Symplekin / SYMPK antibody

Principal name

Symplekin / SYMPK antibody

Alternative names for Symplekin / SYMPK antibody

SPK

SwissProt ID

Q80X82 (Mouse), Q92797 (Human)

Gene ID

8189 (SYMPK)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of Symplekin / SYMPK antibody

SYMPK is a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3\'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3\'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Cao,Y., (2007) Histopathology 51 (4), 528-538

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  2. Highly Validated (2)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Symplekin / SYMPK antibody

Immunoperoxidase of monoclonal antibody to SYMPK on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml] Mouse IgG1 4C2 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Symplekin / SYMPK antibody

Immunohistochemistry-Paraffin: Symplekin Antibody [NB100-74592] - FFPE section of human pancreatic islet cell tumor.  Affinity purified rabbit anti-Symplekin used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu C, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Symplekin / SYMPK antibody

Symplekin / SYMPK Mouse IgG1 Sym-TJ-E150 Supernatant Hu, Rt C, WB
5 ml / €290.00
  Acris Antibodies GmbH

Symplekin / SYMPK antibody

Symplekin / SYMPK Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Symplekin / SYMPK antibody

Immunofluorescence of purified MaxPab antibody to SYMPK on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Symplekin / SYMPK antibody

Western Blot analysis of SYMPK expression in transfected 293T cell line (H00008189-T02) by SYMPK MaxPab polyclonal antibody.

Lane 1: SYMPK transfected lysate(58.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Symplekin / SYMPK antibody

Human HT29; . Sample Type :. Human HT29 cells. Primary Antibody Dilution :. 1:50. Secondary Antibody :. Donkey anti Rabbit-Alexa Fluor 488. Secondary Antibody Dilution :. 1:500. Color/Signal Descriptions :. Green: Symplekin Blue: DAPI. Gene Name :. SYMPK. Submitted by :. Louise Lagerqvist (PhD), Oncology Department, Institute of Functional Genomics, Montpellier, France.; SYMPK antibody - N-terminal region (ARP42900_P050) in Human HT29 cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

Symplekin / SYMPK antibody

Human HepG2; WB Suggested Anti-SYMPK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. SYMPK is supported by BioGPS gene expression data to be expressed in HepG2.; SYMPK antibody - middle region (ARP42901_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Symplekin / SYMPK antibody

Western Blot: Symplekin Antibody [NB100-74590] - Detection of Human Symplekin by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-Symplekin antibody NB100-74590 used for IP at 3  mcg/mg lysate. Symplekin was also immunoprecipitated by  rabbit anti-Symplekin antibodies NB100-74591 and NB100-74592,  which recognize downstream epitopes.   Detection: Chemiluminescence with  an exposure time of 1 second. Rabbit IgG Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Symplekin / SYMPK antibody

WB and IP: Symplekin Antibody [NB100-74591] - Detection of Human Symplekin on HeLa whole cell lysate using  A301-464A. Symplekin was also IPed by rabbit anti-Symplekin antibodies NB100-74590 and NB100-74592 which recognize other epitopes. Rabbit IgG Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Symplekin / SYMPK antibody

Immunohistochemistry-Paraffin: Symplekin Antibody [NBP1-92464] - Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Symplekin / SYMPK

Symplekin / SYMPK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Symplekin / SYMPK

Symplekin / SYMPK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Symplekin / SYMPK

Symplekin / SYMPK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SYMPK 293T Cell Transient Overexpression Lysate(Denatured)

SYMPK 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

15 Item(s)

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