SUPT3H / SPT3 antibody

Principal name

SUPT3H / SPT3 antibody

Alternative names for SUPT3H / SPT3 antibody

SPT3-like protein, SPT3L, Transcription initiation protein SPT3 homolog

SwissProt ID

O75486 (Human)

Gene ID

8464 (SUPT3H)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SUPT3H / SPT3 antibody

The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from δ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.

General readings

Yu,J., et al., (1998) Genomics 53 (1), 90-96

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUPT3H / SPT3 antibody

  SUPT3H mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SUPT3H mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808395)(1:150) Mouse IgG1 OTI1A5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SUPT3H / SPT3 antibody

  Rabbit Polyclonal anti-SUPT3H antibody  
WB Suggested Anti-SUPT3H Antibody Titration: 0.4ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysateSUPT3H is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUPT3H / SPT3 antibody

  Rabbit Polyclonal Anti-SUPT3H Antibody  
Western blot analysis on HuvEc cell lysate using SUPT3H Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUPT3H / SPT3 antibody

  SUPT3H mouse monoclonal antibody, clone OTI3D1 (formerly 3D1)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SUPT3H mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808117)(1:150) Mouse IgG1 OTI3D1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SUPT3H / SPT3 antibody

  SUPT3H mouse monoclonal antibody, clone OTI4A6 (formerly 4A6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT3H (RC208269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT3H.(1:2000) Mouse IgG1 OTI4A6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SUPT3H / SPT3 antibody

Western blot (WB) analysis of SPT3 antibody (Cat.-No.: AP06645PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SUPT3H / SPT3 antibody

Western blot analysis of extracts from HuvEc/HepG2 cells, using SUPT3H Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

SUPT3H / SPT3 antibody

Western blot analysis of extracts from HuvEc/HepG2 cells, using SUPT3H Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

SUPT3H - human gene knockout kit via CRISPR

SUPT3H - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SUPT3H - human gene knockout kit via CRISPR

SUPT3H - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SUPT3H - human gene knockout kit via CRISPR

SUPT3H - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SUPT3H - human gene knockout kit via CRISPR

SUPT3H - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SUPT3H / SPT3 (full length, N-term HIS tag, transcript variant 2)

  Recombinant protein of human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
SUPT3H / SPT3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SUPT3H overexpression lysate

SUPT3H overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SUPT3H (mGFP-tagged) - Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, SUPT3H (mGFP-tagged) - Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, SUPT3H (Myc-DDK tagged) - Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, SUPT3H (Myc-DDK tagged) - Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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