SUPT3H / SPT3 antibody
Principal name
SUPT3H / SPT3 antibody
Alternative names for SUPT3H / SPT3 antibody
SPT3-like protein, SPT3L, Transcription initiation protein SPT3 homolog
SwissProt ID
O75486 (Human)
Gene ID
8464 (SUPT3H)
Ncbi ID
9606, NP_003590, NP_003590.1, ABZ92456.1, NM_181356, NP_852001, NM_003599
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SUPT3H / SPT3 antibody
The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from δ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.
General readings
Yu,J., et al., (1998) Genomics 53 (1), 90-96
Human Liver; Rabbit Anti-SUPT3H Antibody. Catalog Number: ARP30038. Paraffin Embedded Tissue: Human Liver. Cellular Data: Hepatocytes. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SUPT3H antibody - N-terminal region (ARP30038_T100) in Human Liver cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-SUPT3H Antibody Titration: 0.4ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate. SUPT3H is supported by BioGPS gene expression data to be expressed in HepG2.; SUPT3H antibody - N-terminal region (ARP30038_T100) in Human HepG2 cells using Western Blot
Western blot (WB) analysis of SPT3 antibody (Cat.-No.: AP06645PU-N) in extracts from HUVEC cells.
SUPT3H monoclonal antibody (M01A), clone 1A6 Western Blot analysis of SUPT3H expression in HeLa ( Cat # L013V1 ).
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SUPT3H mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808117)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SUPT3H mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808117)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT3H (RC208269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT3H.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT3H (RC208269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT3H.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SUPT3H mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808395)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SUPT3H mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808395)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SUPT3H mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808395)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SUPT3H mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808395)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SUPT3H mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808395)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SUPT3H mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808395)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT3H (RC208269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT3H.(1:2000)
Immunohistochemical analysis of SUPT3H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SUPT3H staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of SUPT3H expression in A431 (A); K562 (B); HL60 (C) whole cell lysates.
Western blot analysis on HuvEc cell lysate using SUPT3H Antibody
WB Suggested Anti-SUPT3H Antibody Titration: 0.4ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysateSUPT3H is supported by BioGPS gene expression data to be expressed in HepG2
Rabbit Anti-SUPT3H Antibody; Paraffin Embedded Tissue: Human Liver; Cellular Data: Hepatocytes; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
