SUPT16H / FACT140 antibody

Principal name

SUPT16H / FACT140 antibody

Alternative names for SUPT16H / FACT140 antibody

FACT complex subunit SPT16, FACTp140, Facilitates chromatin transcription complex subunit SPT16, FACT 140 kDa subunit, Chromatin-specific transcription elongation factor 140 kDa subunit

SwissProt ID

Q920B9 (Mouse), Q9W603 (Xenla), Q9Y5B9 (Human)

Gene ID

11198 (SUPT16H)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Bov (Bovine), Rt (Rat), Ze (Zebrafish), African clawed frog, Por (Porcine), Eq (Equine), Gt (Goat), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of SUPT16H / FACT140 antibody

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H. Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene. Sequence Note: The sequence AF152961.1 is a chimeric mRNA clone. Only the SUPT16H region was propagated into this RefSeq record. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Belotserkovskaya R, Oh S, Bondarenko VA, Orphanides G, Studitsky VM, Reinberg D. FACT facilitates transcription-dependent nucleosome alteration. Science. 2003 Aug 22;301(5636):1090-3. PMID: 12934006

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUPT16H / FACT140 antibody

Formalin-Fixed Paraffin-Embedded Human Liver stained with SUPT16H Antibody Cat.-No AM08331PU (Clone 8D2) at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 8D2 Purified Hu ICC/IF, IP, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

SUPT16H / FACT140 antibody

Formalin-Fixed Paraffin-Embedded Human Liver stained with SUPT16H Antibody Cat.-No AM08331PU (Clone 8D2) at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 8D2 Purified Hu ICC/IF, IP, WB
25 µg / €210.00
  Acris Antibodies GmbH

SUPT16H / FACT140 antibody

TA303070 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

WB Suggested Anti-SUPT16H Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate; SUPT16H is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

WB using the antibody against SPT16 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right; the molecular weight marker is shown on the left. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

WB using the antibody against SPT16 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker is shown on the left. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

Western blot analysis on HepG2 cell lysate using SUPT16H Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Rabbit IgG Purified Hu, Ms WB
0.1 mg / €360.00
  Acris Antibodies GmbH

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Rabbit IgG Purified Hu, Ms IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

SUPT16H / FACT140 (321-640) antibody

Figure 1. Staining SUPT16H in Breast by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu, Ms IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

SUPT16H monoclonal antibody (M01), clone 1D12 Western Blot analysis of SUPT16H expression in Hela NE ( Cat # L013V3 ). Mouse IgG2a 1D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Mouse IgG2a 2D1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Mouse IgG2b 1F1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Mouse IgG2b 3G9 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Mouse IgG2a 4D6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

SUPT16H polyclonal antibody ( Cat # PAB6901 ) ( 0.1 µg/ml ) staining of HeLa cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Hu, Ms
0.1 mg / €400.00
  Abnova Taiwan Corp.

SUPT16H / FACT140 antibody

Western blot analysis of extracts from HepG2/Jurkat cells, using SUPT16H Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

SUPT16H / FACT140 antibody

Western blot analysis of extracts from HepG2/Jurkat cells, using SUPT16H Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

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