SUPT16H / FACT140 antibody

Principal name

SUPT16H / FACT140 antibody

Alternative names for SUPT16H / FACT140 antibody

FACT complex subunit SPT16, FACTp140, Facilitates chromatin transcription complex subunit SPT16, FACT 140 kDa subunit, Chromatin-specific transcription elongation factor 140 kDa subunit

SwissProt ID

Q920B9 (Mouse), Q9W603 (Xenla), Q9Y5B9 (Human)

Gene ID

11198 (SUPT16H), 114741 (Supt16h)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Gt (Goat), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SUPT16H / FACT140 antibody

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H. Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene. Sequence Note: The sequence AF152961.1 is a chimeric mRNA clone. Only the SUPT16H region was propagated into this RefSeq record. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Heo,K., (2008) Mol. Cell 30 (1), 86-97

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUPT16H / FACT140 antibody

  Goat Polyclonal Antibody against SUPT16H  
TA303070 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

  Rabbit Polyclonal Anti-SUPT16H Antibody  
WB Suggested Anti-SUPT16H Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate; SUPT16H is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

  Rabbit Polyclonal Spt16 Antibody  
WB using the antibody against SPT16 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right; the molecular weight marker is shown on the left. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

  Rabbit Polyclonal Spt16 Antibody  
WB using the antibody against SPT16 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker is shown on the left. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

  Rabbit Polyclonal Anti-SUPT16H Antibody  
Western blot analysis on HepG2 cell lysate using SUPT16H Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

  Mouse Monoclonal anti-Spt16 (FACT140 complex) Antibody  
Western blot analysis of Jurkat (lane 1), Hela (lane 2), HEK293 (lane 3) and NIH3T3 (lane 4) cells using anti-Spt16 (FACT140) antibody (8D2). GAPDH antibody (poly6314) was used as loading control. Mouse 8D2 Purified Hu ICC/IF, IP, WB
25 µg / €248.00
  OriGene Technologies, Inc.

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Rabbit IgG Purified Hu, Ms WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SUPT16H / FACT140 antibody

SUPT16H / FACT140 Rabbit IgG Purified Hu, Ms IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SUPT16H / FACT140 (321-640) antibody

Figure 1. Staining SUPT16H in Breast by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu, Ms IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SUPT16H / FACT140 antibody

Western blot analysis of extracts from HepG2/Jurkat cells, using SUPT16H Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €230.00
  Assay Biotech

SUPT16H / FACT140 antibody

Western blot analysis of extracts from HepG2/Jurkat cells, using SUPT16H Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

SUPT16H - human gene knockout kit via CRISPR

SUPT16H - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SUPT16H - human gene knockout kit via CRISPR

SUPT16H - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SUPT16H - human gene knockout kit via CRISPR

SUPT16H - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SUPT16H - human gene knockout kit via CRISPR

SUPT16H - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Supt16 - mouse gene knockout kit via CRISPR

Supt16 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Supt16 - mouse gene knockout kit via CRISPR

Supt16 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Supt16 - mouse gene knockout kit via CRISPR

Supt16 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Supt16 - mouse gene knockout kit via CRISPR

Supt16 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SUPT16H (mGFP-tagged)-Human suppressor of Ty 16 homolog (S. cerevisiae) (SUPT16H), 200 uL, >10^7 TU/mL

Lenti ORF particles, SUPT16H (mGFP-tagged)-Human suppressor of Ty 16 homolog (S. cerevisiae) (SUPT16H), 200 uL, >10^7 TU/mL  
200 µl / €2,120.00
  OriGene Technologies, Inc.

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