SUPT3H / SPT3 antibody
Principal name
SUPT3H / SPT3 antibody
Alternative names for SUPT3H / SPT3 antibody
SPT3-like protein, SPT3L, Transcription initiation protein SPT3 homolog
SwissProt ID
O75486 (Human)
Gene ID
8464 (SUPT3H)
Ncbi ID
9606, NP_003590, NP_003590.1, ABZ92456.1, NM_181356, NP_852001, NM_003599
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SUPT3H / SPT3 antibody
The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from δ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.
General readings
Yu,J., et al., (1998) Genomics 53 (1), 90-96
















