SUPT4H1 antibody

Principal name

SUPT4H1 antibody

Alternative names for SUPT4H1 antibody

SPT4, SPT4H, SUPT4H, DSIFp14, Transcription elongation factor SPT4, DRB sensitivity-inducing factor small subunit

SwissProt ID

P63272 (Human), Q3SYX6 (Bovin), Q5HZ97 (Xenla), Q6DGQ0 (Danre), Q9TVQ5 (Drome), Q9TZ93 (Caeel)

Gene ID

6827 (SUPT4H1)

Available reactivities

Hu (Human), Bov (Bovine), Mky (Monkey), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of SUPT4H1 antibody

SUPT4H1 is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mR processing and transcription elongation by R polymerase II. DSIF positively regulates mR capping by stimulating the mR guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptiol pausing at sites proximal to the promoter. Transcriptiol pausing may facilitate the assembly of an elongation competent R polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to R polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by R polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through tural pause sites. DSIF can also positively regulate transcriptiol elongation and is required for the efficient activation of transcriptiol elongation by the HIV-1 nuclear transcriptiol activator, Tat. DSIF acts to suppress transcriptiol pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at termitor sequences.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUPT4H1 antibody

Host: Rabbit; Target Name: Supt4h1; Sample Tissue: Rat Pancreas lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SUPT4H1 antibody

SUPT4H1 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SUPT4H1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT4H1 (RC201211, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT4H1. Mouse IgG1 OTI6B10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SUPT4H1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT4H1 (RC201211, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT4H1. Mouse IgG1 OTI5F12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SUPT4H1 antibody

SUPT4H1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SUPT4H1 antibody

SUPT4H1 Mouse IgG2a 3G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SUPT4H1 (Center) antibody

Human 293T; WB Suggested Anti-SUPT4H1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; SUPT4H1 antibody - middle region (ARP38258_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

SUPT4H1 antibody

Western blot analysis of SUPT4H expression in HeLa (A); NS-1 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SUPT4H1 antibody

Western blot analysis of SUPT4H expression in HeLa (A); NS-1 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SUPT4H1 antibody

Western blot analysis of SUPT4H expression in HeLa (A); NS-1 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SUPT4H1

SUPT4H1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SUPT4H1

SUPT4H1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SUPT4H1

SUPT4H1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SUPT4H1

SUPT4H1 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

SUPT4H1

SUPT4H1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SUPT4H1

SUPT4H1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SUPT4H1

SUPT4H1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SUPT4H1

SUPT4H1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SUPT4H1 overexpression lysate

SUPT4H1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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