SUV39H1 / KMT1A antibody

Principal name

SUV39H1 / KMT1A antibody

Alternative names for SUV39H1 / KMT1A antibody

SUV39H, Lysine N-methyltransferase 1A, Histone-lysine N-methyltransferase SUV39H1, Histone H3-K9 methyltransferase 1, Position-effect variegation 3-9 homolog, Suppressor of variegation 3-9 homolog 1

SwissProt ID

O43463 (Human), O54864 (Mouse), Q2NL30 (Bovin), Q6NRE8 (Xenla)

Gene ID

6839 (SUV39H1), 20937 (Suv39h1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Rb (Rabbit), Eq (Equine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SUV39H1 / KMT1A antibody

In eukaryotic cells the histone methylase KMT1A / SUV39H1 and the methyl-lys binding protein HP1 interact to repress transcription at heterochromatic sites. Lys 9 of histone H3 is methylated by KMT1A, creating a binding site for the chromo domain of HP1. The Lys methylase activity of KMT1A resides in the SET domain. KMT1A and HP1 are both involved in the repressive functions of the retinoblastoma (Rb) protein. KMT1A cooperates with Rb to repress the cyclin E promoter, and in fibrobroblasts that are disrupted for KMT1A, the activity of the cyclin E and cyclin A2 genes are specifcally elevated. Chromatin IPs (ChIPs) show that Rb is necessary to direct methylation of histone H3, and is necessary for binding of HP1 to the cyclin E promoter. The KMT1A-HP1 complex is thus not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other co-repressor proteins. Histone H3 lysine 9 can be mono, di or tri methylated. Different HMTases have recently been found responsible for each modification state. G9a is thought to be responsible for all mono and di methylation of H3 K9 in slient regions of heterochromatin. In contrast, KMT1A and H2 are thought to be responsible fro the tri methylation of H3K9 found in pericentric heterochromatin.

General readings

General / background referencesPeters AH et al. Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability. Cell 107323-37 (2001). PubMed PMID: 11701123
Rea S et al. Regulation of chromatin structure by site-specific histone H3 methyltransferases. Nature 406593-9 (2000). PubMed PMID: 10949293
Firestein R et al. Set domain-dependent regulation of transcriptional silencing and growth control by SUV39H1, a mammalian ortholog of Drosophila Su(var)3-9. Mol Cell Biol 204900-9 (2000). PubMed PMID: 10848615
Aagaard L et al. Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31. EMBO J 181923-38 (1999). PubMed PMID: 10202156

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SUV39H1 / KMT1A antibody

WB Suggested Anti-SUV39H1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateSUV39H1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SUV39H1 / KMT1A antibody

WB Suggested Anti-SUV39H1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateSUV39H1 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SUV39H1 / KMT1A (N-term) antibody

Figure 1. Western analysis of extracts from 293 cells using SUV39H1 antibody. Mouse IgG1 42AT239.96.72 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SUV39H1 / KMT1A (N-term) antibody

Western blot analysis of anti-SUV39H1 Pab (Cat. #AP11169PU-N) in SAOS-2 cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2). SUV39H1 (arrow) is detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SUV39H1 / KMT1A antibody

Western analysis of extracts from 293 cells using SUV39H1 monoclonal antibody, clone 42AT239.96.72 ( Cat # MAB1156 ) . Mouse IgG1 42AT239.96.72 Purified Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SUV39H1 / KMT1A antibody

Western blot analysis of SUV39H1 polyclonal antibody ( Cat # PAB2362 ) in SAOS-2 cell lysate ( Lane 1 ) and NIH/3T3 cell lysate ( Lane 2 ) . SUV39H1 ( arrow ) is detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Purified Hu, Ms C, E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SUV39H1 / KMT1A antibody

Human Lung; . Rabbit Anti-SUV39H1 Antibody. . Catalog Number:. ARP32470 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SUV39H1 / KMT1A antibody

Human 721_B; WB Suggested Anti-SUV39H1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. SUV39H1 is supported by BioGPS gene expression data to be expressed in 721_B.; SUV39H1 antibody - N-terminal region (ARP32471_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SUV39H1 / KMT1A antibody

SUV39H1 / KMT1A Mouse IgG1 44,1 Hu, Ms E, ICC/IF, IP, WB
50 µl / €340.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SUV39H1 / KMT1A

SUV39H1 / KMT1A Human
2 µg / €300.00
  Abnova Taiwan Corp.

SUV39H1 / KMT1A

SUV39H1 / KMT1A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SUV39H1 / KMT1A

SUV39H1 / KMT1A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SUV39H1 overexpression lysate

SUV39H1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

13 Item(s)

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