Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R)

Background of Superoxide Dismutase 1 / SOD1 antibody

Cu/Zn-superoxide dismutases (Cu/ Zn-SOD, SOD1) are metalloen zymes involved in the mechanisms of cellular defense against oxidative damage. They have been found in the cytoplasm of all the eukaryotic cells and in the periplasm of several bacterial species. Human SOD1 (approx. MW=19 kDa) is homodimers that contain one atom of zinc and one atom of copper per subunit and catalyze the dismutation of the superoxide anion (O 2 - •) quite efficiently at a rate close to the diffusion limit, over the entire pH range from 5 to 10.:
2O 2 - •+2H + → O 2 +H 2 O 2
(SOD1)
SOD1 possess a very compact and stable structure that is highly resistant to urea, SDS and proteolytic enzymes. SOD1 homodimer consist of two pi eces of eight-stranded β -barrel structure monomer close pack aged on each hydrophobic interface stabilized by intras ubunit disulfid e bond. SOD1 catalyzes the disproportion of superoxide anions via the alternate reduction and reoxidation of the copper ion at the active site of the enzyme. Recent studies in yeast have identified a protein termed the copper chaperone for superoxide dismutase (CCS) as the factor responsible for copper incorporation into SOD1 through direct protein-protein interaction. SO D1's endogenous role remains poorly understood. Mice lacking this enzyme were apparently healthy and displayed no increased sensitivity to hyperoxia. However, they exhibited a pronounced susceptibility to paraquat which induce acute oxidative stress in mice. As a relation to diseas e, elevated level of SOD1 is reported in Down syndrome patients as a result of increase of genedosage (trisomy). Moreover, linkage studies have revealed that mutations in SOD1 are responsible for 10-15% of case of the fetal motor neuron disease familial amyotrophic lateral sclerosis (FALS). Evidence from transgenic mice expressing FALS associated SOD1 mutations, as well as mice homozygous for a deletion of the SOD1 gene, indicates that this neuronal degeneration arises from a gain-of-function associated with the SOD1 mutations rather than loss-of-function. In addition to the legitimate dismutation reaction, SOD1 is able to catalyze surrogate reactions such as the production of •OH using anionic scavengers and H 2 O 2 and nitration of the tyrosine residues of proteins by peroxynitrate. These reactions might be the causes of cytotoxicity.

General readings

Kurobe N et al. Sensitive enzyme immunoassay for human Cu/Zn superoxide dismutase. Clin Chim Acta 187:11-20 (1990). PubMed PMID: 2138526
Kurobe N et al. Sensitive enzyme immunoassay for human Mn superoxide dismutase. Clin Chim Acta 192:171-9 (1990). PubMed PMID: 2286014
Wong GH et al. Manganous superoxide dismutase is essential for cellular resistance to cytotoxicity of tumor necrosis factor. Cell 58:923-31 (1989). PubMed PMID: 2476237

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SOD1 Antibody Set

SOD1 Antibody Set Rabbit Serum Hu WB
1 kit / €2,410.00
  AgriSera AB

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of Cu/Zn SOD, rat, in a human cell line mix using a 1:1000 dilution of the antibody Rabbit IgG Aff - Purified Bov, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of Cu/Zn SOD, rat, in a human cell line mix using a 1:1000 dilution of the antibody Rabbit IgG Aff - Purified Bov, Hu, Ms, Rt C, E, IP, WB
25 µg / €240.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500495) Mouse IgG2b OTI8B10 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Superoxide Dismutase 1 / SOD1 antibody

Immunofluorescence: Superoxide Dismutase 1 Antibody (6F5) [NBP1-47443] - Confocal immunofluorescence analysis of 3T3-L1 cells using anti-SOD1 mAb (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 6F5 Purified Hu, Ms E, F, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Superoxide Dismutase 1 / SOD1 antibody

Immunohistochemistry-Paraffin: Superoxide Dismutase 1 Antibody (8B10) [NBP1-47993] - Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SOD1mouse monoclonal antibody. (Dilution 1:50) Mouse 8B10 Aff - Purified Hu F, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

Superoxide Dismutase 1 / SOD1 antibody

TA302692 (0.02µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

TA302692 (0.02µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Predicted band size: 16 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1160 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 45 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of Cu/Zn SOD in a human cell line mix, using a 1:1000 dilution of the antibody Rabbit IgG Aff - Purified Bov, Can, Coral, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen C, E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of extracts of Hela and MCF7 cells, using SOD1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Lanes: Lane 1: 50ug HeLa lysate; Lane 2: 50ug 293T lysate; Lane 3: 50ug K562 lysate; Lane 4: 50ug MDA-MB-231 lysate; Primary Antibody Dilution: 1: 500; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 1000; Gene Name: SOD1; Submitted b Rabbit IgG Purified Hu, Ye P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Host: Rabbit; Target Name: SOD1; Sample Tissue: Placenta lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of SOD1 expression in Jurkat cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Superoxide Dismutase 1 / SOD1 antibody

Immunohistochemistry: AM01230PU-N SOD1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Liver at 15 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 2F5 Purified Bov, Hu, Ms C, E, P
0.1 mg / €410.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Confocal immunofluorescence analysis of 3T3-L1 cells using SOD1 antibody Cat.-No AM06340SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 6F5 Ascites Hu, Ms E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of SOD1 expression in Jurkat (1) Raji (2), HeLa (3), WR19L (4) and PC12 (5) using AM26611AF-N Mouse IgG1 1G2 Azide Free Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of SOD expression with Jurkat cell lysates (Lane 1, 2). Rabbit Aff - Purified Eq, GP, Hu, Ms, Rt C, IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 antibody

Western blot analysis of Cu/Zn SOD in a human cell line mix, using a 1:1000 dilution of the antibody Rabbit IgG Aff - Purified Bov, Can, Coral, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen C, E, ICC/IF, IP, WB
25 µg / €240.00
  Acris Antibodies GmbH

Superoxide Dismutase 1 / SOD1 (Center) antibody

Western blot analysis of SOD1 Antibody (Center) (AP18205PU-N) in Jurkat cell line lysates (35ug/lane). SOD1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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