swi6 antibody

Principal name

swi6 antibody

Alternative names for swi6 antibody

Chromatin-associated protein swi6

SwissProt ID

P40381 (Schpo)

Gene ID

2541633 (swi6)

Available reactivities

Ye (Yeast), Hu (Human), Ms (Mouse)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP)

Background of swi6 antibody

The chromatin structure of Fission yeast centromeres is regulated epigenetically. In the central core domain, the histone H3 variant Cnp1(CENP-A) plays a key role. In the flanking heterochromatin domain, histones are kept underacetylated by the histone deacetylases (HDACs) Clr3, Clr6 and Sir2, and methylated by Clr4 methyltransferase (HMTase) to create a specific binding site for the Swi6 protein. Swi6 then directly mediates cohesin binding to the centromeric heterochromatin. Recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Involved in the repression of the silent mating-type loci MAT2 and MAT3. May compact MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes. Swi6 interacts with histone H3 methylated at Lys-9 and with the cohesin subunit psc3. Swi6 associates with Swi4 to activate HO and many other late G1-specific transcripts in budding yeast. In contrast, histone H3 methylated at Lys9 and its interacting protein Swi6/HP1, which define heterochromatin, coated extended domains associated with a variety of repeat elements and small islands corresponding to meiotic genes. Contains two chromo domains.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

swi6 (314-328) antibody

Western blot analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S.pombe Swi6 protein. ~35µg of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 µm nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified Ye E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

swi6 antibody

Western blot analysis is shown using swi6 polyclonal antibody ( Cat # PAB11378 ) to detect endogenous protein present in S.pombe lysate ( arrowhead ).
Comparison to a molecular weight marker ( not shown ) indicatesa band of ~43 kDa corresponding to S.pombe Swi6 protein.
Approx. ~35?g of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 µm nitrocellulose.
The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h. Rabbit Hu, Ms E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

swi6 antibody

Western Blot: Swi6 Antibody [NB600-932] - Western blot analysis is shown using  Affinity Purified anti-Swi6 antibody  to detect endogenous protein present in  S.pombe lysate (arrowhead). Comparison to a  molecular weight marker (not shown) indicates  a band of ~43 kDa corresponding to S.pombe  Swi6 protein. ~35μg of lysate was loaded per  lane onto a 4-20% gradient gel for SDS-PAGE  followed by transfer to 0.45 μm nitrocellulose.  The blot was incubated with a 1:1,700 dilution  of the antibody at room temperature for 2 h  followed by detection using IRDye?800  labeled Goat-a-Rabbit IgG [H&L] (611-132-  122) diluted 1:5,000 for 45 min. Rabbit IgG Aff - Purified Ye E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

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