Bov (Bovine), GP (Guinea Pig), Hu (Human), Ms (Mouse), Rt (Rat), Ge (Gerbil)
Mouse, Rabbit, Guinea Pig
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Synaptopodin / SYNPO antibody
The spine apparatus (SA) is a specialized form of endoplasmic reticulum (ER) that is found in a subpopulation of dendritic spines in central neurons. The SA consists of a series of stacked discs that are thought to be connected to each other and to the dendritic system of ER-tubules. The actin binding protein synaptopodin (which has originally been described in podocytes of the kidney) is an essential component of the SA. Mice that lack the gene for synaptopodin do not form a spine apparatus. The SA is believed to play a critical role in learning and memory. In summary, an important function of the spine apparatus is the regulation of plasticity at individual synapses, a process known as metaplasticity. The International Radiation Hybrid Mapping Consortium mapped the SYNPO gene to chromosome 5.
Staining for Synaptopodin on formalin fixed paraffin embedded kidney tissue sections using Acris Cat.-No BM5086P anti-SYNPO monoclonal antibody following antigen retrieval. Indirect immunoperoxidase staining. DAB substrate, hematoxylene counterstain.
Western blotting for Synaptopodin on Human kidney lysate using Acris Cat.-No BM5086P anti-SYNPO monoclonal antibody. The antibody reacts with a band of approximate size 44kDa that is thought to result from the breakdown of the full length protein.
> 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.